A5031681387- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Genetics and Neurodevelopmental Disorders
- RNA Research and Splicing
- Cancer-related gene regulation
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Substance Abuse Treatment and Outcomes
- Local Government Finance and Decentralization
- Animal Genetics and Reproduction
- Media Influence and Health
- International Development and Aid
- Sexuality, Behavior, and Technology
- Chromatin Remodeling and Cancer
- Quality and Safety in Healthcare
- Bullying, Victimization, and Aggression
- Heat shock proteins research
- Counseling Practices and Supervision
- Genomic variations and chromosomal abnormalities
- Genetics, Aging, and Longevity in Model Organisms
- Spaceflight effects on biology
University of Michigan
2017-2025
Mass General Brigham
2023-2024
Michigan Medicine
2019-2020
ID Genomics (United States)
2019
Northwestern University
2013
Rice Institute
2013
Madison County Health Department
2009
Foreign and Commonwealth Office
1971-1973
Government of the United Kingdom
1973
Genome-wide association studies have revealed that 88% of disease-associated single-nucleotide polymorphisms (SNPs) reside in noncoding regions. However, SNPs remain understudied, partly because they are challenging to prioritize for experimental validation. To address this deficiency, we developed the SNP effect matrix pipeline (SEMpl).SEMpl estimates transcription factor-binding affinity by observing differences chromatin immunoprecipitation followed deep sequencing signal intensity within...
Abstract XX female and XY male therian mammals equalize X-linked gene expression through the mitotically-stable transcriptional inactivation of one two X chromosomes in somatic cells. Here, we describe an essential function homolog ancestral X-Y pair, Kdm5c - Kdm5d , Xist lncRNA, which is required for stable X-inactivation. Ablation females results a significant reduction RNA expression. encodes demethylase that enhances by converting histone H3K4me2/3 modifications into H3K4me1. Ectopic...
Abstract Histone H3 lysine 4 methylation (H3K4me) is extensively regulated by numerous writer and eraser enzymes in mammals. Nine H3K4me are associated with neurodevelopmental disorders to date, indicating their important roles the brain. However, interplay among during brain development remains largely unknown. Here, we show functional interactions of a writer-eraser duo, KMT2A KDM5C , which responsible for Wiedemann-Steiner Syndrome (WDSTS), mental retardation X-linked syndromic...
Transcriptional enhancers enable exquisite spatiotemporal control of gene expression in metazoans. Enrichment monomethylation histone H3 lysine 4 (H3K4me1) is a major chromatin signature transcriptional enhancers. Lysine (K)-specific demethylase 1A (KDM1A, also known as LSD1), an H3K4me2/me1 demethylase, inactivates stem-cell during the differentiation mouse embryonic stem cells (mESCs). However, its role undifferentiated mESCs remains obscure. Here, we show that KDM1A actively maintains...
When engaging in playful teasing, understanding the norms for acceptable teasing is critical. The present study surveyed beliefs concerning prescriptive and descriptive among 101 male 88 female college students. Participants viewed a number of topics as unacceptable indicated that high percentage common were more closely associated with one sex. Both sexes expected men to experience being less upset by be likely reciprocate teasing.
Abstract Transcriptional enhancers enable exquisite spatiotemporal control of gene expression in metazoans. Enrichment mono-methylation histone H3 lysine 4 (H3K4me1) is a major chromatin signature that distinguishes from promoters. Lysine Specific Demethylase 1 (LSD1, aka KDM1A), an enzyme specific for demethylating H3K4me2/me1, has been shown to “decommission” stem cell during the differentiation mouse embryonic cells (mESC). However, roles LSD1 undifferentiated mESC remain obscure. Here,...
Abstract Histone H3 lysine 4 methylation (H3K4me) is extensively regulated by numerous writer and eraser enzymes in mammals. Nine H3K4me are associated with neurodevelopmental disorders to date, indicating their important roles the brain. However, interplay among during brain development remains largely unknown. Here, we show functional interactions of a writer-eraser duo, KMT2A KDM5C , which responsible for Wiedemann-Steiner Syndrome (WDSTS), mental retardation X-linked syndromic...
How cell-type-specific chromatin landscapes emerge and progress during metazoan ontogenesis remains an important question. Transcription factors are expressed in a manner recruit chromatin-regulatory machinery to specific genomic loci. In contrast, proteins broadly assumed exert the same intrinsic function across cell types. However, human genetics studies have revealed unexpected vulnerability of neurodevelopment factor mutations with unknown mechanisms. Here, we report that 14 regulators...
Abstract LSD1 histone H3K4 demethylase and its binding partner PHF21A, a reader protein for unmethylated H3K4, both undergo neuron-specific microexon splicing. The neuronal weakens demethylation activity can alter the substrate specificity to H3K9 or H4K20. Meanwhile, PHF21A interferes with nucleosome binding. However, temporal expression patterns of splicing isoforms during brain development remain unknown. In this work, we report that isoform precedes human neuron differentiation mouse...
I step away from the sterile angels with their stiff white coats. Their eyes spoke for them, and words they did not say. frighten them. remind them that wear no halo, hold magic wand, silver bullets in pockets, today. across threshold, through door standing ajar. Everything looks different—the chair stands too straight, counter sits high, lamp lies shadow. back out, beyond front porch. There are everywhere, curious eyes, ask but don't want an answer, a gavel depths. Caverns am afraid to walk...
An amendment to this paper has been published and can be accessed via a link at the top of paper.
SUMMARY XY male and XX female mammals equalize X-linked gene expression through the mitotically-stable transcriptional inactivation of an X-chromosome in females. Although most genes are silent on inactive-X, some escape silencing expressed at higher levels females vs. males. Here, we show that escapee Smcx / Kdm5c , encoding a histone H3K4me2/3 demethylase, underlies female-specific induction X-inactivation. Mouse embryonic epiblast cells differentiating stem (ESCs) lacking SMCX reduced...