A5043267985- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Advanced Fluorescence Microscopy Techniques
- Nuclear Structure and Function
- Cell Image Analysis Techniques
- Microtubule and mitosis dynamics
- Chromosomal and Genetic Variations
- Single-cell and spatial transcriptomics
- Reproductive Biology and Fertility
- Advanced Electron Microscopy Techniques and Applications
- RNA and protein synthesis mechanisms
- Cellular Mechanics and Interactions
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
- Genetics, Bioinformatics, and Biomedical Research
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Photosynthetic Processes and Mechanisms
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Cellular transport and secretion
- Image Processing Techniques and Applications
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
European Molecular Biology Laboratory
2016-2025
European Molecular Biology Laboratory
2015-2024
European Bioinformatics Institute
2003-2024
Science for Life Laboratory
2024
European Molecular Biology Organization
2007-2022
Harvard University
2021
Stanford University
2019
Rockefeller University
2018
Spanish National Cancer Research Centre
2015
Universidad de Málaga
2015
The generation of mathematical models biological processes, the simulation these processes under different conditions, and comparison integration multiple data sets are explicit goals systems biology that require knowledge absolute quantity system's components. To date, systematic estimates cellular protein concentrations have been exceptionally scarce. Here, we provide a quantitative description proteome commonly used human cell line in two functional states, interphase mitosis. We show...
The mechanisms of localization and retention membrane proteins in the inner nuclear fate this system during mitosis were studied living cells using protein, lamin B receptor, fused to green fluorescent protein (LBR–GFP). Photobleaching techniques revealed majority LBR–GFP be completely immobilized envelope (NE) interphase cells, suggesting a tight binding heterochromatin and/or lamins. A subpopulation within ER membranes, by contrast, was entirely mobile diffused rapidly freely (D = 0.41 ±...
Quantitative time-lapse imaging data of single cells expressing the transmembrane protein, vesicular stomatitis virus ts045 G protein fused to green fluorescent (VSVG–GFP), were used for kinetic modeling traffic through various compartments secretory pathway. A series first order rate laws was sufficient accurately describe VSVG–GFP transport, and provided compartment residence times constants transport into out Golgi complex delivery plasma membrane. For ER mean constant (i.e., fraction...
Chromosome segregation and cell division are essential, highly ordered processes that depend on numerous protein complexes. Results from recent RNA interference screens indicate the identity composition of these complexes is incompletely understood. Using gene tagging bacterial artificial chromosomes, localization, tandem-affinity purification-mass spectrometry, MitoCheck consortium has analyzed about 100 human complexes, many which had not or only been characterized. This work led to...
Much of life's essential molecular machinery consists large protein assemblies that currently pose challenges for structure determination. A prominent example is the nuclear pore complex (NPC), which organization its individual components remains unknown. By combining stochastic super-resolution microscopy, to directly resolve ringlike NPC, with single particle averaging, use information from thousands pores, we determined average positions fluorescent labels in NPC a precision well below 1...
Condensin is a protein complex associated with mitotic chromosomes that has been implicated in chromosome condensation. In vertebrates, two types of condensin complexes have recently identified, called I and II. Here, we show mammalian cells II associates chromatin prophase, contrast to which cytoplasmic can thus interact only after nuclear envelope breakdown. RNA interference experiments conjunction imaging live fixed revealed required for condensation early whereas appears be dispensable...