A5068359135- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Genomics and Chromatin Dynamics
- Advanced biosensing and bioanalysis techniques
- Nanopore and Nanochannel Transport Studies
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- RNA Research and Splicing
- Fungal and yeast genetics research
- Biosensors and Analytical Detection
- Cancer Genomics and Diagnostics
- RNA Interference and Gene Delivery
- Electrowetting and Microfluidic Technologies
- 3D Printing in Biomedical Research
- Gene Regulatory Network Analysis
- Modular Robots and Swarm Intelligence
- Advanced Fluorescence Microscopy Techniques
- Barrier Structure and Function Studies
- Cellular Mechanics and Interactions
- Cell Image Analysis Techniques
- Chromosomal and Genetic Variations
- Innovative Microfluidic and Catalytic Techniques Innovation
- Angiogenesis and VEGF in Cancer
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
Laboratory for Integrated Micro-Mechatronic Systems
2023-2025
Centre National de la Recherche Scientifique
2015-2025
Laboratoire d'Analyse et d'Architecture des Systèmes
2016-2025
The University of Tokyo
2023-2025
Roche (France)
2010-2024
Université de Toulouse
2011-2023
Tokyo University of Science
2023
University of Lausanne
2017
Université Toulouse III - Paul Sabatier
2008-2017
Roche (Switzerland)
2011-2015
When a constant, homogenous magnetic field is applied to suspension of superparamagnetic particles confined in thin gap perpendicular the field, self-organize into fixed, quasi-regular array columns ([Fig. 1][1]A) ([1][2]). The returns liquid
Chromosome dynamics are recognized to be intimately linked genomic transactions, yet the physical principles governing spatial fluctuations of chromatin still a matter debate. Using high-throughput single-particle tracking, we recorded movements nine fluorescently labeled chromosome loci located on chromosomes III, IV, XII, and XIV Saccharomyces cerevisiae over an extended temporal range spanning more than four orders magnitude (10 −2 –10 3 sec). Spatial appear characterized by anomalous...
Genome dynamics are intimately linked to the regulation of gene expression, most fundamental mechanism in biology, yet we still do not know whether very process transcription drives spatial organization at specific loci. Here, have optimized ANCHOR/ParB DNA-labeling system for real-time imaging a single-copy, estrogen-inducible transgene human cells. Motion an ANCHOR3-tagged DNA locus was recorded same cell before and during appearance nascent MS2-labeled mRNA. We found that initiation by...
Abstract Over the next few years, it is expected that new, energetic, multifunctional materials will be engineered. There a need for new methods to assemble such from manufactured nanopowders. In this article, we demonstrate DNA‐directed assembly procedure produce highly energetic nanocomposites by assembling Al and CuO nanoparticles into micrometer‐sized particles of an Al/CuO nanocomposite, which has exquisite performance in comparison with its physically mixed counterparts. Using 80 nm...
The technique of fluorescence recovery after photobleaching (FRAP) was introduced in the mid-1970s to study diffusion biomolecules living cells. For several years, it used mainly by a small number biophysicists who had developed their own systems. Since mid-1990s, FRAP has gained increasing popularity because conjunction two factors: First, techniques are easily implemented on confocal laser-scanning microscopes (CLSMs), and so become available anyone access such equipment. Second, advent...
Abstract DNA folding and dynamics along with major nuclear functions are determined by chromosome structural properties, which remain, thus far, elusive in vivo. Here, we combine polymer modeling single particle tracking experiments to determine the physico-chemical parameters of chromatin vitro living yeast. We find that motion reconstituted fibers can be recapitulated Rouse model using mechanical nucleosome arrays deduced from simulations. Conversely, report shows some inconsistencies...
We present an experimental study of the microfluidic electrophoresis long DNA in self-assembling matrixes magnetic bead columns. Results are presented for rapid separation lambda-phage, 2lambda-DNA, and bacteriophage T4 DNA, where resolutions greater than 2 between lambda achieved times as short 150 s. The use a computer-piloted flow control system injection results high reproducibility separations. compare experimentally measured mobility dispersion with exactly solvable lattice Monte Carlo...
In response to mechanical cues, endothelial cells elicit highly sensitive cellular pathways that contribute the regulation of physiology and disorders vascular system. However, it remains relatively unexplored how tissues process integrate intraluminal pressure, in turn regulate permeation flow across vessel wall. Leveraging a tissue engineering approach create microvessels (MVs), we measured real-time induced by pressures ranging from 0.1 2.0 kPa. Our findings reveal mechanically stimulated...
In response to mechanical cues, endothelial cells elicit highly sensitive cellular pathways that contribute the regulation of physiology and disorders vascular system. However, it remains...
Chromosomes architecture is viewed as a key component of gene regulation, but principles chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics longest chromosome Saccharomyces cerevisiae (XII). Chromosome XII carries ribosomal DNA (rDNA) that defines nucleolus, major hallmark nuclear organization. We determined intranuclear positions 15 loci distributed every ∼100 kb along chromosome, investigated their motion over...
DNA size separation followed by purification and enrichment constitute essential operations for genetic engineering.
In mammals, silencing of one the two X chromosomes in female cells compensates for different number between sexes. The noncoding Xist RNA initiates chromosome inactivation. spreads from its transcription site over territory and triggers formation a repressive chromatin domain. To understand localization we aimed to develop system investigating living cells. Here report successful visualization transgenically expressed MS2-tagged mouse embryonic stem Imaging during an entire cell cycle shows...
We describe a technology to perform sizing and concentration analysis of double stranded DNA with sensitivity 10 fg/μL in an operating time 20 min. The is operated automatically on commercial capillary electrophoresis instrument using electro-hydrodynamic actuation. It relies new device that achieves online at the junction between two capillaries different diameters, thanks viscoelastic lift forces. Using set ladders range 100-1500 bp, we report accuracy precision better than 3%...
We present a novel Lab-on-Chip technology for 3D particle tracking in living cells based on V-shaped micro-mirrors, which are used to observe fluorescent specimens from multiple vantage points, providing simultaneous stereo-images that can be recombined reconstruction. Our readily with standard fluorescence microscopes, and we apply it study chromatin dynamics using yeast strains one or two GFP-tagged gene loci. Using an Andor EMCCD camera, loci followed inter-frame intervals of up 10 ms...