A5034738381- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- RNA modifications and cancer
- Genetic Associations and Epidemiology
- Bacterial Genetics and Biotechnology
- DNA and Nucleic Acid Chemistry
- DNA Repair Mechanisms
- Cancer therapeutics and mechanisms
- Nuclear Structure and Function
- CRISPR and Genetic Engineering
- Trace Elements in Health
- Protein Tyrosine Phosphatases
- Ovarian cancer diagnosis and treatment
- Photosynthetic Processes and Mechanisms
- Forensic and Genetic Research
- Nutrition, Genetics, and Disease
- Diabetes Treatment and Management
- Cancer-related molecular mechanisms research
- Enterobacteriaceae and Cronobacter Research
- Advanced Electron Microscopy Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Genetic and phenotypic traits in livestock
- Lipoproteins and Cardiovascular Health
Centre National de la Recherche Scientifique
2011-2025
Université de Toulouse
2011-2025
Travaux et Recherches Archéologiques sur les Cultures, les Espaces et les Sociétés
2025
Université Toulouse III - Paul Sabatier
2000-2024
Institut Agro Montpellier
2019
Laboratoire de Biologie Moléculaire des Eucaryotes
2006-2019
Université de Montpellier
2016-2019
Institut de Recherche pour le Développement
2015-2019
Institut Universitaire de Recherche Clinique
2014-2017
Physiologie de la Nutrition et du Comportement Alimentaire
2017
The formation of silenced and condensed heterochromatin foci involves enrichment protein 1 (HP1). HP1 can bridge chromatin segments form liquid droplets, but the biophysical principles underlying compartmentalization in cell nucleus are elusive. Here, we assess mechanistically relevant features pericentric compaction mouse fibroblasts. We find that (1) has only a weak capacity to droplets living cells; (2) size, global accessibility, independent HP1; (3) lack separated pool; (4) toggle...
RNA polymerase I (Pol I) produces large ribosomal RNAs (rRNAs). In this study, we show that the Rpa49 and Rpa34 Pol subunits, which do not have counterparts in II III complexes, are functionally conserved using heterospecific complementation of human Schizosaccharomyces pombe orthologues Saccharomyces cerevisiae. Deletion RPA49 leads to disappearance nucleolar structure, but assembly can be restored by decreasing gene copy number from 190 25. Statistical analysis Miller spreads absence...
Abstract DNA folding and dynamics along with major nuclear functions are determined by chromosome structural properties, which remain, thus far, elusive in vivo. Here, we combine polymer modeling single particle tracking experiments to determine the physico-chemical parameters of chromatin vitro living yeast. We find that motion reconstituted fibers can be recapitulated Rouse model using mechanical nucleosome arrays deduced from simulations. Conversely, report shows some inconsistencies...
Ribosome biogenesis is a major metabolic effort for growing cells. In Saccharomyces cerevisiae, Hmo1, an abundant high-mobility group box protein (HMGB) binds to the coding region of RNA polymerase I transcribed ribosomal RNAs genes and promoters ∼70% genes. this study, we have demonstrated functional conservation eukaryotic HMGB proteins involved in DNA (rDNA) transcription. We shown that when expressed budding yeast, human UBF1 newly identified Sp-Hmo1 (Schizosaccharomyces pombe) localize...
Chromosomes architecture is viewed as a key component of gene regulation, but principles chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics longest chromosome Saccharomyces cerevisiae (XII). Chromosome XII carries ribosomal DNA (rDNA) that defines nucleolus, major hallmark nuclear organization. We determined intranuclear positions 15 loci distributed every ∼100 kb along chromosome, investigated their motion over...
ABSTRACT How spatial organization of the genome depends on nuclear shape is unknown, mostly because accurate size and measurement technically challenging. In large cell populations yeast Saccharomyces cerevisiae, we assessed geometry (size shape) nuclei in three dimensions with a resolution 30 nm. We improved an automated fluorescence localization method by implementing post-acquisition correction spherical microscopic aberration along z-axis, to detect dimensional (3D) positions pore...
The Marine Isotope Stage 3 is a context of considerable climatic instability. Establishing the link between global climate changes and their impact on local ecological contexts prey exploited by human populations challenging. Still, it necessary to understand better conditions where humans lived unravel how they adapted fluctuating environmental conditions. Here, we address this question studying 250 osteodental elements from animals hunted consumed groups at Isturitz, rich well-documented...
The association of RNA polymerase III (Pol III)-transcribed genes with nucleoli seems to be an evolutionarily conserved property the spatial organization eukaryotic genomes. However, recent studies global chromosome architecture in budding yeast have challenged this view. We used live-cell imaging determine intranuclear positions 13 Pol III-transcribed genes. frequency nucleolus and nuclear periphery depends on linear genomic distance from tethering elements-centromeres or telomeres....
Branched chain amino acids (BCAA) are essential elements of the human diet, which display increased plasma levels in obesity and regained particular interest as potential biomarkers for development diabetes. To define determinants insulin resistance (IR) we investigated 73 genes involved BCAA metabolism or regulation by fine-scale haplotype mapping two European populations with metabolic syndrome. French Romanians (n = 465) were genotyped SNPs (Affymetrix) enriched imputation (BEAGLE 4.1) at...
Most transcriptional activity of exponentially growing cells is carried out by the RNA Polymerase I (Pol I), which produces a ribosomal (rRNA) precursor. In budding yeast, Pol multimeric enzyme with 14 subunits. Among them, Rpa49 forms Rpa34 I-specific heterodimer (homologous to PAF53/CAST in human might be responsible for specific functions I. Previous studies provided insight involvement initiation, elongation, docking and releasing Rrn3, an essential transcription factor. Here, we took...
During ribosome biogenesis, the RNA precursor to mature rRNAs undergoes numerous post-transcriptional chemical modifications of bases, including conversions uridines pseudouridines. In archaea and eukaryotes, these are performed by box H/ACA small ribonucleoprotein particles (box RNPs), which contain a guide responsible for selection substrate four proteins, pseudouridine synthase, Cbf5p. So far, no in vitro reconstitution eukaryotic RNPs from purified components has been achieved,...
In DNA site-specific recombination catalysed by tyrosine recombinases, two pairs of strands are sequentially exchanged between separate duplexes and the mechanisms that confer directionality to this theoretically reversible reaction remain unclear. The recombinase TnpI acts at internal resolution site (IRS) transposon Tn4430 resolve intermolecular transposition products. Recombination is IRS core sites (IR1–IR2) regulated adjacent TnpI-binding motifs (DR1 DR2). These dispensable accessory...
Abstract Chromosome mechanical properties determine DNA folding and dynamics, underlie all major nuclear functions. Here we combine modeling real-time motion tracking experiments to infer the physical parameters describing chromatin fibers. In vitro, of nucleosome arrays can be accurately modeled by assuming a Kuhn length 35-55 nm. vivo , amplitude chromosome fluctuations is drastically reduced, depends on transcription. Transcription activation increases dynamics only if it involves gene...
Transcription is a major contributor to genomic instability. The ribosomal RNA (rDNA) gene locus consists of head-to-tail repeat the most actively transcribed genes in genome. polymerase I (RNAPI) responsible for massive rRNA production, and nascent co-transcriptionally assembled with early assembly factors yeast nucleolus. In
Abstract Most transcriptional activity of exponentially growing cells is carried out by the RNA Polymerase I (Pol I), which produces a ribosomal (rRNA) precursor. In budding yeast, Pol multimeric enzyme with 14 subunits. Among them, Rpa49 forms Rpa34 I-specific heterodimer (homologous to PAF53/CAST in human might be responsible for specific functions I. Previous studies provided insight involvement initiation, elongation, docking and releasing Rrn3, an essential transcription factor. Here,...