A5086501436- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Fungal and yeast genetics research
- DNA Repair Mechanisms
- Helicobacter pylori-related gastroenterology studies
- HIV Research and Treatment
- Microscopic Colitis
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Hepatitis Viruses Studies and Epidemiology
- CRISPR and Genetic Engineering
- Forensic and Genetic Research
- Cholesterol and Lipid Metabolism
- Hepatitis B Virus Studies
- Digital and Cyber Forensics
- Animal Virus Infections Studies
- HIV/AIDS drug development and treatment
- Edible Oils Quality and Analysis
- Porphyrin Metabolism and Disorders
- Fullerene Chemistry and Applications
- Histone Deacetylase Inhibitors Research
- Phytochemistry and Bioactivity Studies
- Hepatitis C virus research
- Epigenetics and DNA Methylation
The University of Texas Southwestern Medical Center
2020-2025
Southwestern Medical Center
2022-2025
Council of Scientific and Industrial Research
2013-2023
Centre for Cellular and Molecular Biology
2013-2023
Indian Institute of Technology Delhi
2011-2019
Indian Institute of Science Bangalore
2017
National Institute of Technology Rourkela
2014
Despite extensive investigations into the microbiome and metabolome changes associated with colon polyps colorectal cancer (CRC), profiles of individuals colonic polyposis, including those (Gene-pos) without (Gene-neg) a known genetic driver, remain comparatively unexplored. Using biopsies, polyps, stool from patients Gene-pos adenomatous polyposis (N = 9), Gene-neg 18), serrated syndrome (SPS, N 11), we demonstrated through 16S rRNA sequencing that mucosa-associated microbiota in is...
Chromosomes architecture is viewed as a key component of gene regulation, but principles chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics longest chromosome Saccharomyces cerevisiae (XII). Chromosome XII carries ribosomal DNA (rDNA) that defines nucleolus, major hallmark nuclear organization. We determined intranuclear positions 15 loci distributed every ∼100 kb along chromosome, investigated their motion over...
Membrane-active peptides, components of capsid structural proteins, assist viruses in overcoming the host membrane barrier initial stages infection. Several such peptides have been identified, and their roles fusion or disruption characterized through biophysical studies. In several members Picornaviridae family, role VP4 peptide cellular-membrane penetration is well established. However, there not much information on membrane-penetrating hepatitis A virus (HAV), an unusual member this...
Thymic hypoplasia, hypoparathyroidism, cardiac defects, and/or dysmorphic facial features are frequent congenital malformations resulting from chromosome 22q11.2 deletion syndrome (22q11.2DS; aka DiGeorge syndrome). Thymus hypoplasia results in reduced peripheral T cells, with patients suffering more and severe infections. Embryonic thymuses mouse models of 22q11.2DS (Tbx1neo2/neo2) smaller than controls. Such had a distinct mesenchymal cell subset representation, altered transcriptomes,...
Patients with mutations in various inositol tris-phosphate receptor (ITPR) family members display diverse clinical phenotypes. We report on two unrelated individuals the ITPR3 p.Arg2524Cys variant who have growth retardation, immunodeficiency, partial anhidrosis, and peripheral neuropathy. To delineate mechanisms leading to these phenotypes, a mouse line was developed genocopy human p.Arg2524Cys. Mice heterozygous for homologous Itpr3 p.Arg2523Cys were small, had severe B cell lymphopenia,...
Abstract Viroporins are virally encoded, membrane-active proteins, which enhance viral replication and assist in egress of viruses from host cells. The 2B proteins the picornaviridae family known to have viroporin-like properties play critical roles during virus replication. protein Hepatitis A Virus (2B), an unusual picornavirus, is somewhat dissimilar its analogues several respects. HAV approximately 2.5 times length other does not disrupt calcium homeostasis or glycoprotein trafficking....
The association of RNA polymerase III (Pol III)-transcribed genes with nucleoli seems to be an evolutionarily conserved property the spatial organization eukaryotic genomes. However, recent studies global chromosome architecture in budding yeast have challenged this view. We used live-cell imaging determine intranuclear positions 13 Pol III-transcribed genes. frequency nucleolus and nuclear periphery depends on linear genomic distance from tethering elements-centromeres or telomeres....
HIV-1 latency remains a barrier to functional cure because of the ability virtually silent yet inducible proviruses within reservoir cells transcriptionally reactivate upon cell stimulation. reactivation occurs through sequential action host transcription factors (TFs) during “host phase” and viral TF Tat “viral phase”, which together facilitate positive feedback loop required for exponential transcription, replication, pathogenesis. The these TFs poses challenge precisely delineate...
The influence of the titanium (Ti) interlayer thickness on growth and electron emission characteristics carbon nanotubes (CNTs) deposited silicon (Si) coated with an iron (Fe) catalyst layer was investigated. Ti films 5 nm, 10 15 nm in were beneath Fe layers. Multiwalled (MWCNTs) via microwave plasma enhanced chemical vapor deposition. hinders diffusion into substrate thus helps MWCNTs. In addition, role as a sacrificial catalytic diffusion, surface morphology, microstructure, and, thus,...
Abstract The RNA polymerase (pol) III transcribes mostly short, house-keeping genes, which produce stable, non-coding RNAs. tRNAs highly transcribed by pol in vivo are known replication fork barriers. One of the transcription factors, PAF1C (RNA II associated factor 1 complex) is reported to associate with I and influence their transcription. We found low level occupancy on yeast III-transcribed not correlated nucleosome positions, interacts complex causes loss from genes under stress....
Abstract Control of RNA Polymerase II (pol II) elongation is a critical component gene expression in mammalian cells. The PNUTS-PP1 complex controls rates, slowing pol after polyadenylation sites to promote termination. Kaposi’s sarcoma-associated herpesvirus (KSHV) co-opts express its genes, but little known about regulation elongation. We identified PNUTS as suppressor KSHV reporter genome-wide CRISPR screen. depletion enhances global and overall viral replication. Mechanistically,...
The FACT (FAcilitates Chromatin Transactions) complex influences transcription initiation and enables passage of RNA polymerase (Pol) II through gene body nucleosomes during elongation. In the budding yeast, ∼280 noncoding genes highly transcribed in vivo by Pol III are found nucleosome-free regions bordered positioned nucleosomes. downstream nucleosome dynamics was to regulate via controlling terminator accessibility hence, terminator-dependent recycling. As opposed enrichment at 5′-ends...
Abstract Many regulatory proteins and complexes influence transcription by RNA polymerase (pol) II. In comparison, only a few are known for pol III, which transcribes mostly house-keeping non-coding genes. Yet, III is precisely regulated under various stress conditions like starvation. We used complex components TFIIIC (Tfc6), (Rpc128) TFIIIB (Brf1) as baits to identify potential interactors through mass spectrometry-based proteomics. A large interactome constituting chromatin modifiers,...
Hsp90α is a stress protein that acts as molecular chaperone and known to assist in the maturation, folding stabilization of various cellular proteins ‘client proteins’. However, factors drive interaction between its client are not well understood. In present investigation, we predicted basis different with both wild mutant p53 other proteins. We have presence hydrophobic patches having substantial value hydropathy index minimum percent similarity 40 % necessary condition for be recognized by...