A5045646025- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Cellular Mechanics and Interactions
- RNA and protein synthesis mechanisms
- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Bacterial Genetics and Biotechnology
- Fungal and yeast genetics research
- Hemodynamic Monitoring and Therapy
- Advanced Fluorescence Microscopy Techniques
- Vibrio bacteria research studies
- Cardiomyopathy and Myosin Studies
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- T-cell and B-cell Immunology
- Blood properties and coagulation
- Tissue Engineering and Regenerative Medicine
- DNA Repair Mechanisms
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Inhalation and Respiratory Drug Delivery
- Nuclear Structure and Function
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Elasticity and Material Modeling
- Non-Invasive Vital Sign Monitoring
Université Toulouse III - Paul Sabatier
2013-2023
Centre National de la Recherche Scientifique
2005-2023
Université de Toulouse
2013-2023
Laboratoire de Biologie Moléculaire des Eucaryotes
2008-2021
Laboratoire de Microbiologie et Génétique Moléculaires
2013
Centre National pour la Recherche Scientifique et Technique (CNRST)
2013
Translational Innovation in Medicine and Complexity
2006
Genetic transformation, in which cells internalize exogenous DNA and integrate it into their chromosome, is widespread the bacterial kingdom. It involves a specialized membrane-associated machinery for binding double-stranded (ds) uptake of single-stranded (ss) fragments. In human pathogen Streptococcus pneumoniae, this specifically assembled at competence. The EndA nuclease, constitutively expressed virulence factor, recruited during competence to play key role converting dsDNA ssDNA...
Chromosomes architecture is viewed as a key component of gene regulation, but principles chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics longest chromosome Saccharomyces cerevisiae (XII). Chromosome XII carries ribosomal DNA (rDNA) that defines nucleolus, major hallmark nuclear organization. We determined intranuclear positions 15 loci distributed every ∼100 kb along chromosome, investigated their motion over...
ABSTRACT How spatial organization of the genome depends on nuclear shape is unknown, mostly because accurate size and measurement technically challenging. In large cell populations yeast Saccharomyces cerevisiae, we assessed geometry (size shape) nuclei in three dimensions with a resolution 30 nm. We improved an automated fluorescence localization method by implementing post-acquisition correction spherical microscopic aberration along z-axis, to detect dimensional (3D) positions pore...
Abstract Quantification of cardiomyocyte contraction is usually obtained by measuring globally cell shortening from the displacement extremities. We developed a correlation‐based optical flow method, which correlates whole‐cell temporal pattern with precise quantification intracellular strain wave at sarcomeres level. A two‐dimensional image correlation analysis cardiomyocytes phase‐contrast images was to extract local deformations videomicroscopy time‐lapse sequences. Test images,...
Bacterial genomes typically consist of a single chromosome and, optionally, one or more plasmids. But whole-genome sequencing reveals about ten per-cent them to be multipartite, with additional replicons which by size and indispensability are considered secondary chromosomes. This raises the questions how their replication partition is managed without compromising genome stability such arose. Vibrio cholerae, 1 Mb replicon in addition its 3 chromosome, only species for maintenance...
Inherently dynamic, chromosomes adopt many different conformations in response to DNA metabolism. Models of chromosome organization the yeast nucleus obtained from genome-wide conformation data or biophysical simulations provide important insights into average behavior but fail reveal features dynamic transient events that are only visible a fraction cells at any given moment. We developed method determine relative positions three fluorescently tagged living imaged 3D. Cell type specific...
A precise characterization of cell elastic properties is crucial for understanding the mechanisms by which cells sense mechanical stimuli and how these factors alter cellular functions. Optical magnetic tweezers are micromanipulation techniques widely used quantifying stiffness adherent from their response to an external force applied on a bead partially embedded within cortex. However, relationships between imposed resulting translation or rotation obtained experimental only characterize...
Spatial organisation of chromosomes is a determinant genome stability and required for proper mitotic segregation. However, visualization individual chromatids in living cells quantification their geometry, remains technically challenging. Here, we used live cell imaging to quantitate the three-dimensional conformation yeast Saccharomyces cerevisiae ribosomal DNA (rDNA). rDNA confined within nucleolus composed about 200 copies representing 10% genome. To fluorescently label cells, generated...
The genome is organized in functional compartments and structural domains at the sub-megabase scale. How within these interactions between numerous cis-acting enhancers promoters regulate transcription remains an open question. Here, we determined chromatin folding composition over several hundred kb around estrogen-responsive genes human breast cancer cell lines after hormone stimulation. Modeling of 5C data 1.8 resolution was combined with quantitative 3D analysis multicolor FISH...
REP, diverse palindromic DNA sequences found at high copy number in many bacterial genomes, have been attributed important roles cell physiology but their dissemination mechanisms are poorly understood. They might represent non-autonomous transposable elements mobilizable by TnpAREP, the first prokaryotic domesticated transposase associated with REP. fundamentally different from classical transposases, members of HuH superfamily and closely related to transposases IS200/IS605 family. We...
Abstract The genome is organized in functional compartments and structural domains at the sub-megabase scale. How within these interactions between numerous cis-acting enhancers promoters regulate transcription remains an open question. Here, we determined chromatin folding composition over several hundred kb around estrogen responsive genes human breast cancer cell lines following hormone stimulation. Modeling of 5C data 1.8 resolution was combined with quantitative 3D analysis multicolor...
<b>Introduction:</b> Determination of epithelial cells ciliary beat frequency (CBF) requires the introduction variational methods image analysis e.g. difference between two consecutive images (DI) or optical flow approach (OF) by correlation summing displacements (1). Aim study. To compare results obtained using DI versus OF. <b>Methods:</b> Human bronchial from 1 healthy donor (NHBE) and patient suffering COPD (DHBE) were purchased a commercial source. Epithelial differentiated for 21 days...
The crucial role of the major histocompatibility complex (MHC) for immune response to infectious diseases is well-known, but no information available on 3D nuclear organization this gene-dense region in cells, whereas architecture known play an essential genome function regulation. We analyzed spatial arrangement three MHC regions (class I, III and II) macrophages using 3D-FISH. Since presents differences humans pigs with, notably, presence centromere between class II pigs, analysis was...