A5053103180- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Sperm and Testicular Function
- RNA modifications and cancer
- DNA Repair Mechanisms
- Fungal and yeast genetics research
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Urologic and reproductive health conditions
- Liver Disease Diagnosis and Treatment
- Algal biology and biofuel production
- Adipose Tissue and Metabolism
- Advanced biosensing and bioanalysis techniques
- Biopolymer Synthesis and Applications
- Toxin Mechanisms and Immunotoxins
- Heat shock proteins research
- Drug Transport and Resistance Mechanisms
- Plant Disease Resistance and Genetics
- Mitochondrial Function and Pathology
- Muscle metabolism and nutrition
- Chromosomal and Genetic Variations
Centre for Cellular and Molecular Biology
2014-2023
Council of Scientific and Industrial Research
2014-2023
Government of Gujarat
2014
Banaras Hindu University
1970-2008
Tata Consultancy Services (India)
2006
University of California, San Diego
1997-1999
Institute of Medical Sciences
1984
Institute of Minerals and Materials Technology
1968-1983
National Cancer Institute
1983
National Institutes of Health
1983
Four novel bacterial strains were isolated from cryogenic tubes used to collect air samples at altitudes of 24, 28 and 41 km. The four strains, 24K(T), 28K(T), 41KF2a(T) 41KF2b(T), identified as members the genus Bacillus. Phylogenetic analysis based on 16S rRNA gene sequences indicated that three 28K(T) 41KF2a(T), are very similar one another (>98 % sequence similarity) show a similarity 98-99 with Bacillus licheniformis 98 sonorensis. DNA-DNA hybridization studies showed exhibit <70 each...
Three novel bacterial strains, PVAS-1T, B3W22T and B8W22T, were isolated from cryotubes used to collect air samples at altitudes of between 27 41 km. Based on phenotypic characteristics, chemotaxonomic features, DNA–DNA hybridization with the nearest phylogenetic neighbours analysis based partial 16S rRNA gene sequences (PVAS-1T, 1196 nt; B3W22T, 1541 1533 nt), three strains identified as representing species, names proposed are Janibacter hoylei sp. nov. (type strain PVAS-1T =MTCC 8307T...
Two pure cultures of bacteria isolated from soil samples collected in Schirmacher Oasis, Antarctica, conformed to the definition genus Sphingobacterium. They differed all known species Sphingobacterium being psychrotrophic. The G+C contents DNA two strains were found be 39.3 and 40.3 mol%, DNA-DNA hybridization studies indicated 7% homology with S. multivorum spiritivorum. name antarcticus sp. nov. is proposed for Antarctic strains. type strain 4BY (MTCC 675), it has been deposited Microbial...
Ten cultures of Pseudomonas spp. were established from soil samples collected in and around a lake Antarctica. Based on their morphology, biochemical physiological characteristics, moles percent G + C DNA, they identified as P. fluorescens, putida, syringae. This is the first report identification continental
To study nucleosome mobility and positioning, the R3 lac repressor was used with an adenosine triphosphate (ATP)–dependent chromatin assembly system to establish positioning of five nucleosomes, one located between two operators. When protein dissociated from DNA isopropyl β- d -thiogalactopyranoside, R3-induced positions remained unchanged for at least 60 minutes in absence ATP but rearranged within 15 presence ATP. These results suggest that nucleosomes are dynamic mobile rather than...
RNA polymerase (pol) III transcribes a unique class of genes with intra-genic promoters and high transcriptional activity. The major contributors to the pol transcriptome, tRNAs are found scattered on all chromosomes yeast. A prototype tDNA <150 bp length, is generally considered nucleosome-free while some III-transcribed have been shown nucleosome-positioning properties. Using resolution ChIP-chip ChIP-seq methods, we several features associated nucleosome profiles tRNA budding yeast, not...
Chromosomes architecture is viewed as a key component of gene regulation, but principles chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics longest chromosome Saccharomyces cerevisiae (XII). Chromosome XII carries ribosomal DNA (rDNA) that defines nucleolus, major hallmark nuclear organization. We determined intranuclear positions 15 loci distributed every ∼100 kb along chromosome, investigated their motion over...
FACT complex is involved in elongation and ensures fidelity the initiation step of transcription by RNA polymerase (pol) II. Histone variant H2A.Z found nucleosomes at 5′-end many genes. We report here H2A.Z-chaperone activity yeast on short, nucleosome-free, non-coding, pol III-transcribed tRNA On a prototype gene, SUP4, chromatin remodeler RSC regulate its through novel mechanisms, wherein two gene-flanking containing H2A.Z, play different roles. Nhp6, which helps load yFACT onto gene...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudies on the Structure of Skin Protein-bound Compounds Following Topical Application 1,2,5,6-Dibenzanthracene-9,10-C14. II. Nature 2-Phenylphenanthrene-3,2'-dicarboxylic Acid-Protein Bond1,2P. M. Bhargava and Charles HeidelbergerCite this: J. Am. Chem. Soc. 1956, 78, 15, 3671–3677Publication Date (Print):August 1, 1956Publication History Published online1 May 2002Published inissue 1 August...
The association of RNA polymerase III (Pol III)-transcribed genes with nucleoli seems to be an evolutionarily conserved property the spatial organization eukaryotic genomes. However, recent studies global chromosome architecture in budding yeast have challenged this view. We used live-cell imaging determine intranuclear positions 13 Pol III-transcribed genes. frequency nucleolus and nuclear periphery depends on linear genomic distance from tethering elements-centromeres or telomeres....
Seminalplasmin Caltrin Seminal plasma protein
The genes transcribed by RNA polymerase III (Pol III) generally have intragenic promoter elements. One of them, the yeast U6 snRNA (SNR6) gene is activated in vitro a positioned nucleosome between its box A and extragenic, downstream B separated approximately 200 bp. We demonstrate here that vivo chromatin structure region characterized presence an array nucleosomes, with only one them 5' end having regulatory role. present boxes does not move when repressed due to nutritional deprivation....
Impact of ultraviolet-B (UV-B) and Cd, applied individually in combination, measured terms oxygen-evolution, chlorophyll (Chl) protein contents, lipid peroxidation, enzymatic non-enzymatic antioxidants Anabaena doliolum, revealed a greater oxidative damage induced by UV-B than Cd. While superoxide dismutase (SOD) showed stimulation the activities catalase (CAT) glutathione reductase (GR) declined after treatment. Cd treatment, however, enhanced ascorbate peroxidase (APX) GR. CAT activity...