Abstract Gut bacterial microbiome dysbiosis in type 2 Diabetes Mellitus (T2DM) has been reported, but such an association with Diabetic Retinopathy (DR) is not known. We explored possible link between gut and DR. Using fecal samples of healthy controls (HC) people T2DM with/without DR, communities were analysed using 16S rRNA gene sequencing data QIIME R software. Dysbiosis the microbiomes, at phyla genera level, was observed DR compared to HC. People exhibited greater discrimination from...

Four novel bacterial strains were isolated from cryogenic tubes used to collect air samples at altitudes of 24, 28 and 41 km. The four strains, 24K(T), 28K(T), 41KF2a(T) 41KF2b(T), identified as members the genus Bacillus. Phylogenetic analysis based on 16S rRNA gene sequences indicated that three 28K(T) 41KF2a(T), are very similar one another (>98 % sequence similarity) show a similarity 98-99 with Bacillus licheniformis 98 sonorensis. DNA-DNA hybridization studies showed exhibit <70 each...

Three novel bacterial strains, PVAS-1T, B3W22T and B8W22T, were isolated from cryotubes used to collect air samples at altitudes of between 27 41 km. Based on phenotypic characteristics, chemotaxonomic features, DNA–DNA hybridization with the nearest phylogenetic neighbours analysis based partial 16S rRNA gene sequences (PVAS-1T, 1196 nt; B3W22T, 1541 1533 nt), three strains identified as representing species, names proposed are Janibacter hoylei sp. nov. (type strain PVAS-1T =MTCC 8307T...

With a view to understand the molecular basis of sperm motility, we have tried establish human proteome by two-dimensional PAGE MALDI MS/MS analysis. We report identification 75 different proteins in spermatozoa. Comparative analysis was carried out for asthenozoospermic and normozoospermic patients motility. Analysis revealed eight (including one unidentified) with altered intensity between groups. Differential distributed into three functional groups: 'energy metabolism' (triose-phosphate...

Abstract The proof-of-concept, study to investigate the presence of microorganisms in presumed infectious endophthalmitis using Next generation sequencing (NGS) was carried out vitreous biopsies from 34 patients with endophthalmitis, and thirty undergoing surgery for non-infectious retinal disorders as controls. Following DNA extraction Qiagen mini kit PCR amplification V3–V4 regions bacterial 16S rRNA ITS 2 region fungus, they samples were sequenced on an Illumina HiSeq 2500 Machine. Paired...

Studies have documented dysbiosis in the gut mycobiome people with Type 2 diabetes mellitus (T2DM). However, it is not known whether of T2DM patients would be reflected diabetic retinopathy (DR) and if so, observed similar DR. Gut mycobiomes were generated from healthy controls (HC), DR through Illumina sequencing ITS2 region. Data analysed using QIIME R software. Dysbiotic changes compared to HC at phyla genera level. Mycobiomes HC, could discriminated by heat map analysis, Beta diversity...

CMS 19YT, a psychrophilic bacterium, was isolated from cyanobacterial mat sample pond in Antarctica and characterized taxonomically. The bacterium aerobic, gram-positive, non-spore-forming, non-motile, exhibited rod-coccus growth cycle produced yellow pigment that insoluble water but soluble methanol. No factors were required it able to grow between 5 30 degrees C, pH 6 9 tolerated up 11.5% NaCl. cell wall peptidoglycan Lys-Thr-Ala3 (the A3alpha variant) the major menaquinone MK-9(H2). G+C...

Two pure cultures of bacteria isolated from soil samples collected in Schirmacher Oasis, Antarctica, conformed to the definition genus Sphingobacterium. They differed all known species Sphingobacterium being psychrotrophic. The G+C contents DNA two strains were found be 39.3 and 40.3 mol%, DNA-DNA hybridization studies indicated 7% homology with S. multivorum spiritivorum. name antarcticus sp. nov. is proposed for Antarctic strains. type strain 4BY (MTCC 675), it has been deposited Microbial...

Thirty-one bacteria that belonged to the genus Pseudomonas were isolated from cyanobacterial mat samples collected various water bodies in Antarctica. All 31 isolates psychrophilic; they could be divided into three groups, based on their protein profiles. Representative strains of each namely CMS 35 T , 38 and 64 studied detail. Based 16S rRNA gene sequence analysis, it was established related closely fluorescens group. Phenotypic chemotaxonomic characteristics further confirmed affiliation...

Bacterial diversity in sub-Antarctic seawater, collected off Ushuaia, Argentina, was examined using a culture independent approach. The composition of the 16S rRNA gene libraries from seawater and contaminated with water soluble fraction crude oil statistically different (P value 0.001). In both libraries, clones representing Alphaproteobacteria, Gammaproteobacteria, Cytophaga-Flavobacterium-Bacteroidetes group unculturable bacteria were dominant. Clones associated genera Roseobacter,...

Ten cultures of Pseudomonas spp. were established from soil samples collected in and around a lake Antarctica. Based on their morphology, biochemical physiological characteristics, moles percent G + C DNA, they identified as P. fluorescens, putida, syringae. This is the first report identification continental

Three strains of Sphingomonas paucimobilis , B90A, UT26 and Sp+, isolated from different geographical locations, were found to degrade hexachlorocyclohexane. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these do not fall in a clade includes the type strain, ATCC 29837 T but form coherent cluster with [ ] chungbukensis IMSNU 11152 followed by Sphingobium chlorophenolicum 33790 . The three showed low DNA–DNA relatedness values (8–25 %), (10–17 (23–54 %) xenophaga DSM...

Purpose: To report the molecular and microbiological diagnosis clinical profile of 13 patients with Pythium insidiosum keratitis. Methods: Phase 1 study consisted DNA sequencing ITS region rDNA 162 stocked morphologically unidentified nonconsecutive fungal isolates from corneal scraping keratitis (2010–2012). Blast phylogenetic analyses sequences showed 9 to be P. insidiosum. A retrospective review archived photographs colony direct microscopy scrapings records cases were performed. 2 began...

Dysbiosis in the gut microbiome has been implicated several diseases including auto-immune diseases, inflammatory cancers and mental disorders. Keratitis is an disease of eye significantly contributing to corneal blindness developing world. It would be worthwhile investigate possibility dysbiosis being associated with Keratitis. Here, we have analyzed fungal bacterial populations stool samples through high-throughput sequencing ITS2 region for fungi V3-V4 16S rRNA gene bacteria healthy...

Purpose: To enumerate the ocular surface fungal microbiome of healthy human eyes by using next-generation sequencing (NGS). Methods: Tarsal and fornix conjunctiva from lower upper lids both individuals were swabbed in duplicate separately. A total 34 samples collected 17 individuals, which used for generation microbiomes NGS. Twenty-four swabs detection culturable fungi conventional cultivable method. Microbiome involved DNA extraction, internal transcribed spacer 2 (ITS2) amplification,...

Strain CMS 76orT, an orange-pigmented bacterium, was isolated from a cyanobacterial mat sample pond located in McMurdo Dry Valley, Antarctica. On the basis of chemotaxonomic and phylogenetic properties, strain 76orT identified as member genus Kocuria. It exhibited 16S rDNA similarity 99.8% DNA-DNA 71% with Kocuria rosea (ATCC 186T). Phenotypic traits confirmed that 78orT K. were well differentiated. Furthermore, could be differentiated other reported species Kocuria, namely kristinae...

The psychrotrophic, dimorphic yeast Candida humicola, isolated from Antarctic soil, secretes an acidic protease into the medium. secretion of this by C. humicola was found to be dependent on composition In YPD or nitrogen base medium containing either amino acids ammonium sulfate as source, activity in low (basal level). However, when depleted and supplemented with proteins, enzyme increased. greater during exponential growth at temperatures than higher temperatures. purified had a molecular...

Strain Sd/3 T (=MTCC 4374 =DSM 15820 ), an arsenic-resistant bacterium, was isolated from a sand sample obtained arsenic-contaminated aquifer in Chakdah district West Bengal, India (23° 3′ N 88° 35′ E). The bacterium Gram-positive, rod-shaped, non-motile, endospore-forming and yellowish-orange pigmented. It possessed all the characteristics that conform to genus Bacillus , such as it had A 4 β murein type ( l -orn- d -Asp) peptidoglycan variant, MK-7 major menaquinone iso-C15 : 0 anteiso-C15...

An arsenic- and radiation-resistant bacterium, strain Wt/1a(T), was isolated from water an arsenic-contaminated aquifer located in the Chakdah district of West Bengal, India. The bacterium stains Gram-negative is rod-shaped, non-motile, non-sporulating red-pigmented. Cell-wall peptidoglycan contains ornithine as diamino acid, MK-8 major menaquinone, C(15 : 1) C(16 are fatty acids DNA G+C content organism 65.8 mol%. Based on these phenotypic chemotaxonomic characteristics, Wt/1a(T) identified...