A5033614909- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Nanopore and Nanochannel Transport Studies
- HIV Research and Treatment
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- SARS-CoV-2 detection and testing
- Innovative Microfluidic and Catalytic Techniques Innovation
- Force Microscopy Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Blood properties and coagulation
- Analytical Chemistry and Chromatography
- Erythrocyte Function and Pathophysiology
- Cellular Mechanics and Interactions
- Rheology and Fluid Dynamics Studies
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- interferon and immune responses
- Computational Drug Discovery Methods
- Zeolite Catalysis and Synthesis
- Advanced biosensing and bioanalysis techniques
- Graph theory and applications
- Extracellular vesicles in disease
- Analytical Chemistry and Sensors
- Genetic Neurodegenerative Diseases
Institut de Recherche en Infectiologie de Montpellier
2019-2025
Université de Montpellier
2019-2025
Centre National de la Recherche Scientifique
2012-2025
Laboratoire d'Analyse et d'Architecture des Systèmes
2015-2019
Université de Toulouse
2017-2019
Inserm
2012-2013
Laboratoire Adhésion et inflammation
2012
Aix-Marseille Université
2012
Laboratoire d’Electrochimie et de Physico-chimie des Matériaux et des Interfaces
2010
Institut polytechnique de Grenoble
2010
At the cellular scale, blood fluidity and mass transport depend on dynamics of red cells in flow, specifically their deformation orientation. These are governed by rheological properties, such as internal viscosity cytoskeleton elasticity. In diseases which cell rheology is altered genetically or parasitic invasion changes microenvironment, flow may be severely impaired. The nonlinear interplay between generate complex dynamics, remain largely unexplored experimentally. Under simple shear...
Abstract DNA folding and dynamics along with major nuclear functions are determined by chromosome structural properties, which remain, thus far, elusive in vivo. Here, we combine polymer modeling single particle tracking experiments to determine the physico-chemical parameters of chromatin vitro living yeast. We find that motion reconstituted fibers can be recapitulated Rouse model using mechanical nucleosome arrays deduced from simulations. Conversely, report shows some inconsistencies...
Retroviruses carry a genomic intron-containing RNA with long structured 5'-untranslated region, which acts either as genome encapsidated in the viral progeny or an mRNA encoding key structural protein, Gag. We developed single-molecule microscopy approach to simultaneously visualize and nascent Gag protein during translation directly cell. found that minority of molecules serve they are translated fast efficient process. Surprisingly, polysomes were also observed at cell periphery,...
Cellular mRNAs are exported from the nucleus as fully spliced RNAs. Proofreading mechanisms eliminate unprocessed and irregular pre-mRNAs to control quality of gene expression. Retroviruses need export partially unspliced full-length RNAs cytoplasm where they serve templates for protein synthesis and/or encapsidated RNA in progeny viruses. Genetically complex retroviruses such HIV-1 use Rev-equivalent proteins intron-retaining using cellular CRM1-driven nuclear machinery. By contrast,...
We present µLAS, a lab-on-chip system that concentrates, separates, and detects DNA fragments in single module. µLAS speeds up size analysis minutes using femtomolar amounts of amplified DNA. Here we tested the relevance for sizing expanded trinucleotide repeats, which cause over 20 different neurological neuromuscular disorders. Because length repeats correlates with severity diseases, it is crucial to be able repeat tract accurately efficiently. Expanded are however genetically unstable...
DNA separation and analysis have advanced over recent years, benefiting from microfluidic systems that reduce sample volumes costs, essential for sequencing disease identification in body fluids. We recently developed the μLAS technology enables separation, concentration, of nucleic acids with high sensitivity. The combines a hydrodynamic flow actuation an opposite electrophoretic force viscoelastic polymer solutions. Combining hydrodynamics first principles statistical mechanics, we...
The dynamics of single DNA molecules conveyed in a viscoelastic fluid flow with an opposing electrophoretic force are investigated by fluorescence microscopy. For balanced hydrodynamic and forces, is confined near the walls much smaller elongation than bulk shear flows. Furthermore, we observe that extension characterized intermittent fluctuations, characteristic time scale which depends on velocity. A model based Rouse explains contraction molecule coupling monomer motion transverse...
This paper presents experimental results about transport of dilute suspensions nano-objects in silicon-glass micrometric and sub-micrometric channels. Two kinds objects are used: solid, rigid latex beads spherical capsule-shaped, soft polymersomes. They tracked using fluorescence microscopy. Three parameters studied: confinement (ratio between particle diameter channel depth), Brownian diffusion nature. The aim this work is to understand how these different affect the narrow channels...
Nanopores combined with optical approaches can be used to detect viral particles. In this work, we demonstrate the ability of hydrodynamical driving and sensing identify quantify particles in a biological sample. We have developed simple rapid method which requires only fluorescent labeling therefore applied wide range virus type. The system operates real time at single particle level while providing low error on concentration (4%) limit detection 105 particles/mL for an acquisition 60 s...
Abstract Chromosome mechanical properties determine DNA folding and dynamics, underlie all major nuclear functions. Here we combine modeling real-time motion tracking experiments to infer the physical parameters describing chromatin fibers. In vitro, of nucleosome arrays can be accurately modeled by assuming a Kuhn length 35-55 nm. vivo , amplitude chromosome fluctuations is drastically reduced, depends on transcription. Transcription activation increases dynamics only if it involves gene...
Abstract Viruses have remarkable physical properties and complex interactions with their environment. However, aggregation in confined spaces remains unexplored, although this phenomenon is of paramount importance for understanding viral infectivity. Using hydrodynamical driving optical detection, we developed a method to detect the transport single virus real time through synthetic nanopores. We unveiled jamming specifically associated confinement under flow. showed that particles...
Abstract An alternative method for solving homogenous and non‐homogenous differential equation systems used in chemical kinetics pharmacokinetics on the basis of flow graph principles has been proposed. The classical these with graphs involves employment Laplace transform before depicting a inverse after using Mason's rules. A short description algebra presented. One model very often encountered was solved by both methods order to make comparison. Our proposed is simpler more direct,...
Abstract For see ChemInform in Full Text.