A5082973457- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Nanopore and Nanochannel Transport Studies
- Molecular Biology Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Cancer Genomics and Diagnostics
- Electrochemical Analysis and Applications
- Semiconductor materials and devices
- Fungal and yeast genetics research
- Granular flow and fluidized beds
- Electrohydrodynamics and Fluid Dynamics
- Rheology and Fluid Dynamics Studies
- Analytical Chemistry and Sensors
- Genetic Neurodegenerative Diseases
- RNA and protein synthesis mechanisms
- Material Dynamics and Properties
- Single-cell and spatial transcriptomics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Electrowetting and Microfluidic Technologies
Roche (France)
2020-2021
Laboratoire d'Analyse et d'Architecture des Systèmes
2017-2021
Centre National de la Recherche Scientifique
2017-2021
Université de Toulouse
2017-2019
We present µLAS, a lab-on-chip system that concentrates, separates, and detects DNA fragments in single module. µLAS speeds up size analysis minutes using femtomolar amounts of amplified DNA. Here we tested the relevance for sizing expanded trinucleotide repeats, which cause over 20 different neurological neuromuscular disorders. Because length repeats correlates with severity diseases, it is crucial to be able repeat tract accurately efficiently. Expanded are however genetically unstable...
DNA separation and analysis have advanced over recent years, benefiting from microfluidic systems that reduce sample volumes costs, essential for sequencing disease identification in body fluids. We recently developed the μLAS technology enables separation, concentration, of nucleic acids with high sensitivity. The combines a hydrodynamic flow actuation an opposite electrophoretic force viscoelastic polymer solutions. Combining hydrodynamics first principles statistical mechanics, we...
The dynamics of single DNA molecules conveyed in a viscoelastic fluid flow with an opposing electrophoretic force are investigated by fluorescence microscopy. For balanced hydrodynamic and forces, is confined near the walls much smaller elongation than bulk shear flows. Furthermore, we observe that extension characterized intermittent fluctuations, characteristic time scale which depends on velocity. A model based Rouse explains contraction molecule coupling monomer motion transverse...
We report a detailed study of band broadening in microfluidic chip for DNA separation by means electrohydrodynamic viscoelatic migration.
Circulating cell-free DNA (cfDNA) is a powerful cancer biomarker for establishing targeted therapies or monitoring patients' treatment. However, current cfDNA characterization severely limited by its low concentration, requiring the extensive use of amplification techniques. Here we report that μLAS technology allows us to quantitatively characterize size distribution purified in few minutes, even when concentration as 1 pg/μL. Moreover, show profiles can be directly measured blood plasma...