A5061253585- Microtubule and mitosis dynamics
- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- RNA Research and Splicing
- Nuclear Structure and Function
- Monoclonal and Polyclonal Antibodies Research
- Synthesis and Biological Evaluation
- Bacteriophages and microbial interactions
- Plant nutrient uptake and metabolism
- Supramolecular Self-Assembly in Materials
- Molecular Biology Techniques and Applications
- Radiopharmaceutical Chemistry and Applications
European Molecular Biology Laboratory
2021-2023
DKFZ-ZMBH Alliance
2023
German Cancer Research Center
2023
Heidelberg University
2021
European Molecular Biology Organization
2019
European Molecular Biology Laboratory
2014-2017
Human chromosomes are captured along microtubule walls (lateral attachment) and then tethered to microtubule-ends (end-on through a multi-step end-on conversion process. Upstream regulators that orchestrate this remarkable change in the plane of kinetochore-microtubule attachment human cells not known. By tracking kinetochore movements using markers specific status, we reveal spatially defined role for Aurora-B kinase retarding To understand how activity is counteracted, compare roles two...
Proper chromosome segregation is crucial for cell division. In eukaryotes, this achieved by the kinetochore, an evolutionarily conserved multiprotein complex that physically links DNA to spindle microtubules and takes active role in monitoring correcting erroneous spindle–chromosome attachments. Our mechanistic understanding of these functions how they ensure error-free outcome mitosis still limited, partly because we lack a complete kinetochore structure cell. study, use single-molecule...
Abstract Quantitative fluorescence and superresolution microscopy are often limited by insufficient data quality or artifacts. In this context, it is essential to have biologically relevant control samples benchmark optimize the of microscopes, labels imaging conditions. Here we exploit stereotypic arrangement proteins in nuclear pore complex as situ reference structures characterize performance a variety modalities. We created four genome edited cell lines which endogenously labeled...
Abstract Proper chromosome segregation is crucial for cell division. In eukaryotes, this achieved by the kinetochore, an evolutionarily conserved multi-protein complex that physically links DNA to spindle microtubules and takes active role in monitoring correcting erroneous spindle-chromosome attachments. Our mechanistic understanding of these functions how they ensure error-free outcome mitosis still limited, partly because we lack a comprehensive kinetochore structure cell. study, use...