A5101949789- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Bacteriophages and microbial interactions
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Kruppel-like factors research
- Circular RNAs in diseases
- RNA modifications and cancer
- Plasmonic and Surface Plasmon Research
- Cancer-related gene regulation
- Cancer Genomics and Diagnostics
- Infant Nutrition and Health
- Biosensors and Analytical Detection
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Protein Degradation and Inhibitors
- Nanocluster Synthesis and Applications
- Lipid Membrane Structure and Behavior
- DNA and Biological Computing
- Medicinal Plants and Bioactive Compounds
- Radiation Effects in Electronics
- Nicotinic Acetylcholine Receptors Study
- Cancer Risks and Factors
- Circadian rhythm and melatonin
Yale University
2019-2025
China Electronic Product Reliability and Environmental Test Institute
2024
Hefei Institutes of Physical Science
2024
Anhui University
2024
Zhengzhou University
2024
Purdue University West Lafayette
2017-2023
University of South China
2010-2023
Huaian First People’s Hospital
2023
Nanjing Medical University
2018-2023
Central South University
2014-2021
Quasicrystals are a class of materials with long-range order but no translational periodicity. Though many quasicrystals have been discovered, rational design and engineering remain great challenge. Herein, we developed strategy to assemble two-dimensional (2D), dodecagonal from branched DNA nanomotifs. The key our is balance the rigidity flexibility motifs, which controlled by introduction interbranch "bridges". By fine-tuning experimental conditions, able predictably produce either 2D or...
Engineered 3D DNA crystals are promising scaffolds for bottom-up construction of three-dimensional, macroscopic devices from the molecular level. Nevertheless, this has been hindered by highly constrained conditions to be stable. Here we report a method prepare robust postassembly ligation remove constraint. Specifically, sticky ends at crystal contacts were enzymatically ligated, and covalent bonds significantly enhanced stability, e.g., being stable 65 °C. This also enabled fabrication...
The DNA-origami technique has enabled the engineering of transmembrane nanopores with programmable size and functionality, showing promise in building biosensors synthetic cells. However, it remains challenging to build large (>10 nm), functionalizable that spontaneously perforate lipid membranes. Here, we take advantage pneumolysin (PLY), a bacterial toxin potently forms wide ring-like channels on cell membranes, construct hybrid DNA-protein nanopores. This PLY-DNA-origami complex, which...
Abstract Background Opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) has been demonstrated to play vital roles in development and progression of tumors such as gastric cancer (GC). However, the detailed molecular mechanism OIP5-AS1 not completely elucidated. Our study aimed investigate role epigenetic regulation GC. Methods expression GC tissues was detected by RT-qPCR. Loss- gain-of-function experiments were conducted assess biological function vitro vivo. The interaction with...
Herein, we report the development of a new "smart" radioactive probe (i.e., 1) which can undergo furin-controlled condensation and self-assembly nanoparticles 1-NPs) in tumor cells its application for enhanced microPET imaging tumors nude mice co-injected with cold analog 1-Cold). Furin-controlled 1-Cold 1-Cold-NPs) vitro were validated characterized HPLC, mass spectra, SEM, TEM analyses. Cell uptake studies showed that both 1 have good cell permeability. images 1-Cold-treated MDA-MB-468...
Responsive self-assembly is a general process in biological systems and highly desired engineered systems. DNA nanostructures provide versatile molecular platform for studying such responsive self-assembly. Various triggers have been explored nanostructures. However, each trigger requires unique mechanism its response. This situation brings great challenge to engineer the responsiveness. Herein, we propose an aptamer-based, allosteric The aptamer–ligand binding causes motif change...
<b><i>Summary</i></b><b><i>Background: </i></b>This study aims to characterize the function of downregulated MicroRNA miR-205 in renal cell carcinoma (RCC), and show how downstream zinc finger E-box-binding homeobox 2 (ZEB2) is negatively regulated by miR-205. <b><i>Materials Methods: </i></b>The expression was detected RCC adjacent non-tumor tissues using real-time polymerase chain reaction (PCR). The ZEB2 lines PCR....
We report a nanopatterning strategy in which self-assembled DNA nanostructures serve as structural templates. In previous work, ordering of NPs primarily relied on specific recognition, e.g., DNA–DNA hybridization. Only few cases have been reported nonspecific adsorption. Unfortunately, these studies were limited by the integrity and homogeneity templates variety patterned nanoparticles (NPs). Herein, we developed general method to pattern various NPs. The adsorb onto substrate via...
DNA self-assembly provides a powerful approach for preparation of nanostructures. It is often studied in bulk solution and involves only DNA-DNA interactions. When confined to surfaces, DNA-surface interactions become an additional, important factor self-assembly. However, the way which influence not well studied. In this study, we showed that weak could be stabilized by allow large nanostructures form. addition, assembly can conducted isothermally at room temperature as little 5 seconds.
Acute lung injury is characterized by alveolar vascular barrier injury, and protein-rich pulmonary oedema. Alveolar fluid clearance closely related to the prognosis of patients with acute injury. Melatonin has been shown have a protective effect on multiple organ induced sepsis. In this study we investigated melatonin (AFC) explored its potential mechanisms in sepsis-induced The cecal ligation puncture was adopted establish mouse sepsis model. Morphological changes tissues hematoxylin...
Membrane dynamics in living organisms can arise from proteins adhering to, assembling on, and exerting force on cell membranes. Programmable synthetic materials, such as self-assembled DNA nanostructures, offer the capability to drive membrane-remodeling events that resemble protein-mediated but with user-defined outcomes. An illustrative example is tubular deformation of liposomes by nanostructures purposely designed shapes, surface modifications, self-assembling properties. However,...
Customizable nanostructures built through the DNA-origami technique hold tremendous promise in nanomaterial fabrication and biotechnology. Despite cutting-edge tools for design preparation, it remains challenging to separate structural components of an architecture from-thus held together by-a continuous scaffold strand, which turn limits modularity function devices. To address this challenge, here we present enzymatic method clean up reconfigure structures. We target single-stranded (ss)...
microRNA (miR)‑195 acts as a suppressor in multiple types of malignant tumors, including bladder cancer; however, the detailed function miR‑195 cancer remains largely unknown. The aim present study was to investigate role regulation cell proliferation and determine whether division control protein 42 homolog (Cdc42)/signal transducer activator transcription‑3 (STAT3) signaling downstream effector cells. Reverse transcription‑quantitative polymerase chain reaction used expression levels...
OPA-interacting protein 5 antisense transcript 1 (OIP5-AS1) plays an important regulatory role in various types of cancers.However, the functional and mechanisms OIP5-AS1 gastric cancer (GC) remain largely unknown.In this study, we found that expression was increased GC tissues compared with adjacent non-cancerous tissues, which significantly associated shorter overall survival time patients.In addition, also cell lines including NCI-N87, MKN-45, BGC-823 SGC-7901, when normal epithelial line...