A5100423008- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Bacteriophages and microbial interactions
- Neuroscience and Neuropharmacology Research
- Molecular Junctions and Nanostructures
- Biosensors and Analytical Detection
- Renal and related cancers
- Estrogen and related hormone effects
- Genetics and Neurodevelopmental Disorders
- Lipid Membrane Structure and Behavior
- Birth, Development, and Health
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Advanced Fluorescence Microscopy Techniques
- Plasmonic and Surface Plasmon Research
- RNA modifications and cancer
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Genetic Associations and Epidemiology
- Tryptophan and brain disorders
- Bipolar Disorder and Treatment
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Biotin and Related Studies
Central Hospital of Wuhan
2022-2025
General Hospital of Central Theater Command
2022-2025
Sichuan University
2024-2025
Wenzhou University
2025
Ministry of Natural Resources
2025
Huazhong Agricultural University
2024-2025
University of Wisconsin–Madison
2020-2024
Shanghai Jiao Tong University
2010-2024
Howard Hughes Medical Institute
2020-2024
PTC Therapeutics (United States)
2024
<h3>Background</h3> CD8<sup>+</sup> T cells differentiate into exhausted status within tumors, including hepatocellular carcinoma (HCC), which constitutes a solid barrier to effective anti-tumor immunity. A detailed characterization of and their prognostic value in HCC is lacking. <h3>Methods</h3> We collected fresh tumor tissues with adjacent non-tumor liver blood specimens 56 patients, as well archived samples from two independent cohorts patients (<i>n</i> = 358 <i>n</i> 254), who...
Self-assembled DNA origami nanostructures have shown great promise for bottom-up construction of complex objects with nanoscale addressability. Here we show that origami-based 1D nanoribbons and nanotubes are one-pot assembled controllable sizes addressability high speed (within only 10–20 min), exhibiting extraordinarily cooperativity is often observed in assembly natural molecular machines cells (e.g. ribosome). By exploiting the specificity DNA-based self-assembly, can precisely anchor...
Mesenchymal stem cells (MSCs) can donate mitochondria and rescue anthracycline-induced cardiomyocyte (CM) damage, although the underlying mechanisms remain elusive. We determined that superior efficiency of mitochondrial transfer by human induced-pluripotent-stem-cell-derived MSCs (iPSC-MSCs) compared with bone marrow-derived (BM-MSCs) is due to high expression intrinsic Rho GTPase 1 (MIRO1). Further, a higher level TNFαIP2 expression, iPSC-MSCs are more responsive tumor necrosis factor...
Small-conductance Ca 2+ -activated K + channels (SK channels, channels) have been reported in excitable cells, where they aid integrating changes intracellular with membrane potential. We recently for the first time functional existence of SK2 (K 2.2) human and mouse cardiac myocytes. Here, we report cloning SK1 2.1) SK3 2.3) from atria ventricles using RT-PCR. Full-length transcripts their variants were detected both channels. Variants channel (mSK1) differ mainly COOH-terminal structure,...
Innate immunity is an indispensable arm of tumor immune surveillance, and the liver organ with a predominance innate immunity, where mucosal-associated invariant T (MAIT) cells are enriched. However, little known about phenotype, functions, immunomodulatory role MAIT in hepatocellular carcinoma (HCC).Experimental Design: The distribution, function patients HCC were evaluated by both flow cytometry (FCM) vitro bioassays. Transcriptomic analysis was also performed. Prognostic significance...
Chemokines play a key role in orchestrating the recruitment and positioning of myeloid cells within tumor microenvironment. However, tropism regulation functions these hepatocellular carcinoma (HCC) are not completely understood. Herein, by scrutinizing expression all chemokines HCC cell lines tissues, we found that CCL15 was most abundantly expressed chemokine human HCC. Further analyses showed regulated genetic, epigenetic, microenvironmental factors, negatively correlated with patient...
Abstract Middle East respiratory syndrome coronavirus (MERS-CoV) belongs to beta group of and was first discovered in 2012. MERS-CoV can infect multiple host species cause severe diseases human. We conducted a series phylogenetic bioinformatic analyses study the evolution dynamics among different with genomic data. Our show: 1) 28 potential recombinant sequences were detected they be classified into seven types; 2) The spike (S) protein under strong positive selection when transmitted from...
A major goal of nanotechnology and bioengineering is to build artificial nanomachines capable generating specific membrane curvatures on demand. Inspired by natural membrane-deforming proteins, we designed DNA-origami curls that polymerize into nanosprings show their efficacy in vesicle deformation. DNA-coated tubules emerge from spherical vesicles when polymerization or high membrane-surface coverage occurs. Unlike many previous methods, the DNA self-assembly-mediated tubulation eliminates...
Congenital nephron number varies five-fold among normal humans, and individuals at the lower end of this range may have an increased lifetime risk for essential hypertension or renal insufficiency; however, mechanisms that determine are unknown. This study tested hypothesis common hypomorphic variants RET gene, which encodes a tyrosine kinase receptor critical branching morphogenesis, might account subtle hypoplasia in some newborns. A single-nucleotide polymorphism (rs1800860 G/A) was...
A DNA-origami chip platform for target-labeling-free single-nucleotide polymorphism (SNP) genotyping is presented. DNA capture probes are anchored on an asymmetric map-shaped and a “toehold”-mediated strand-displacement reaction employed to endow single-base-mismatch differentiation specificity. This design leads reliable potentially universal means SNP genotyping.
The DNA origami approach enables the construction of complex objects from strands. A fundamental understanding kinetics and thermodynamics assembly is extremely important for building large structures with multifunctionality. Here both experimental theoretical studies melting were carried out in order to reveal reversible association/disassociation process. Furthermore, by careful control temperature cycling via situ thermally controlled atomic force microscopy, self-assembly process a...