A5025133234- Mesenchymal stem cell research
- Cardiac Fibrosis and Remodeling
- Tissue Engineering and Regenerative Medicine
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Signaling Pathways in Disease
- Epigenetics and DNA Methylation
- Viral Infectious Diseases and Gene Expression in Insects
- Extracellular vesicles in disease
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Cancer-related gene regulation
- RNA modifications and cancer
- Cardiac Ischemia and Reperfusion
- Cancer, Hypoxia, and Metabolism
- Cardiac Structural Anomalies and Repair
- Immune Cell Function and Interaction
- Macrophage Migration Inhibitory Factor
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Galectins and Cancer Biology
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Aortic aneurysm repair treatments
- Endoplasmic Reticulum Stress and Disease
Shanghai East Hospital
2016-2025
Southern Medical University
2019-2025
Tongji University
2021-2024
Peking University
2022-2024
Peking University International Hospital
2022-2024
Nanjing University of Chinese Medicine
2024
Cambridge University Press
2023
University of Hong Kong
2012-2023
New York University Press
2023
Central China Normal University
2018-2022
Transplantation of mesenchymal stem cells (MSCs) holds great promise in the repair cigarette smoke (CS)-induced lung damage chronic obstructive pulmonary disease (COPD). Because CS leads to mitochondrial dysfunction, we aimed investigate potential benefit transfer from human-induced pluripotent cell-derived MSCs (iPSC-MSCs) CS-exposed airway epithelial vitro and vivo. Rats were exposed 4% for 1 hour daily 56 days. At Days 29 and, human iPSC-MSCs or adult bone marrow-derived (BM-MSCs)...
Mesenchymal stem cells (MSCs) can donate mitochondria and rescue anthracycline-induced cardiomyocyte (CM) damage, although the underlying mechanisms remain elusive. We determined that superior efficiency of mitochondrial transfer by human induced-pluripotent-stem-cell-derived MSCs (iPSC-MSCs) compared with bone marrow-derived (BM-MSCs) is due to high expression intrinsic Rho GTPase 1 (MIRO1). Further, a higher level TNFαIP2 expression, iPSC-MSCs are more responsive tumor necrosis factor...
Abstract Extracellular vesicles (EVs) curb important biological functions. We previously disclosed that ischemia‐reperfusion (IR) induces increased release of EVs (IR‐EVs) in the heart. However, role IR‐EVs IR pathological process remains poorly understood. Here we found adoptive transfer aggravated induced heart injury, and EV inhibition by GW4869 reduced injury. Our vivo vitro investigations substantiated facilitated M1‐like polarization macrophages with expression proinflammatory...
Accumulating evidence has shown that mesenchymal stem cell (MSC)-derived exosomes (exo) mediate cardiac repair following myocardial infarction (MI). Macrophage migration inhibitory factor (MIF), a proinflammatory cytokine, plays critical role in regulating homeostasis. This study aimed to investigate the cardioprotective effects of exo secreted from bone marrow-MSCs (BM-MSCs) overexpressing MIF rat model MI. plasmid was transducted BM-MSCs. Exo were isolated supernatants BM-MSCs and...
Abstract Osteosarcoma, an aggressive malignant cancer, has a high lung metastasis rate and lacks therapeutic target. Here, we reported that chromobox homolog 4 (CBX4) was overexpressed in osteosarcoma cell lines tissues. CBX4 promoted by transcriptionally up-regulating Runx2 via the recruitment of GCN5 to promoter. The phosphorylation at T437 casein kinase 1α (CK1α) facilitated its ubiquitination both K178 K280 subsequent degradation CHIP, this could be reduced TNFα. Consistently, CK1α...
Abstract Transplantation of bone marrow mesenchymal stem cells (BM-MSCs) can protect cardiomyocytes against anthracycline-induced cardiomyopathy (AIC) through paracrine effects. Nonetheless the effects human induced pluripotent cell-derived MSCs (iPSC-MSCs) on AIC are poorly understood. In vitro studies reveal that doxorubicin (Dox)-induced reactive oxidative stress (ROS) generation and cell apoptosis in neonatal rat (NRCMs) significantly reduced when treated with conditioned medium...
Paracrine effect is the major mechanism that underlies mesenchymal stem cells (MSC)-based therapy. This study aimed to examine how Rap1, telomeric repeat-binding factor 2-interacting protein 1 (Terf2IP), which a novel modulator involved in nuclear factor-kappaB (NF-κB) pathway, regulates paracrine effects of MSC-mediated heart repair following infarction. NF-κB activity stromal was increased by Rap1 as measured pNF-κB-luciferase reporter activity, and this abolished IkB-dominant-negative...
The age-related functional exhaustion limits potential efficacy of mesenchymal stem cells (MSC) in treating cardiovascular disease. Therefore, rejuvenation aged MSC the elderly population is great interest. We have previously reported that Erb-B2 receptor tyrosine kinase 4 (ERBB4) plays a critical role regulating survival under hypoxia. aim this study was to investigate whether ERBB4 rejuvenates and how enhances therapeutic myocardial infarction (MI). Compared with vector (aged-MSC),...
Abstract Aging impairs the functions of human mesenchymal stem cells (MSCs), thereby severely reducing their beneficial effects on myocardial infarction (MI). MicroRNAs (miRNAs) play crucial roles in regulating senescence MSCs; however, underlying mechanisms remain unclear. Here, we investigated significance miR‐155‐5p MSC and whether inhibition could rejuvenate aged MSCs (AMSCs) to enhance therapeutic efficacy for MI. Young (YMSCs) AMSCs were isolated from young donors, respectively. The...
Mesenchymal stem cell- (MSC-) based therapy is a novel strategy in regenerative medicine. The functional and capacities of MSCs decline with senescence. Nonetheless, the potential mechanisms that underlie their senescence are not fully understood. This study was aimed at exploring fibroblast growth factor 21 (FGF21) regulation MSC determined by senescence-associated β -galactosidase (SA- -gal) staining. morphology level mitochondrial reactive oxygen species (ROS) were assessed MitoTracker...
Mesenchymal stem cell (MSC)-based therapy has emerged as a novel strategy to treat many degenerative diseases. Accumulating evidence shows that the function of MSCs declines with age, thus limiting their regenerative capacity. Nonetheless, underlying mechanisms control MSC ageing are not well understood. We show compared bone marrow-MSCs (BM-MSCs) isolated from young and aged samples, NADH dehydrogenase (ubiquinone) iron-sulfur protein 6 (Ndufs6) is depressed in MSCs. Similar Ndufs6 knockout...
The beneficial functions of mesenchymal stem cells (MSCs) decline with age, limiting their therapeutic efficacy for myocardial infarction (MI). Macrophage migration inhibitory factor (MIF) promotes cell proliferation and survival. We investigated whether MIF overexpression could rejuvenate aged MSCs increase in MI. Young were isolated from the bone marrow young donors. MSCs, MIF-overexpressing transplanted into peri-infarct region a rat MI model. Aged exhibited lower proliferative capacity,...
Application of mesenchymal stem cell-derived exosomes (MSC-EXO) has emerged as a novel therapeutic strategy for myocardial infarction (MI). Our previous study showed that pretreatment with hemin, potent heme oxygenase-1 (HO-1) inducer, enhanced the cardioprotective effects MSCs in mouse model MI. This aimed to investigate EXO derived from hemin-pretreated (Hemin-MSC-EXO) MI and explore potential mechanisms.MSC-EXO Hemin-MSC-EXO were collected characterized. MSC-EXO intramuscularly injected...
Abstract Doxorubicin (DOX) is a chemotherapeutic agent widely used for tumor treatment. Nonetheless its clinical application heavily limited by cardiotoxicity. There accumulated evidence that transplantation of mesenchymal stem cell-derived exosomes (MSC-EXOs) can protect against Dox-induced cardiomyopathy (DIC). This study aimed to examine the cardioprotective effects EXOs isolated from human induced pluripotent MSCs (iPSC-MSCs) DIC and explore potential mechanisms. were cultural...
Abstract Immunomodulatory activity of mesenchymal stem cells (MSCs) is largely mediated by paracrine factors. Our previous studies showed that activation nuclear factor-kappa B (NF-κB) regulates cytokine/growth factor secretion MSCs. This study aimed to elucidate the role Rap1 (repressor/activator protein), a novel modulator involved in NF-κB pathway, regulating immunomodulatory potency MSCs acute allograft rejection heart transplantation. The immunosuppressive wild-type (WT-MSCs) or...
Abstract Adult mesenchymal stem cells (MSCs) are immunoprivileged due to the low expression of major histocompatibility complex (MHC) II molecules. However, MHC molecules in human-induced pluripotent (iPSCs)-derived MSCs has not been investigated. Here, we examined human leukocyte antigen (HLA) derived from iPSCs, fetuses, and adult bone marrow (BM) after stimulation with interferon-γ (IFN-γ), compared their repair efficacy, cell retention, inflammation, HLA immune humanized NOD Scid gamma...
Bone osteogenic sarcoma has a poor prognosis as the exact cell of origin and signaling pathways underling tumor formation remain undefined. Here, we report an mouse model based on conditional knockout liver kinase b1 (Lkb1; also known Stk11) in Cathepsin K (Ctsk)-Cre expressing cells. Lineage tracing studies demonstrated that Ctsk-Cre could label population periosteal The cells functioned mesenchymal progenitors with regard to markers functional properties. LKB1 deficiency increased...
Rationale: Despite recent studies indicating a crucial role of ecto-5′-nucleotidase (CD73) on T cells in cardiac injury after ischemia/reperfusion, the involvement CD73 + regulatory (Tregs) repair post-myocardial infarction (MI) remains unclear.We sought to investigate contribution Tregs resolution inflammation and remodeling MI.Methods: Cardiac function, tissue injury, percentage injured hearts, purinergic signaling changes FoxP3 were analyzed permanent descending coronary artery...
Although transplantation of adult bone marrow mesenchymal stem cells (BM-MSCs) holds promise in the treatment for pulmonary arterial hypertension (PAH), poor survival and differentiation potential BM-MSCs have limited their therapeutic efficiency. Here, we compared efficacy human embryonic cell-derived MSCs (hESC-MSCs) with PAH an animal model. One week following monocrotaline (MCT)-induced PAH, mice were randomly assigned to receive phosphate-buffered saline (MCT group); 3.0×10(6)...
Mesenchymal stem cells (MSCs) can improve cutaneous wound healing via the secretion of growth factors. However, therapeutic efficacy MSCs varies depending upon their source. Induced pluripotent are emerging as a promising source with potential to overcome several limitations adult MSCs. This study compared effectiveness conditioned medium derived from induced (iMSC-CdM) that umbilical cord (uMSC-CdM) in mouse model. We also investigated mechanisms protection.The iMSC-CdM or uMSC-CdM were...
Idiopathic pulmonary fibrosis (IPF) is an age-related disease with no cure. Mesenchymal stem cell (MSC)-based therapy has emerged as a novel strategy for IPF treatment. Nevertheless, MSCs derived from patients (IPF-MSCs) become senescent, thereby reducing their beneficial effects in IPF. MicroRNAs (miRNAs) mediate the senescence of MSCs, but underlying mechanisms are not fully understood. We investigated by which miR-199a-5p regulates IPF-MSC and whether its inhibition could rejuvenate...