A5019744464- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Biosensors and Analytical Detection
- Alzheimer's disease research and treatments
- Mitochondrial Function and Pathology
- Cancer-related gene regulation
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Tryptophan and brain disorders
- Cholinesterase and Neurodegenerative Diseases
- Extraction and Separation Processes
- Autophagy in Disease and Therapy
- Extracellular vesicles in disease
- DNA and Nucleic Acid Chemistry
- Metabolism, Diabetes, and Cancer
- RNA and protein synthesis mechanisms
- Immunotherapy and Immune Responses
- Oral and Maxillofacial Pathology
- Synthetic Organic Chemistry Methods
The University of Texas at Austin
2022-2025
University of Illinois Urbana-Champaign
2020-2025
University of Chicago
2021-2025
Shenzhen University Health Science Center
2024
Sichuan University
2023
The First Affiliated Hospital, Sun Yat-sen University
2023
Sun Yat-sen University
2023
Markey Cancer Center
2017-2022
University of Kentucky
2017-2022
Shanghai Jiao Tong University
2010-2021
Abstract Circular RNAs (circRNAs) represent a class of widespread and diverse endogenous that may regulate gene expression in eukaryotes. However, the regulation function human circRNAs remain largely unknown. Here we generate ribosomal-depleted RNA sequencing data from six normal tissues seven cancers, detect at least 27,000 circRNA candidates. Many these are differently expressed between cancerous tissues. We further characterize one abundant derived Exon2 HIPK3 gene, termed circHIPK3. The...
Extracellular vesicles (EVs) contain a rich cargo of different RNA species with specialized functions and clinical applications. However, the landscape characteristics extracellular vesicle long (exLR) in human blood remain largely unknown.We presented an optimized strategy for exLR sequencing (exLR-seq) plasma. The sample cohort included 159 healthy individuals, 150 patients cancer (5 types), 43 other diseases. Bioinformatics approaches were used to analyze distribution features exLRs....
Cancer cells possess a unique metabolic phenotype that allows them to preferentially utilize glucose through aerobic glycolysis. This phenomenon is referred as the “Warburg effect.” Accumulating evidence suggests microRNAs (miRNAs), class of small noncoding regulatory RNAs, interact with oncogenes/tumor suppressors and induce such reprograming in cancer cells. To systematically study roles miRNAs cells, we developed gain‐of‐function miRNA screen HeLa Subsequent investigation characterized...
Visualizing redox-active metal ions, such as Fe
Abstract As the “master” microRNA that is induced by hypoxia, miR-210 involved in multiple processes hypoxia pathway. However, whether mediates hypoxia-induced tumor cell metastasis still remains unclear. Here, we demonstrate frequently up-regulated hepatocellular carcinoma (HCC) samples and promotes migration invasion of HCC cells. Furthermore, can be cells metastasis. We identify vacuole membrane protein 1 (VMP1) as direct functional downstream target miR-210; addition, show its expression...
A powerful way to identify driver genes with causal roles in carcinogenesis is detect genomic regions that undergo frequent alterations cancers. Here we identified 1,241 of somatic copy number 58 paired hepatocellular carcinoma (HCC) tumors and adjacent nontumor tissues using genome-wide single nucleotide polymorphism (SNP) 6.0 arrays. Subsequently, by integrating profiles gene expression signatures derived from the same HCC patients, 362 differentially expressed within aberrant regions....
Abstract Circular RNAs (circRNAs) are an intriguing class of widely prevalent endogenous RNAs, the vast majority which have not been characterized functionally. Here, we identified a novel oncogenic circRNA originating from back‐splicing Exon2 and Exon3 tumor suppressor gene, ARHGAP35 (also known as P190‐A), termed circARHGAP35. observe that circARHGAP35 linear antithetical expression functions. Interestingly, contains 3867 nt long ORF with m 6 A‐modified start codon encodes truncated...
Abstract BRD4 assembles transcriptional machinery at gene super-enhancer regions and governs the expression of genes that are critical for cancer progression. However, it remains unclear whether BRD4-mediated transcription is required tumor cells to develop drug resistance. Our data show prolonged treatment luminal breast with AKT inhibitors induces FOXO3a dephosphorylation, nuclear translocation, disrupts its association SirT6, eventually leading acetylation as well recognition. Acetylated...
Regulating cell–cell interactions and cell behaviors via surface engineering is of significance for biological research such as fate control therapy. While extensive efforts have been made to induce assembly various modifications triggered by macromolecules or organic metabolites, controllable that include both disassembly metal ions remain a challenge. Herein, we report strategy based on DNAzymes realize interactions, ions. The metal-dependent DNAzyme-based cleavage can effectively...
Detecting metal ions in vivo with a high spatiotemporal resolution is critical to understanding the roles of both healthy and disease states. Although controls metal-ion sensors using light have been demonstrated, lack penetration depth tissue has limited their application. To overcome this limitation, we herein report use high-intensity focused ultrasound (HIFU) remotely deliver on-demand, spatiotemporally resolved thermal energy activate DNAzyme at targeted region vitro vivo. A...
DNA aptamers have been widely used as biosensors for detecting a variety of targets. Despite decades success, they not applied to monitor any targets in plants, even though plants are major platform providing oxygen, food, and sustainable products ranging from energy fuels chemicals, high-value such pharmaceuticals. A barrier progress is lack efficient methods deliver into plant cells. We herein report thiol-mediated uptake method that more efficiently delivers Arabidopsis tobacco leaf cells...
Following tissue damage, epithelial stem cells (SCs) are mobilized to enter the wound, where they confront harsh inflammatory environments that can impede their ability repair injury. Here, we investigated mechanisms protect skin SCs within this environment. Characterization of gene expression profiles hair follicle (HFSCs) migrated into wound site revealed activation an immune-modulatory program, including CD80, major histocompatibility complex class II (MHCII), and CXC motif chemokine...
// Jiwei Zhang 1, * , Bojian Fei 2, Qifeng Wang 3 Mingxu Song 1 Yuan Yin Binbin Shujuan Ni Weijie Guo 4 Zehua Bian Chao Quan Zhihui Liu Yugang 5 Jian Yu 6 Xiang Du Dong Hua Zhaohui Huang Wuxi Oncology Institute, the Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China 2 Department Surgical Oncology, Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, Medical College, Urology, Michigan Comprehensive Ann Arbor, MI 48109-5942, USA State Key Laboratory...
MicroRNAs (miRNA) that are strongly implicated in carcinogenesis have recently reshaped our understanding of the role non-protein-coding RNAs. Here, we focused on function and molecular mechanism miR-202-3p its potential clinical application colorectal cancer.miR-202-3p expression was determined by quantitative reverse transcriptase PCR (qRT-PCR) 94 cancer tissues corresponding noncancerous (NCT). Cell proliferation colony formation assays vitro xenograft experiments vivo were used to...
MicroRNAs (miRNAs) are small, single-stranded, non-coding RNAs that play pivotal roles in human cancer development and progression, such as tumor metastasis. Here, we identified the miRNAs regulate hepatocellular carcinoma (HCC) cell migration by a high-throughput screening method using classical wound-healing assay with time-lapse video microscopy validation transwell assay. Eleven (miR-134, -146b-3p, -188-3p, -525-3p, -661, -767-5p, -891a, -891b, -1244, -1247 miR-1471) were found to...
The precise functions and mechanisms of micro RNA s (miR) in gallbladder cancer ( GBC ) remain elusive. In this study, we found that miR‐135a‐5p expression is often dampened correlated with neoplasm histologic grade . Micro ‐135a‐5p introduction clearly inhibited cell proliferation vitro vivo Moreover, very low density lipoprotein receptor VLDLR ), which upregulated tissues, was identified as a direct functional target miR‐135a‐5p. Furthermore, the p38 MAPK pathway proven to be involved...
Lithium has been a drug for bipolar disorders (BD) over 70 years; however, its usage limited by narrow therapeutic window (between 0.6 and 1.2 mM). Understanding the cellular distribution of lithium ions (Li+) in patient cells will offer deep insight into this limitation, but selective imaging Li+ living under biomedically relevant concentration ranges not achieved. Herein, we report vitro selection development Li+-specific DNAzyme fluorescent sensor with >100-fold selectivity other...
Despite the promise of combination cancer therapy, it remains challenging to develop targeted strategies that are nontoxic normal cells. Here we report a therapeutic strategy based on engineered DNAzyme molecular machines can promote apoptosis via dynamic inter- and intracellular regulation. To achieve external regulation T-cell/cancer cell interactions, designed DNAzyme-based machine with an aptamer i-motif, as MUC-1-selective allows specific recognition The i-motif is folded under tumor...
Abstract Transmembrane channels play a vital role in regulating the permeation process, and have inspired recent development of biomimetic channels. Herein, we report class artificial nanochannels based on DNAzyme-functionalized glass nanopipettes to realize delicate control channel permeability, whereby surface wettability charge can be tuned by metal ions DNAzyme-substrates, allowing reversible conversion between different permeability states. We demonstrate that reversibly switched four...