A5088137198- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer Immunotherapy and Biomarkers
- RNA Research and Splicing
- Immune cells in cancer
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Ferroptosis and cancer prognosis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Extracellular vesicles in disease
- Cancer, Hypoxia, and Metabolism
- Genetic factors in colorectal cancer
- Cell Image Analysis Techniques
- Cancer Genomics and Diagnostics
- AI in cancer detection
- Cancer Research and Treatments
- Gastric Cancer Management and Outcomes
- Cancer, Lipids, and Metabolism
- Pancreatic and Hepatic Oncology Research
- Colorectal Cancer Treatments and Studies
- Monoclonal and Polyclonal Antibodies Research
- Helicobacter pylori-related gastroenterology studies
- Semiconductor Quantum Structures and Devices
Wuxi Fourth People's Hospital
2016-2025
Jiangnan University
2015-2025
Sun Yat-sen University
2023-2025
Hunan University
2025
Hunan Normal University
2025
Hunan Provincial People's Hospital
2025
Jiangxi Provincial Cancer Hospital
2024
Southern Medical University
2022-2024
Nanfang Hospital
2022-2024
First Hospital of China Medical University
2022-2024
MicroRNA (miRNA) opens up a new field for molecular diagnosis of cancer. However, the role circulating miRNAs in plasma/serum cancer is not clear. The aim this study was to investigate whether plasma can be used as biomarkers early detection colorectal carcinoma (CRC). We measured levels 12 (miR-134, -146a, -17-3p, -181d, -191, -221, -222, -223, -25, -29a, -320a and -92a) samples from patients with advanced neoplasia (carcinomas adenomas) healthy controls using real-time RT-PCR. found that...
Abstract Recent preliminary studies reported the in vitro tumor-promoting effects of long non-coding RNA urothelial carcinoma associated 1 (UCA1) colorectal cancer (CRC). However, vivo functions and molecular mechanism UCA1 CRC remain unclear. Therefore, we investigated detailed role CRC. We found that was up-regulated CRCs negatively correlated with survival time two cohorts. Functional assays revealed growth-promoting function can decrease sensitivity cells to 5-FU by attenuating...
Purpose: Long non-coding RNAs (lncRNAs) play key roles in human cancers. Here, FEZF1-AS1, a highly overexpressed lncRNA colorectal cancer, was identified by microarrays. We aimed to explore the and possible molecular mechanisms of FEZF1-AS1 cancer.Experimental Design: LncRNA expression cancer tissues measured microarray qRT-PCR. The functional were demonstrated series vitro vivo experiments. RNA pull-down, immunoprecipitation luciferase analyses used demonstrate potential FEZF1-AS1.Results:...
Purpose: Tumor-associated macrophages (TAMs) are frequently associated with poor prognosis in human cancers. However, the effects of TAMs colorectal cancer contradictory. We therefore investigated functions, mechanisms, and clinical significance cancer.Experimental Design: measured macrophage infiltration (CD68), P-gp, Bcl2 expression tissues using IHC staining. Coculture cells both vitro vivo models was used to evaluate on chemoresistance. Cytokine antibody arrays, ELISA, neutralizing...
Purpose: The long, noncoding RNA (lncRNA) PVT1 is an important epigenetic regulator with a critical role in human tumors. Here, we aimed to investigate the clinical application and potential molecular mechanisms of gastric cancer tumorigenesis progression.Experimental Design: expression level was determined by RT-qPCR analysis 190 pairs tissues adjacent normal mucosa (ANT). biologic functions were assessed vitro vivo functional experiments. protein pull-down assays LS/MS mass spectrometry...
Colorectal cancer (CRC) is a major cause of death worldwide. Sensitive, non-invasive diagnostic screen methods are urgently needed to improve its survival rates. Stable circulating microRNA offers unique opportunities for the early diagnosis several diseases, including cancers. Our aim has been find new plasma miRNAs that can be used as biomarkers detection CRC.According results miRNA profiling performed on pooling samples form 10 CRC patients or healthy controls, panel (hsa-miR-10a, -19a,...
miR-204-5p was found to be downregulated in colorectal cancer tissues our preliminary microarray analyses. However, the function of remains unknown. We therefore investigated role, mechanism, and clinical significance development progression.We measured expression determined its correlation with patient prognoses. Ectopic cells, xenografts, pulmonary metastasis models used evaluate effects on proliferation, migration, chemotherapy sensitivity. Luciferase assay Western blotting were performed...
Extracellular vesicles (EVs) are small membranous that contain an abundant cargo of different RNA species with specialized functions and clinical implications. Here, we introduce updated online database (http://www.exoRBase.org), exoRBase 2.0, which is a repository EV long RNAs (termed exLRs) derived from RNA-seq data analyses diverse human body fluids. In the number exLRs has increased to 19 643 messenger (mRNAs), 15 645 non-coding (lncRNAs) 79 084 circular (circRNAs) obtained ∼1000 blood,...
Tumor-associated macrophages (TAMs) are one of the most abundant cell types in colorectal cancer (CRC) tumor microenvironment (TME). Recent studies observed complicated "cross-talks" between cells and TME. However, underlying mechanisms still poorly elucidated. Here, PD-L1 levels very low CRC but highly TAMs, a specific PD-L1+ CD206+ macrophage subpopulation identified, which is induced by associated with poor prognosis. Mechanistic investigations reveal that can secrete small extracellular...
MicroRNAs (miRNAs) are strongly implicated in cancer but their specific roles and functions the major cancers have yet to be fully elucidated. In this study, we defined oncogenic significance function of miR-95, which found elevated colorectal (CRC) tissues by microarray analysis. Evaluation an expanded CRC cohort revealed that miR-95 expression was up-regulated nearly half tumors examined (42/87) compared with corresponding noncancerous tissues. Ectopic overexpression human cell lines...
Evaluating expression of the human epidermal growth factor receptor 2 (HER2) by visual examination immunohistochemistry (IHC) on invasive breast cancer (BCa) is a key part diagnostic assessment BCa due to its recognized importance as predictive and prognostic marker in clinical practice. However, scoring HER2 subjective, consequently prone interobserver variability. Given therapeutic implications scoring, more objective method required. In this paper, we report recent automated contest, held...
Long intergenic non-coding RNA 152 (LINC00152) is a recently identified tumor-promoting long RNA. However, the biological functions of LINC00152 in colorectal cancer (CRC) remain unclear and require further research. The aim present study to explore roles cellular function its possible molecular mechanism. In this study, we discovered that was overexpressed CRC tissues negatively related survival time patients. Functional analyses revealed could promote cell proliferation. Furthermore,...
Nano-sized extracellular vesicles secreted by cells play key roles in intercellular crosstalk, and appear to be an excellent biocompatible material as therapeutic cargoes vivo. Previously, we have demonstrated that miR-204-5p is a tumor suppressor could inhibit growth, metastasis chemoresistance.A HEK293T cell line stably expressing (293T-miR-204) was constructed lentivirus transduction. Fluorescence real-time quantitative PCR (qPCR) applied measure the expression of miR-204-5p. CCK-8 colony...
Abstract Background Colorectal cancer (CRC) is a major cause of cancer-related deaths worldwide, and chemoresistance obstacle in its treatment. Despite advances therapy, the molecular mechanism underlying CRC not fully understood. Recent studies have implicated key roles long noncoding RNAs (lncRNAs) regulation chemoresistance. Methods In this study, we investigated role lncRNA LINC01852 expression was evaluated multiple cohorts using quantitative reverse transcription PCR. We conducted...
Abstract Radioresistance is a major challenge in tumor radiotherapy and involves mixture of cellular events, including ferroptosis, new type programmed cell death characterized by the excess accumulation iron-dependent lipid peroxides. In present study, we observed that surviving cancer tissues cells after had significantly greater glutathione to oxidized (GSH/GSSG) ratios lower reactive oxygen species (ROS) malondialdehyde (MDA) levels than nonirradiated tumors cells. Untargeted lipidomic...