A5083302135- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- RNA Research and Splicing
- Helicobacter pylori-related gastroenterology studies
- Gastric Cancer Management and Outcomes
- Cancer-related gene regulation
- Gut microbiota and health
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Cell Adhesion Molecules Research
- Metastasis and carcinoma case studies
- Cancer, Lipids, and Metabolism
- IL-33, ST2, and ILC Pathways
- Gastrointestinal Tumor Research and Treatment
- Colorectal Cancer Screening and Detection
- Gastroesophageal reflux and treatments
- Cancer, Hypoxia, and Metabolism
- Cancer Genomics and Diagnostics
- Mechanisms of cancer metastasis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Colorectal Cancer Surgical Treatments
Jiangnan University
2016-2025
Wuxi Fourth People's Hospital
2016-2025
Nanjing Drum Tower Hospital
2024
Cancer Institute (WIA)
2022
Soochow University
2012-2014
Second Affiliated Hospital of Soochow University
2013-2014
Wuxi No.2 People's Hospital
2013
Shanghai Cancer Institute
2011
Abstract Recent preliminary studies reported the in vitro tumor-promoting effects of long non-coding RNA urothelial carcinoma associated 1 (UCA1) colorectal cancer (CRC). However, vivo functions and molecular mechanism UCA1 CRC remain unclear. Therefore, we investigated detailed role CRC. We found that was up-regulated CRCs negatively correlated with survival time two cohorts. Functional assays revealed growth-promoting function can decrease sensitivity cells to 5-FU by attenuating...
Purpose: Long non-coding RNAs (lncRNAs) play key roles in human cancers. Here, FEZF1-AS1, a highly overexpressed lncRNA colorectal cancer, was identified by microarrays. We aimed to explore the and possible molecular mechanisms of FEZF1-AS1 cancer.Experimental Design: LncRNA expression cancer tissues measured microarray qRT-PCR. The functional were demonstrated series vitro vivo experiments. RNA pull-down, immunoprecipitation luciferase analyses used demonstrate potential FEZF1-AS1.Results:...
Purpose: Tumor-associated macrophages (TAMs) are frequently associated with poor prognosis in human cancers. However, the effects of TAMs colorectal cancer contradictory. We therefore investigated functions, mechanisms, and clinical significance cancer.Experimental Design: measured macrophage infiltration (CD68), P-gp, Bcl2 expression tissues using IHC staining. Coculture cells both vitro vivo models was used to evaluate on chemoresistance. Cytokine antibody arrays, ELISA, neutralizing...
miR-204-5p was found to be downregulated in colorectal cancer tissues our preliminary microarray analyses. However, the function of remains unknown. We therefore investigated role, mechanism, and clinical significance development progression.We measured expression determined its correlation with patient prognoses. Ectopic cells, xenografts, pulmonary metastasis models used evaluate effects on proliferation, migration, chemotherapy sensitivity. Luciferase assay Western blotting were performed...
Tumor-associated macrophages (TAMs) are one of the most abundant cell types in colorectal cancer (CRC) tumor microenvironment (TME). Recent studies observed complicated "cross-talks" between cells and TME. However, underlying mechanisms still poorly elucidated. Here, PD-L1 levels very low CRC but highly TAMs, a specific PD-L1+ CD206+ macrophage subpopulation identified, which is induced by associated with poor prognosis. Mechanistic investigations reveal that can secrete small extracellular...
Long intergenic non-coding RNA 152 (LINC00152) is a recently identified tumor-promoting long RNA. However, the biological functions of LINC00152 in colorectal cancer (CRC) remain unclear and require further research. The aim present study to explore roles cellular function its possible molecular mechanism. In this study, we discovered that was overexpressed CRC tissues negatively related survival time patients. Functional analyses revealed could promote cell proliferation. Furthermore,...
Abstract Background Colorectal cancer (CRC) is a major cause of cancer-related deaths worldwide, and chemoresistance obstacle in its treatment. Despite advances therapy, the molecular mechanism underlying CRC not fully understood. Recent studies have implicated key roles long noncoding RNAs (lncRNAs) regulation chemoresistance. Methods In this study, we investigated role lncRNA LINC01852 expression was evaluated multiple cohorts using quantitative reverse transcription PCR. We conducted...
ABSTRACT MicroRNAs (miRNAs) that exert function by posttranscriptional suppression have recently brought insight in our understanding of the role non-protein-coding RNAs carcinogenesis and metastasis. In this study, we described molecular mechanism miR-139-5p colorectal cancer (CRC) its potential clinical application CRC. We found was significantly downregulated 73.8% CRC samples compared with adjacent noncancerous tissues (NCTs), decreased associated poor prognosis. Functional analyses...
Gastric cancer (GC) is one of the most common cancers and leading cause cancer-related deaths globally. The discovery microRNAs (miRNAs) provides a new avenue for GC diagnostic treatment regiments. Currently, large number miRNAs have been reported to be associated with progression GC, among which miR-378 has examined downregulated in tissues several cell lines. However, function on cells mechanisms were less known. Here we found that ectopic expression could inhibit proliferation, cycle...
// Jiwei Zhang 1, * , Bojian Fei 2, Qifeng Wang 3 Mingxu Song 1 Yuan Yin Binbin Shujuan Ni Weijie Guo 4 Zehua Bian Chao Quan Zhihui Liu Yugang 5 Jian Yu 6 Xiang Du Dong Hua Zhaohui Huang Wuxi Oncology Institute, the Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214062, China 2 Department Surgical Oncology, Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, Medical College, Urology, Michigan Comprehensive Ann Arbor, MI 48109-5942, USA State Key Laboratory...
Small nucleolar RNA host gene (SNHG) long noncoding RNAs (lncRNAs) are frequently dysregulated in human cancers and involved tumorigenesis progression. SNHG17 has been reported as a candidate oncogene several cancer types, however, its regulatory role colorectal (CRC) is unclear.SNHG17 expression multiple CRC cohorts was assessed by RT-qPCR or bioinformatic analyses. Cell viability evaluated using Counting Kit-8 (CCK-8) colony formation assays. mobility invasiveness were Transwell Tumor...
Abstract Background Long noncoding RNAs (lncRNAs) have driven research focused on their effects as oncogenes or tumor suppressors involved in carcinogenesis. However, the functions and mechanisms of most lncRNAs colorectal cancer (CRC) remain unclear. Methods The expression DLGAP1-AS2 was assessed by quantitative RT-PCR multiple CRC cohorts. impacts growth metastasis were evaluated a series vitro vivo assays. Furthermore, underlying mechanism revealed RNA pull down, immunoprecipitation,...
Abstract Background SLCO4A1-AS1 was found to be upregulated in several cancer types, including colorectal (CRC). However, the detailed roles of CRC remain elucidated. Therefore, we investigated functions, mechanism, and clinical significance tumourigenesis. Methods We measured expression tissues using qRT-PCR determined its correlation with patient prognosis. Promoter methylation analyses were used assess status SLCO4A1-AS1. Gain- loss-of-function assays evaluate effects on growth vitro...
Abstract The incidence and death rate of colorectal cancer ( CRC ) is very high, which brings great need to understand the early molecular events . These studies demonstrate that long noncoding RNA (lnc plays an important role in occurrence development human cancer. Small nucleolar host gene 15 SNHG 15) was recently identified as a cancer‐related lnc In this study, we aimed evaluate function mechanism expression detected by quantitative RT ‐ PCR qRT tissues matched noncancerous NCT s). CCK...
Background: Association studies have linked microbiome alterations with colorectal cancer (CRC). However, differences in tumor, para-cancerous, normal mucosal, and fecal microbiota remain to be strengthened. Methods: We performed a study on the ecologically rich taxonomically diverse of gut using three types mucosa (tumor mucosa, para-cancerous mucosa) feces from 98 CRC patients. Additionally, we profiled occult blood test (FOBT) positive negative groups at different sampling sites. Results:...
Abstract Background Accumulating evidences have shown that long noncoding RNAs (lncRNAs) play key roles in many diseases, including cancer. Several studies reported MCM3AP antisense RNA 1 (MCM3AP‐AS1) was associated with the tumorigenesis and progression. However, specific function mechanism of MCM3AP‐AS1 colorectal cancer (CRC) not been fully understood. Methods The expression detected by quantitative reverse transcription PCR (RT‐qPCR) CRC tissues matched noncancerous (NCTs). CCK‐8 assay,...
Colorectal cancer (CRC) is one of the most common cancers worldwide, whose morbidity and mortality gradually increased. Here, we aimed to identify access prognostic long non-coding RNAs (lncRNAs) associated with overall survival (OS) in CRC. Firstly, RNA expression profiles were obtained from The Cancer Genome Atlas (TCGA) database, 439 CRC patients enrolled as a training set. Univariate Cox analysis least absolute shrinkage selection operator (LASSO) performed lncRNAs. Multivariable...
TP53 mutations are linked to aggressive progression and chemoresistance in gastric cancer (GC). Frameshift mutation is the second most common type of TP53. However, consequences this GC were not well understood, targeted therapies for patients harboring frameshift also established. Histone methylation significantly influences tumorigenesis TP53-mutated cancers, related inhibitors emerging as specific therapeutic strategies. By treating cell lines various types with a library histone...
Gastric cancer is the third leading cause of cancer-related mortality worldwide. Recent evidence points to importance cross talk between cells and surrounding stroma on gastric progression. Tumor microenvironment biomarkers thus represent a new opportunity for diagnostics innovation. Reactive stromal fibroblasts selectively express fibroblast activation protein alpha (FAP-α), homodimeric integral membrane gelatinase that belongs serine protease family. We report here FAP-α expression...
Gastric cancer patients harboring a TP53 mutation exhibit more aggressive and chemoresistant phenotype. Unfortunately, efforts to identify the vulnerabilities overcome these malignancies have made minimal progress in recent years. Therefore, there is an urgent need explore novel therapeutic strategies for this subclass. Histone methylation modulators are critical epigenetic targets therapies that help maintain of cancers mutations senescence evasion. Triggering now considered benefit...