A5100411606- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Tuberculosis Research and Epidemiology
- Biosensors and Analytical Detection
- DNA and Nucleic Acid Chemistry
- Plasmonic and Surface Plasmon Research
- Advanced Biosensing Techniques and Applications
- Mycobacterium research and diagnosis
- Optical Coatings and Gratings
- COVID-19 epidemiological studies
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced oxidation water treatment
- Extracellular vesicles in disease
- Viral Infections and Vectors
- Electrochemical Analysis and Applications
- Enzyme Catalysis and Immobilization
- Photonic Crystals and Applications
- Photonic and Optical Devices
- Fuel Cells and Related Materials
- Natural product bioactivities and synthesis
- SARS-CoV-2 detection and testing
- Viral Infections and Outbreaks Research
- Pharmaceutical and Antibiotic Environmental Impacts
Henan Provincial Chest Hospital
2025
Zhengzhou University
2025
Tianjin University
2025
Tianjin Medical University
2025
University of Illinois Urbana-Champaign
2021-2024
Bengbu Medical College
2023-2024
Urbana University
2022-2024
Southern Medical University
2023
Nanfang Hospital
2023
Shanghai University of Medicine and Health Sciences
2021-2023
Abstract Background Patients with critical illness due to infection the 2019 coronavirus disease (COVID-19) show rapid progression acute respiratory failure. The study aimed screen most useful predictive factor for caused by COVID-19. Methods prospectively involved 61 patients COVID-19 as a derivation cohort, and 54 validation cohort. was selected using LASSO regression analysis. A nomogram based on non-specific laboratory indicators built predict probability of illness. Results...
Abstract Background Severe ill patients with 2019 novel coronavirus (2019-nCoV) infection progressed rapidly to acute respiratory failure. We aimed select the most useful prognostic factor for severe illness incidence. Methods The study prospectively included 61 2019-nCoV treated at Beijing Ditan Hospital from January 13, 2020 31, 2020. Prognostic of was selected by LASSO COX regression analyses, predict probability 2019-CoV pneumonia. predictive accuracy evaluated concordance index,...
While nanoscale quantum emitters are effective tags for measuring biomolecular interactions, their utilities applications that demand single-unit observations limited by the requirements large numerical aperture (NA) objectives, fluorescence intermittency, and poor photon collection efficiency resulted from omnidirectional emission. Here, we report a nearly 3000-fold signal enhancement achieved through multiplicative effects of enhanced excitation, highly directional extraction, improvement,...
The standard shotgun proteomics data analysis strategy relies on searching MS/MS spectra against a context-independent protein sequence database derived from the complete genome of an organism. Because transcriptome (RNA-Seq) promises unbiased and comprehensive picture transcriptome, we reason that sample-specific RNA-Seq can better approximate real pool in sample thus improve identification. In this study, have developed two-step for building databases data. First, size is reduced by...
In recent years, a lot of efforts have been made in conformational epitope prediction as antigen proteins usually bind antibodies with an assembly sequentially discontinuous and structurally compact surface residues. Currently, only few methods for spatial are available focus on single residue propensity scales or continual segments clustering. the method SEPPA, concept ‘unit patch triangle’ was introduced to better describe local context protein surface. Besides that, SEPPA incorporated...
Abstract Systematically controlling the morphology of nanoparticles, especially those growing from gold nanorod (AuNR) seeds, are underexplored; however, AuNR and its related morphologies have shown promises in many applications. Herein we report use programmable DNA sequences to control overgrowth, resulting nanoparticles varying nanodumbbell nanooctahedron, as well shapes between, with high yield reproducibility. Kinetic studies revealed two representative pathways for shape evolving into...
Abstract Developing a comprehensive platform which has both diagnosis and therapeutic strategies is necessary for efficient tumor treatment. In this work, F uel I mproved micro R NA E xplorer (FIRE) probe with signal amplification capability designed sensitive detection of microRNA‐21 (miR‐21), upregulated in most cells. Besides, FIRE could be loaded by polyethylenimine (PEI)‐modified gold nanorods (AuNR‐PEI) via facile electrostatic interaction, avoid the complicated processes commonly used...
Tocilizumab has been reported to attenuate the "cytokine storm" in COVID-19 patients. We attempted verify effectiveness and safety of tocilizumab therapy identify patients most likely benefit from this treatment. conducted a randomized, controlled, open-label multicenter trial among The were randomly assigned 1:1 ratio receive either addition standard care or alone. cure rate, changes oxygen saturation interference, inflammation biomarkers observed. Thirty-three randomized group, 32 control...
Detecting metal ions in vivo with a high spatiotemporal resolution is critical to understanding the roles of both healthy and disease states. Although controls metal-ion sensors using light have been demonstrated, lack penetration depth tissue has limited their application. To overcome this limitation, we herein report use high-intensity focused ultrasound (HIFU) remotely deliver on-demand, spatiotemporally resolved thermal energy activate DNAzyme at targeted region vitro vivo. A...
Exosomal microRNAs (miRNAs) have considerable potential as pivotal biomarkers to monitor cancer development, dis-ease progression, treatment effects and prognosis. Here, we report an efficient target recycling amplification process (TRAP) for the digital detection of miRNAs using photonic resonator absorption microscopy. We achieve multiplex with sub-attomolar sensitivity in 20 minutes, robust selectivity single nucleotide variants, a broad dynamic range from 1 aM pM. Compared traditional...
Coronavirus transmission and mutations have brought intensive challenges on pandemic control disease treatment. Developing robust versatile antiviral drugs for viral neutralization is highly desired. Here, we created a new polyvalent nanobody (Nb) structure that shows the effective inhibition of SARS-CoV-2 infections. Our Nb structure, called "PNS", achieved by first conjugating single-stranded DNA (ssDNA) receptor-binding domain (RBD)-targeting with retained binding ability to spike protein...
Rapid, ultrasensitive, and selective quantification of circulating microRNA (miRNA) biomarkers in body fluids is increasingly deployed early cancer diagnosis, prognosis, therapy monitoring. While nanoparticle tags enable detection nucleic acid or protein with digital resolution subfemtomolar limits without enzymatic amplification, the response time these assays typically dominated by diffusion-limited transport analytes nanotags to biosensor surface. Here, we present a magnetic activate...
Several applications in health diagnostics, food, safety, and environmental monitoring require rapid, simple, selective, quantitatively accurate viral load monitoring. Here, we introduce the first label-free biosensing method that rapidly detects quantifies intact virus human saliva with single-virion resolution. Using pseudotype SARS-CoV-2 as a representative target, immobilize aptamers ability to differentiate active from inactive virions on photonic crystal, where are captured through...
Herein, we report the development of a new "smart" radioactive probe (i.e., 1) which can undergo furin-controlled condensation and self-assembly nanoparticles 1-NPs) in tumor cells its application for enhanced microPET imaging tumors nude mice co-injected with cold analog 1-Cold). Furin-controlled 1-Cold 1-Cold-NPs) vitro were validated characterized HPLC, mass spectra, SEM, TEM analyses. Cell uptake studies showed that both 1 have good cell permeability. images 1-Cold-treated MDA-MB-468...
Adverse early-life exposures program an increased risk of chronic metabolic diseases in adulthood. However, the effects genistein consumption early life on health are unclear. Our objective was to investigate whether perinatal intake could mitigate deleterious a high-fat diet (HF) metabolism dams and female offspring explore role gut microbiota mediating transgenerational effects. C57BL/6 mice were fed HF, HF with (0.6 g kg-1 diet) or normal control for 3 weeks before mating throughout...
Abstract In recent years, the experiments have revealed that plants raise cytotoxicity and anticancer potentials of iron nanoparticles against several cell lines especially tumor lines. present study, we formulated a modern drug by (FeNPs) containing Glycyrrhiza glabra L leaf for treatment acute lymphoblastic leukemia. Characterization FeNPs was done TEM, FE‐SEM, UV–Vis, FT‐IR. FE‐SEM TEM images an average diameter 11–18 nm nanoparticles. UV–Vis absorption band at 298 is related to surface...
Abstract Small extracellular–vesicule-associated microRNA (sEV-miRNA) is an important biomarker for cancer diagnosis. However, rapid and sensitive detection of low-abundance sEV-miRNA in clinical samples challenging. Herein, a simple electrochemical biosensor that uses DNA nanowire to localize catalytic hairpin assembly (CHA), also called domino-type localized (DT-LCHA), has been proposed sEV-miRNA1246 detection. The DT-LCHA offers triple amplification, (i). CHA system was nanowire, which...