A5080012492- MicroRNA in disease regulation
- Bone Metabolism and Diseases
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Microtubule and mitosis dynamics
- Cardiomyopathy and Myosin Studies
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Advanced biosensing and bioanalysis techniques
- Cellular Mechanics and Interactions
- Mesenchymal stem cell research
- Inflammasome and immune disorders
- Sperm and Testicular Function
- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Polyamine Metabolism and Applications
- Spine and Intervertebral Disc Pathology
- Meat and Animal Product Quality
- Animal Nutrition and Physiology
- Musculoskeletal pain and rehabilitation
- Adenosine and Purinergic Signaling
- Lipid Membrane Structure and Behavior
- Bone health and treatments
- RNA Interference and Gene Delivery
Shandong Province Animal Husbandry and Veterinary Bureau
2015-2025
Fudan University
2001-2025
Minhang District Central Hospital
2017-2024
China Southern Power Grid (China)
2024
Yale University
2011-2022
Whitney Museum of American Art
2022
Northeast Normal University
2022
Chinese Academy of Sciences
2011-2020
Kunming Institute of Zoology
2013-2020
Weatherford College
2020
Due to oxidative damage and mitochondrial dysfunction, boar semen cryopreservation remains a significant challenge. This study investigates the effects of pyrroloquinoline quinone (PQQ), mitochondrial-targeted antioxidant, on post-thaw sperm quality during cryopreservation. Boar was diluted in freezing extender containing different concentrations PQQ (0, 10, 100, 1000, 10,000 nM). After freezing-thawing, motility, viability, acrosome integrity, activity, adenosine triphosphate (ATP) levels,...
Microtubule-based transport by the kinesin motors, powered ATP hydrolysis, is essential for a wide range of vital processes in eukaryotes. We obtained insight into this process developing atomic models no-nucleotide and states monomeric motor domain on microtubules from cryo-EM reconstructions at 5-6 Å resolution. By comparing these with existing X-ray structures ADP-bound kinesin, we infer mechanistic scheme which microtubule attachment, mediated universally conserved 'linchpin' residue...
Septin 9 (SEPT9) interacts with microtubules (MTs) and is mutated in hereditary neuralgic amyotrophy (HNA), an autosomal-dominant neuropathy. The mechanism of SEPT9 interaction MTs the molecular basis HNA are unknown. Here, we show that N-terminal domain contains novel repeat motifs K/R-x-x-E/D R/K-R-x-E, which bind bundle by interacting acidic C-terminal tails β-tubulin. Alanine scanning mutagenesis revealed K/R-R/x-x-E/D pair electrostatically one another β-tubulin, enabling septin–septin...
Myosin X has features not found in other myosins. Its structure must underlie its unique ability to generate filopodia, which are essential for neuritogenesis, wound healing, cancer metastasis and some pathogenic infections. By determining high-resolution structures of key components this motor, characterizing the vitro behaviour native dimer, we identify that explain myosin dimer behaviour. Single-molecule studies demonstrate a moves on actin bundles with higher velocities takes larger...
The DNA-origami technique has enabled the engineering of transmembrane nanopores with programmable size and functionality, showing promise in building biosensors synthetic cells. However, it remains challenging to build large (>10 nm), functionalizable that spontaneously perforate lipid membranes. Here, we take advantage pneumolysin (PLY), a bacterial toxin potently forms wide ring-like channels on cell membranes, construct hybrid DNA-protein nanopores. This PLY-DNA-origami complex, which...
Ionizing radiation is known to cause DNA damage, including single-strand and double-strand breaks (DSBs), the unrepair of particularly DSBs, may chromosome aberrations. Although etiology gliomas remains unclear, exposure ionizing has been identified as only established risk factor. We hypothesized that polymorphisms candidate genes involved in DSBs repair pathway contribute susceptibility glioma. used a haplotype-based approach investigate role 22 tagging single-nucleotide (tSNPs) XRCC5 ,...
Significance Cofilin is an essential actin regulatory protein that severs filaments, which accelerates network remodeling by increasing the concentration of filament ends available for elongation and subunit exchange. The molecular basis how cofilin binding interactions fragment have stiffness comparable to commercial laboratory plastics, remains a central unresolved mystery cellular cytoskeleton reorganization. In this study we demonstrate severing vertebrate driven linked dissociation...
Abstract Sperm are susceptible to excessive reactive oxygen species (ROS). Spermine and spermidine secreted in large amounts by the prostate potent natural free radical scavengers protect cells against redox disorder. Thus, we used boar sperm as a model study polyamines uptake elucidate whether protected from ROS stress. Seven mature fertile Duroc boars (aged 15 30 mo) were this study. In experiment 1, spermine (3.6 ± 0.3 3.3 0.2 mmol/L, respectively) abundant seminal plasma, content of...
ABSTRACT One main cause of persistent back discomfort is intervertebral disc degeneration (IDD), with inflammation and extracellular matrix (ECM) degradation playing critical roles. This study investigates the role ZIP8 , a zinc transporter, in IDD pathogenesis, focusing on its effects inflammatory responses, ECM Wnt/β‐catenin signalling pathway. was identified as hub gene from GSE27494 dataset through bioinformatics analysis. The investigated nucleus pulposus (NP) cells RAW 264.7...
Significance Pore-forming toxins are a common type of bacterial and important virulence factors. Aerolysin is produced by Aeromonas species. It interesting that aerolysin-like proteins also found in vertebrates. However, the physiological roles these still unknown. Previously, βγ-crystallin fused protein (α-subunit) trefoil factor (β-subunit) complex, hence named βγ-CAT, was identified frogs. Here, we this complex inducible challenge. The able to protect host from microbial infection....
It has been established that Adenosine-5'-triphosphate (ATP) can activate the NLRP3 inflammasome. However, physiological effect of extracellular ATP on inflammasome activation not yet investigated. In present study, we found was indeed released during bacterial infection. By using a murine peritonitis model, also promotes fight against infection in mice. induced secretion IL-1β and chemokines by bone marrow-derived macrophages vitro. Furthermore, intraperitoneal injection elevated levels...
Abstract Circular RNAs (circRNAs) serve as competing endogenous (ceRNAs) and indirectly regulate gene expression through shared microRNAs (miRNAs). However, the potential circRNAs functioning ceRNAs in osteoporosis remain unclear. The bone marrow mesenchymal stem cells (BMSCs) were isolated from ovariectomy ( OVX) mice controls. We systematically analyzed RNA‐seq miRNA‐microarray data, miRNA‐target interactions, prominently coexpressed pairs to identify aberrantly expressed circRNAs, miRNAs,...
Osteosarcoma (OS) is the most common bone tumor in children and adolescents characterized by high metastatic recurrence rates.In past, it has been shown that microRNAs may play critical roles hypoxia-related OS proliferation invasion.However, mechanisms which cells acquire this malignant phenotype have remained largely unknown.In present study, we report let-7f-5p TARBP2 were expressed lower amounts human cell lines when compared with hFOB normal osteoblastic line; however, both types of...
Osteosarcoma (OS) is the most common bone cancer worldwide. There evidence that microRNA-409 (miR-409-3p) involved in tumorigenesis and progression, however, its possible role OS requires clarification. In present study, we evaluated expression level, clinical significance, mode of action miR-409-3p OS. The levels were diminished cells tissues compared with associated adjacent non-tumor a non-cancer osteoplastic cell line. Low stage distant metastasis patients Resumption attenuated...
As a subclass of noncoding RNAs, circular RNAs (circRNAs) have been demonstrated to play critical role in regulating gene expression eukaryotes. Recent studies revealed the pivotal functions circRNAs cancer progression. Nevertheless, how participate osteosarcoma (OS) development and progression are not well understood. In present study, we identified circRNA circFAT1(e2) with an upregulated level OS tissues. By functional experiments, found that depletion significantly suppressed...
Carboxylated ε-poly-l-lysine (CPLL), a novel cryoprotectant, can protect the sperm membranes by inhibiting ice crystal formation during cryopreservation process. The present study was conducted to investigate consequence of CPLL supplementation on post-thaw quality cryopreserved goat sperm. For this, different doses (0, 0.5%, 1%, 1.5%, and 2%; v/v) were added medium, motility, membrane acrosome integrity, mitochondrial potential (MMP), ATP level, ROS production, anti-oxidant defense system,...
Osteoporosis (OP) is a disease characterized by bone mass loss, microstructure damage, increased fragility, and easy fracture. The molecular mechanism underlying OP remains unclear.In this study, we identified 217 genes associated with OP, formed gene set [OP-related (OPgset)].The highly enriched GOs pathways showed OPgset were significantly involved in multiple biological processes (skeletal system development, ossification, osteoblast differentiation), several OP-related (Wnt signaling...
Endolysosomes are key players in cell physiology, including molecular exchange, immunity, and environmental adaptation. They the targets of some pore-forming aerolysin-like proteins (ALPs) that widely distributed animals plants functionally related to bacterial toxin aerolysins. βγ-CAT is a complex an ALP (BmALP1) trefoil factor (BmTFF3) firebelly toad (Bombina maxima). It first example secreted endogenous protein modulates biochemical properties endolysosomes by inducing pore formation...