A5066745952- Antimicrobial Peptides and Activities
- Lipid Membrane Structure and Behavior
- Inflammasome and immune disorders
- Immune Response and Inflammation
- Aquaculture disease management and microbiota
- Endoplasmic Reticulum Stress and Disease
- Wound Healing and Treatments
- Adenosine and Purinergic Signaling
- Lipid metabolism and disorders
- Protease and Inhibitor Mechanisms
- Thermal Regulation in Medicine
- Nerve injury and regeneration
- Kawasaki Disease and Coronary Complications
- Herpesvirus Infections and Treatments
- Platelet Disorders and Treatments
- RNA regulation and disease
- Autophagy in Disease and Therapy
- Toxoplasma gondii Research Studies
- Immune Cell Function and Interaction
- Extracellular vesicles in disease
- IL-33, ST2, and ILC Pathways
- Cytomegalovirus and herpesvirus research
- Toxin Mechanisms and Immunotoxins
- Blood Coagulation and Thrombosis Mechanisms
- Biochemical and Structural Characterization
Kunming Medical University
2020-2021
Kunming Institute of Zoology
2012-2019
Chinese Academy of Sciences
2012-2019
Model Animal Research Center
2018
Yunnan Observatories
2013
Background: Nonscar wound healing is a desirable treatment for cutaneous wounds worldwide. Peptide OH-CATH30 (OH30) from king cobra can selectively regulate the innate immunity and create an anti-inflammatory micro-environment which might benefit nonscar healing. Purpose: To overcome enzymatic digestion control release of OH30, OH30 encapsulated in carboxymethyl chitosan nanoparticles (CMCS-OH30 NP) were prepared their effects on evaluated. Methods: CMCS-OH30 NP by mild ionic gelation method...
Antimicrobial peptides (AMPs) have been considered alternatives to conventional antibiotics for drug-resistant bacterial infections. However, their comparatively high toxicity toward eukaryotic cells and poor efficacy in vivo hamper clinical application. OH-CATH30, a novel cathelicidin peptide deduced from the king cobra, possesses potent antibacterial activity vitro. The objective of this study is evaluate OH-CATH30 its analog OH-CM6 against bacteria vitro vivo. MICs ranged 1.56 12.5 μg/ml...
Sepsis, which is a systemic inflammatory response that follows bacterial infection, has high mortality rate and limited therapeutic options. Here we show the antimicrobial peptide OH-CATH30, naturally occurs in snake, selectively regulates innate immune to protect mice from lethal sepsis. The administration of OH-CATH30 significantly improves survival infected by antibiotic-susceptible -resistant pathogens, including Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus....
It has been established that Adenosine-5'-triphosphate (ATP) can activate the NLRP3 inflammasome. However, physiological effect of extracellular ATP on inflammasome activation not yet investigated. In present study, we found was indeed released during bacterial infection. By using a murine peritonitis model, also promotes fight against infection in mice. induced secretion IL-1β and chemokines by bone marrow-derived macrophages vitro. Furthermore, intraperitoneal injection elevated levels...
ABSTRACT The therapeutic potential of antimicrobial peptides (AMPs) has been evaluated in many infectious diseases. However, the topical application AMPs for ocular bacterial infection not well investigated. AMP OH-CATH30, which was identified king cobra, exhibits potent activity. In this study, we investigated OH-CATH30 Pseudomonas aeruginosa keratitis. Ten isolates P. from individuals with keratitis were susceptible to but cefoperazone, ciprofloxacin, gentamicin, and levofloxacin....
ABSTRACT Tissue repair is a highly dynamic process, and the immediate onset of acute inflammation has been considered necessary for repair. Pore‐forming proteins are important, both in pathogen invasion host immunity. However, their roles wound healing tissue unclear. βγ‐crystallin fused aerolysin‐like protein (α‐subunit) trefoil factor (β‐subunit) complex (βγ‐CAT) bacterial pore‐forming toxin identified frog Bombina maxima . In this study, we established mouse cutaneous models to explore...
Abstract Bacterial pore-forming toxin aerolysin-like proteins (ALPs) are widely distributed in animals and plants. However, functional studies on these ALPs remain their infancy. βγ-CAT is the first example of a secreted protein that functions to modulate endolysosome pathway via endocytosis pore formation endolysosomes. specific cell surface molecules mediating action elusive. Here, actions were largely attenuated by either addition or elimination acidic glycosphingolipids (AGSLs). Further...
Many intracellular pathogens invade cells via endocytic organelles and have adapted to the drop in pH along pathway. However, strategy by which host cell counteracts this pathogen adaptation remains unclear. βγ-CAT is an aerolysin-like pore-forming protein trefoil factor complex frog Bombina maxima. We report here that βγ-CAT, as a host-secreted with intrinsic channel-forming property, first example of molecule actively neutralizes acidification counteract Listeria monocytogenes infection....
Animal models are essential for the development of new anti-infectious drugs. Although some bacterial infection have been established in rodents, small primate rare. Here, we report on two tree shrew (Tupaia belangeri chinensis). A burnt skin model was induced by dropping 5×10<sup>6</sup> CFU Staphylococcus aureus surface a wound after third degree burn. This dose S. caused persistent 7 days and obvious inflammatory response observed 4 inoculation. Dacron graft model,...
Bacterial pore-forming toxin aerolysin-like proteins are widely distributed in animals and plants. Emerging evidence supports their roles host innate immunity, but direct actions adaptive immunity remain elusive. In this study, we found that βγ-CAT, an protein trefoil factor complex identified the frog Bombina maxima, modulated several steps of endocytic pathways during dendritic cell antigen presentation. The augmented uptake cells actively neutralized acidification cellular organelles to...
Because most of animal viruses are enveloped, cytoplasmic entry these via fusion with cellular membrane initiates their invasion. However, the strategies in which host cells counteract such incompletely understood. Pore-forming toxin aerolysin-like proteins (ALPs) exist throughout kingdom, but functions mostly unknown. In this study, we report that βγ-crystallin fused protein and trefoil factor complex (βγ-CAT), an ALP from frog Bombina maxima, directly blocks enveloped virus invasion by...