A5019128957- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Cardiomyopathy and Myosin Studies
- Cellular Mechanics and Interactions
- Muscle Physiology and Disorders
- Bacterial Genetics and Biotechnology
- RNA Interference and Gene Delivery
- Antimicrobial Resistance in Staphylococcus
Centre National de la Recherche Scientifique
2015-2017
Université Paris Sciences et Lettres
2017
Institut Curie
2015-2017
Universidad de Sevilla
2017
Institut National de la Recherche Agronomique
2015
Sorbonne Université
2015
Myosin X has features not found in other myosins. Its structure must underlie its unique ability to generate filopodia, which are essential for neuritogenesis, wound healing, cancer metastasis and some pathogenic infections. By determining high-resolution structures of key components this motor, characterizing the vitro behaviour native dimer, we identify that explain myosin dimer behaviour. Single-molecule studies demonstrate a moves on actin bundles with higher velocities takes larger...
G-quadruplexes (G4) are polymorphic four-stranded structures formed by certain G-rich nucleic acids in vitro, but the sequence and structural features dictating their formation function vivo remains uncertain. Here we report a structure-function analysis of complex hCEB1 G4-forming sequence. We isolated four G4 conformations all which bear unusual features: Form 1 bears V-shaped loop snapback guanine; 2 contains terminal G-triad; 3 zero-nucleotide loop; 4 is monomer or an interlocked dimer....
Lactococcus lactis, a lactic acid bacterium traditionally used to ferment milk and manufacture cheeses, is also, in the biotechnology field, an interesting host produce proteins of medical interest, as it "Generally Recognized As Safe". Furthermore, L. lactis naturally secretes only one major endogenous protein (Usp45), secretion heterologous this species facilitates their purification from protein-poor culture medium. Here, we developed optimized production obtain high quality, both...