A5012261150- Lung Cancer Treatments and Mutations
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Radiopharmaceutical Chemistry and Applications
- Chemical Reactions and Mechanisms
- Advanced Chemical Physics Studies
- HER2/EGFR in Cancer Research
- Plasmonic and Surface Plasmon Research
- RNA modifications and cancer
- Photochemistry and Electron Transfer Studies
- Selenium in Biological Systems
- Cancer, Hypoxia, and Metabolism
- Gold and Silver Nanoparticles Synthesis and Applications
- GaN-based semiconductor devices and materials
- Cancer therapeutics and mechanisms
- Cyclopropane Reaction Mechanisms
- Optical Coatings and Gratings
- Photonic and Optical Devices
- Animal Genetics and Reproduction
- Orbital Angular Momentum in Optics
- Click Chemistry and Applications
- Colorectal Cancer Treatments and Studies
- Medical Imaging Techniques and Applications
- Near-Field Optical Microscopy
- Radical Photochemical Reactions
Qingdao Agricultural University
2021-2025
Xinxiang Medical University
2014-2025
Shenzhen Bay Laboratory
2024
Northwest A&F University
2015-2024
China Three Gorges University
2024
China Energy Engineering Corporation (China)
2024
National Institute of Metrology
2016-2023
Henan University of Technology
2023
Hiroshima University
2018-2021
Nanjing Medical University
2020
Patients with non-small cell lung cancer (NSCLC) activating EGF receptor (EGFR) mutations initially respond to first-generation reversible EGFR tyrosine kinase inhibitors. However, clinical efficacy is limited by acquired resistance, frequently driven the EGFR(T790M) mutation. CO-1686 a novel, irreversible, and orally delivered inhibitor that specifically targets mutant forms of EGFR, including T790M, while exhibiting minimal activity toward wild-type (WT) receptor. Oral administration as...
Targeted therapies that suppress B cell receptor (BCR) signaling have emerged as promising agents in autoimmune disease and malignancies. Bruton's tyrosine kinase (Btk) plays a crucial role development activation through the BCR pathway represents new target for diseases characterized by inappropriate activity. <i>N</i>-(3-(5-fluoro-2-(4-(2-methoxyethoxy)phenylamino)pyrimidin-4-ylamino)phenyl)acrylamide (CC-292) is highly selective, covalent Btk inhibitor sensitive quantitative assay...
Due to oxidative damage and mitochondrial dysfunction, boar semen cryopreservation remains a significant challenge. This study investigates the effects of pyrroloquinoline quinone (PQQ), mitochondrial-targeted antioxidant, on post-thaw sperm quality during cryopreservation. Boar was diluted in freezing extender containing different concentrations PQQ (0, 10, 100, 1000, 10,000 nM). After freezing-thawing, motility, viability, acrosome integrity, activity, adenosine triphosphate (ATP) levels,...
CBS-QB3 enthalpies of reaction have been computed for the conjugate additions MeSH to six α,β-unsaturated ketones. Compared with addition methyl vinyl ketone, becomes 1-3 kcal mol(-1) less exothermic when an α-Me, β-Me, or β-Ph substituent is present on C=C bond. The lower exothermicity substituted enones occurs because reactant stabilized more by hyperconjugation conjugation than product branching. Substituent effects activation energies rate-determining step thiol (reaction enone MeS(-))...
PI3Kα has been identified as an oncogene in human tumors. By use of rational drug design, a targeted covalent inhibitor 3 (CNX-1351) was created that potently and specifically inhibits PI3Kα. We demonstrate, using mass spectrometry X-ray crystallography, the selective covalently modifies on cysteine 862 (C862), amino acid unique to α isoform, PI3Kβ, -γ, -δ are not modified. is able (EC(50) < 100 nM) inhibit signaling PI3Kα-dependent cancer cell lines, this leads potent antiproliferative...
Mammalian sperm is highly susceptible to the reactive oxygen species (ROS) stress caused by biochemical and physical modifications during cryopreservation process. 5<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:msup><mml:mrow/><mml:mrow><mml:mo>′</mml:mo></mml:mrow></mml:msup></mml:math>AMP-activated protein kinase (AMPK) involved in regulating both cell metabolism cellular redox status. The aim of present study was investigate whether resveratrol protects boar...
Sperm motility patterns are continuously changed after ejaculation to fertilization in the female tract. Hyperactivated is induced with high glucose medium vitro or oviduct fluids vivo, whereas sperm maintain linear seminal plasma uterine containing low glucose. Therefore, it estimated that dependent on energy sources, and mitochondrial oxidative phosphorylation activated produce ATP condition. To elucidate these hypotheses, boar was incubated different conditions transcription translation...
The process of cryopreservation results in high concentration reactive oxygen species which is detrimental to spermatozoa. aim this study was investigate whether addition vitamin E analogue freezing extender can facilitate the cryosurvival spermatozoa rabbits, and how protects against damages during preservation. Freshly ejaculated semen diluted with Tris-citrate-glucose supplemented different concentrations Trolox (a analogue). level radical (ROS) that exposed significantly lower than...
tRNA-derived small RNAs (tsRNAs) from spermatozoa could act as acquired epigenetic factors and contribute to offspring phenotypes. However, the roles of specific tsRNAs in early embryo development remain be elucidated. Here, using pigs a research model, we probed tsRNA dynamics during spermatogenesis sperm maturation demonstrated delivery semen-derived exosomes spermatozoa. By microinjection antisense sequences into vitro fertilized oocytes subsequent single-cell RNA-seq embryos, identified...
Spermatogonial stem cells (SSCs) are the basis of spermatogenesis, and any damage to SSCs may result in spermatogenic disorder male infertility. Chromium (Cr) (VI) is a proven toxin, mutagen, carcinogen, perpetually detrimental environmental organisms due its intricate enduring detoxification process vivo . Despite this, deleterious effects Cr on underlying mechanisms remain poorly understood. In this study, we identified that impaired reproductive system mouse testes induced mitochondrial...
Cryopreservation generates a substantial quantity of ROS in semen, leading to decline sperm quality and fertilization capacity. The objective this study was investigate the effects resveratrol its optimal concentration on ram after cryopreservation. Ram semen diluted with freezing medium containing different concentrations (0, 25, 50, 75, 100 μM). After thawing, various parameters such as total motility, progressive acrosome integrity, plasma membrane mitochondrial potential, glutathione...
Sperm motility is an important factor in the migration of sperm from uterus to oviduct. During preservation vitro, generates excessive ROS that damages its function. This study aims investigate whether addition pyrroloquinoline quinone (PQQ) diluted medium could improve chilled ram quality, and then elucidates mechanism. Ram semen was with Tris-citric acid-glucose (TCG) containing different doses PQQ (0 nM, 10 100 1000 10,000 nM), stored at 4 °C. patterns, plasma membrane integrity, acrosome...
Pentalene (1) is generated for the first time in argon matrices by photocleavage of corresponding dimer (2). It found that cleavage occurs two distinct steps, which leads presumably to a diradical. 1 characterized its electronic and vibrational absorption spectra are assigned interpreted with reference different quantum chemical calculations. These show excited states pentalene involve doubly configuration had been ignored previous discussion structure. Due antiaromatic nature pentalene,...
Laser flash photolysis (LFP, Nd:YAG laser, 35 ps, 266 nm, 10 mJ) of phenyl azide (PA) releases open-shell singlet phenylnitrene (1PN). 1PN has a sharp absorption band at 350 nm and very weak 540 nm. These bands are assigned as nz−ny mixture 52% π−nx 28% nx−π* transitions, respectively, on the basis CASSCF CASPT2 calculations. A calculation spectrum triplet (3PN) is in good agreement with experimental spectrum. 3PN 308 broad, structured 370 π−π transitions The low intensity, long wavelength...
A multilayered metallic M-shaped nano-grating is proposed to enhance the internal quantum efficiency, light extraction efficiency and surface-plasmon (SP) of gallium nitride-based emitting diodes. This structure fabricated by low-cost nano-imprint lithography. The suitable grating based on quasi-symmetrical-waveguide has a high transmission in visible region. properties SP mode Purcell effect this type LED investigated. experimental results demonstrate that its peak photoluminescence...
Patients with non-small cell lung carcinoma (NSCLC) activating mutations in epidermal growth factor receptor (EGFR) initially respond well to the EGFR inhibitors erlotinib and gefitinib. However, all patients relapse because of emergence drug-resistant mutations, T790M accounting for approximately 60% resistance. Second-generation irreversible are effective against vitro, but retain affinity wild-type (EGFR(WT)). These have not provided compelling clinical benefit T790M-positive patients,...
Abstract Cascaded optical field enhancement (CFE) can be realized in some specially designed multiscale plasmonic nanostructures, which the generation of extremely strong fields at nanoscale volume is crucial for many applications, example, surface-enhanced Raman spectroscopy (SERS). In this paper, we propose a strategy realizing high-quality nanoparticle-in-cavity (PIC) nanoantenna array, coupling between nanoparticle (NP) dark mode with high-order nanocavity bright produce Fano resonance...
Abstract Hepatocellular carcinoma (HCC) is the second leading cause of cancer related-death. As a major common regulator numerous cancer-driving pathways and unique therapeutic target, prolyl isomerase Pin1 overexpressed in majority HCCs, whereas mechanism underlying overexpression remains elusive. Here we find that miR-140-5p inhibits HCC by directly targeting to block multiple pathways. Bioinformatics analysis, miRNA binding functional assays identify interacts with 3′UTR translation....
: It takes several hours for mammalian sperm to migrate from the ejaculation or insemination site fertilization in female reproductive tract which glucose, amino acids, and fatty acids are regarded as primary substrates ATP generation. The present study was designed investigate whether oleic acid palmitic were beneficial boar vitro; if yes, elucidate mechanism that regulates motility. Therefore, levels of acid, motility, membrane integrity, acrosome apoptosis evaluated. Moreover, enzymes...
Abstract Sperm are susceptible to excessive reactive oxygen species (ROS). Spermine and spermidine secreted in large amounts by the prostate potent natural free radical scavengers protect cells against redox disorder. Thus, we used boar sperm as a model study polyamines uptake elucidate whether protected from ROS stress. Seven mature fertile Duroc boars (aged 15 30 mo) were this study. In experiment 1, spermine (3.6 ± 0.3 3.3 0.2 mmol/L, respectively) abundant seminal plasma, content of...
Sperm cryopreservation contributes to the extensive utilization of artificial insemination (AI) in daily livestock industry. However, due presence few sperm with good biological function post-thaw goat sperm, its use has been limited for AI purposes. Hence, improvement focus many research studies. This study aimed investigate effects proline supplementation freezing medium on sperm. The semen was cryopreserved different concentrations (0, 0.5, 1, 2 and 4 mM). motility patterns, membrane...