A5102023779- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Reproductive Biology and Fertility
- Protein Structure and Dynamics
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- Metabolism and Genetic Disorders
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Chemical Synthesis and Analysis
- Sphingolipid Metabolism and Signaling
- Sperm and Testicular Function
- Cancer-related gene regulation
- Viral Infectious Diseases and Gene Expression in Insects
- Sulfur-Based Synthesis Techniques
- Rabies epidemiology and control
- Cancer Mechanisms and Therapy
- RNA Research and Splicing
- Per- and polyfluoroalkyl substances research
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Click Chemistry and Applications
- Natural Fiber Reinforced Composites
Yantai University
2025
Northwest A&F University
2015-2025
Qingdao Institute of Bioenergy and Bioprocess Technology
2021-2024
Chinese Academy of Sciences
2021-2024
Qinghai New Energy (China)
2024
Powerchina Huadong Engineering Corporation (China)
2024
PowerChina (China)
2024
Northwestern Polytechnical University
2022-2024
Nanjing Tech University
2024
Nanjing Medical University
2021-2023
Abstract Recent advances in the study of CRISPR/Cas9 system have provided a precise and versatile approach for genome editing various species. However, applicability efficiency this method large animal models, such as goat, not been extensively studied. Here, by co-injection one-cell stage embryos with Cas9 mRNA sgRNAs targeting two functional genes ( MSTN FGF5 ), we successfully produced gene-modified goats either one or both disrupted. The cultured primary fibroblasts was high 60%, while...
An iron(III)-mediated nucleophilic cascade cyclization of N-propiolyl enamides with various diselenides was developed, which provides an efficient way to construct seleno-heterocycles. A mechanism study shows that the process involves selective addition C≡C bond generating a seleniranium ion, followed by intramolecular attack enaminic carbon tertiary enamide. Utilizing this protocol, variety 3-seleno-2-pyridones were successfully synthesized featuring good functional group compatibility and...
A Mn(III)-mediated radical cascade cyclization of N-propargyl enamides with sodium sulfinates was developed. Mechanistic studies indicated that this process is mainly composed the chemoselective addition sulfonyl to C≡C bond and regioselective intramolecular 6-endo-trig cyclization. This protocol provided a powerful method for divergent synthesis diverse polysubstituted meta-sulfonylpyridines or 1,2-dihydropyridines just by varying solvent temperature featuring good functional group...
tRNA-derived small RNAs (tsRNAs) from spermatozoa could act as acquired epigenetic factors and contribute to offspring phenotypes. However, the roles of specific tsRNAs in early embryo development remain be elucidated. Here, using pigs a research model, we probed tsRNA dynamics during spermatogenesis sperm maturation demonstrated delivery semen-derived exosomes spermatozoa. By microinjection antisense sequences into vitro fertilized oocytes subsequent single-cell RNA-seq embryos, identified...
Critical mutations of mitochondrial DNA (mtDNA) generally lead to maternally inheritable diseases that affect multiple organs and systems; however, it was difficult alter mtDNA in mammalian cells intervene or cure disorders. Recently, the discovery DddA-derived cytosine base editor (DdCBE) enabled precise manipulation mtDNA. To test its feasibility for vivo use, we selected several sites mouse as DdCBE targets resemble human pathogenic G-to-A mutations. The efficiency DdCBE-mediated editing...
Conformational dynamics is important for protein functions, many of which are performed in cells. How the intracellular environment may affect conformational largely unknown. Here, loop studied a model Escherichia coli cells by using nuclear magnetic resonance (NMR) spectroscopy. The weak interactions between and surrounding macromolecules hinder rotational diffusion, extends dynamic detection timescale up to microseconds NMR spin relaxation method. picosecond microsecond confirmed...
DNA has emerged as an appealing material for information storage due to its great density and durability. Random reading rewriting are essential tasks practical large-scale data storage. However, they currently difficult implement simultaneously in a single DNA-based system, strongly limiting their practicability. Here, "Cell Disk" system is presented, achieving high-density vivo that enables both random rewriting. In this each yeast cell used chamber store information, similar "disk block"...
Spermatogonial stem cells (SSCs) provide the foundation for spermatogenesis and fertility throughout adult life of a male. Genetic manipulations SSCs combined with germ cell transplantation present novel approach gene therapy production genetically modified animals. However, rarity within mammalian testes remains an impediment to related applications, making in vitro expansion prerequisite. Nevertheless, long-term culture systems from large animals have not been established yet. In this...
Abstract Targeted codelivery and controlled release of drug/siRNA (small interfering RNA) in a safe effective vehicle hold great promises for overcoming drug resistance optimal efficacy cancer treatment; however, rational design preparation such vehicles remain critical challenge. Thus, glyco‐nanofibers (GNFs) are fabricated via supermolecular assembly polyanionic siRNA cationic vesicles to simultaneously deliver doxorubicin hydrochloride (DOX) vitro vivo. The created through self‐assembly...
Abstract Objectives Accumulating evidences show that the regulatory network of m 6 A modification is essential for mammalian spermatogenesis. However, as an reader, roles YTHDF2 remain enigmatic due to lack a proper model. Here, we employed germ cell conditional knockout mouse model and explored function in Materials methods Ythdf2 mice were obtained by crossing ‐floxed with Vasa ‐ Cre Stra8 mice. Haematoxylin eosin (HE) staining, immunofluorescent staining Western blotting used phenotyping....
Infertility caused by male factors is routinely diagnosed assessing traditional semen parameters. Growing evidence has indicated that the tsRNAs carried in sperm act as epigenetic and potential biomarkers for assessment of quality. We recently demonstrated tRNA Gln-TTG derived small RNAs played notable roles first cleavage a porcine embryo. However, function human diagnostic biomarker its role early embryo development remains unclear. In this study, we found were highly associated with By...
High-throughput genetic screens are powerful methods to interrogate gene function on a genome-wide scale and identify genes responsible certain stresses. Here, we developed piggyBac strategy deliver pooled sgRNA libraries stably into cell lines. We used this conduct screen based clustered regularly interspaced short palindromic repeat technology (CRISPR)-Cas9 in Bombyx mori cells. first constructed single guide RNA (sgRNA) library containing 94,000 sgRNAs, which targeted 16,571...
Functionalized pyridines and 1,2‐dihydropyridines are crucial organic entities in natural products, materials, drugs catalysts. Despite progress, a facile eco‐friendly synthesis remains desirable so far. In this context, we disclose novel visible light‐promoted radical cascade sulfonylation‐cyclization‐aromatization of N‐propargyl enamides with selenosulfonates. This approach avoids the use metal‐ or photocatalyst, bases external oxidants, marking significant advance sustainable green...
A copper(II)-catalyzed radical cascade reaction of N-propargyl enamides with sulfonyl hydrazides was developed, which provided an unprecedented method for the direct synthesis meta-sulfonylpyridines in high efficiency good functional group compatibility and simple operation. Mechanistic studies indicated that this tandem process might be composed addition, intramolecular cyclization oxidative aromatization.
Abstract Background Heat stress (HS) poses a significant threat to male goat reproduction. Sertoli cells (SCs) provide both structural and nutritional support necessary for germ cells. HS induces physiological biochemical changes in SCs. Nevertheless, the molecular mechanisms involved are still not fully understood. Melatonin is classic antioxidant that can alleviate HS-induced reproductive damage. However, underlying by which melatonin mitigates damage testicular SCs remain unclear require...