A5102223693- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Olfactory and Sensory Function Studies
- Neuroendocrine regulation and behavior
- Pluripotent Stem Cells Research
- Xenotransplantation and immune response
- High Altitude and Hypoxia
- Virus-based gene therapy research
- Nerve injury and regeneration
- Viral Infectious Diseases and Gene Expression in Insects
- Isotope Analysis in Ecology
- Insect symbiosis and bacterial influences
- Pacific and Southeast Asian Studies
- Winter Sports Injuries and Performance
- Nerve Injury and Rehabilitation
- Chromosomal and Genetic Variations
- Cellular and Composite Structures
- Pain Mechanisms and Treatments
- Plant Virus Research Studies
- Hair Growth and Disorders
- Spinal Cord Injury Research
- Image and Video Quality Assessment
- Electronic Packaging and Soldering Technologies
- Soil and Water Nutrient Dynamics
- Eicosanoids and Hypertension Pharmacology
Peking University First Hospital
2025
Peking University
2025
Jilin Agricultural University
2025
China Medical University
2024-2025
Guilin Medical University
2018-2024
Tsinghua University
2024
BOE Technology Group (China)
2021
Institute of Computing Technology
2021
Northwest A&F University
2018
ShanghaiTech University
2016-2018
Abstract Recent advances in the study of CRISPR/Cas9 system have provided a precise and versatile approach for genome editing various species. However, applicability efficiency this method large animal models, such as goat, not been extensively studied. Here, by co-injection one-cell stage embryos with Cas9 mRNA sgRNAs targeting two functional genes ( MSTN FGF5 ), we successfully produced gene-modified goats either one or both disrupted. The cultured primary fibroblasts was high 60%, while...
Abstract The CRISPR/Cas9 system provides a flexible approach for genome engineering of genetic loci. Here, we successfully achieved precise gene targeting in sheep by co-injecting one-cell-stage embryos with Cas9 mRNA and RNA guides three genes ( MSTN , ASIP BCO2 ). We carefully examined the sgRNAs:Cas9-mediated effects injected embryos, somatic tissues, as well gonads via cloning sequencing. efficiencies these were within range 27–33% generated lambs that simultaneously was 5.6%, which...
Dystrophinopathy, including Duchenne muscle dystrophy (DMD) and Becker (BMD) is an incurable X-linked hereditary caused by a mutation in the
Precision genetic engineering accelerates the improvement of livestock for agriculture and biomedicine. We have recently reported our success in producing gene-modified goats using CRISPR/Cas9 system through microinjection Cas9 mRNA sgRNAs targeting MSTN FGF5 genes goat embryos. By investigating influence gene modification on phenotypes Cas9-mediated goats, we herein demonstrate that utility this approach involving disruption results increased number second hair follicles enhanced fiber...
The induced pluripotent stem cells (iPSCs), derived by ectopic expression of reprogramming factors in somatic cells, can potentially provide unlimited autologous for regenerative medicine. In theory, the from patient iPSCs should be immune tolerant host without any rejections. However, our recent studies have found that even syngeneic iPSC-derived immunogenic hosts using a teratoma transplantation model (Nature 474:212-215, 2011). Recently two research groups differentiated into different...
Since its emergence, CRISPR/Cas9-mediated base editors (BEs) with cytosine deaminase activity have been used to precisely and efficiently introduce single-base mutations in genomes, including those of human cells, mice, crop species. Most production traits livestock are induced by point mutations, genome editing using BEs without homology-directed repair double-strand breaks can directly alter single nucleotides. The p.96R>C variant Suppressor cytokine signaling 2 (SOCS2) has profound...
Abstract Objective . Artificial nerve scaffolds composed of polymers have attracted great attention as an alternative for autologous grafts recently. Due to their poor bioactivity, satisfactory repair could not be achieved. To solve this problem, we introduced extracellular matrix (ECM) optimize the materials. Approach. In study, ECM extracted from porcine nerves was mixed with Poly(L-Lactide-co- ϵ -caprolactone) (PLCL), and innovative PLCL/ECM conduits were prepared by electrostatic...
Since its emergence, the clustered regularly interspaced short palindromic repeat (CRISPR)–CRISPR-associated (Cas) 9 system has been increasingly used to generate animals for economically important traits. However, most CRISPR/Cas9 applications have focused on non-homologous end joining, which results in base deletions and insertions, leading a functional knockout of targeted gene. The Booroola fecundity gene (FecBB) mutation (p.Q249R) bone morphogenetic protein receptor type 1B (BMPR1B)...
The ability to alter single bases without homology directed repair (HDR) of double-strand breaks provides a potential solution for editing livestock genomes economic traits, which are often multigenic. Progress toward multiplex in large animals has been hampered by the costly inefficiencies HDR via microinjection vitro manipulated embryos. Here, we designed sgRNAs induce nonsense codons (C-to-T transitions) at four target sites caprine FGF5, is crucial regulator hair length mammals. Initial...
Abstract Base editing has the potential to improve important economic traits in agriculture and can precisely convert single nucleotides DNA or RNA sequences into minimal double-strand breaks (DSB). Adenine base editors (ABE) have recently emerged as a tool for conversion of targeted A:T G:C, but not yet been used sheep. ABEmax is one latest versions ABE, which consists catalytically-impaired nuclease laboratory-evolved DNA-adenosine deaminase. The Booroola fecundity ( FecB B ) mutation...
Adopting an optimal cropping regime is crucial for sustainable soil use. However, how different regimes impact phosphorus (P) availability and the underlying mechanism remain unclear. Here, a 10-year field experiment was performed to examine influence of regimes, including maize–soybean rotation (MSR), continuous maize (CMC), farmland fallow (FALL), under unfertilized fertilized conditions in Northeast China. The P forms were analyzed using chemical fractionation solution phosphorus-31...
Background: Brain and central nervous system (CNS) cancers present significant health challenges globally, characterized by increasing incidence mortality rates. This study utilizes data from the Global Burden of Disease (GBD) 2021 to analyze trends project future burdens. Methods: We calculated age-standardized rates (ASRs) incidence, mortality, disability-adjusted life years (DALYs) for brain CNS 1990 2021. Trends were analyzed using estimated annual percentage change, projections made...
α1,3-Galactosyltransferase (GGTA1) is essential for the biosynthesis of glycoproteins and therefore a simple effective target disrupting expression galactose α-1,3-galactose epitopes, which mediate hyperacute rejection (HAR) in xenotransplantation. Miniature pigs are considered to have greatest potential as xenotransplantation donors. A GGTA1-knockout (GTKO) miniature pig might mitigate or prevent HAR xenotransplantation.Transcription activator-like effector nucleases (TALENs) were designed...
Laron syndrome is an autosomal disease resulting from mutations in the growth hormone receptor (GHR) gene. The only therapeutic treatment for recombinant insulin-like factor I (IGF-I), which has been shown to have various side effects. improved models are important better understanding pathogenesis of and developing corresponding therapeutics. Pigs become attractive biomedical human condition due similarities anatomy, physiology, metabolism relative humans, could serve as appropriate model syndrome.
The plateau pika (Ochotona curzonia) has adapted to high-altitude hypoxia during evolution. Higher microvessel density in specific tissues and a blunted hypoxic pulmonary vasoconstriction response are the critical components of this adaptation. VEGF, vascular endothelial growth factor, proved be key regulator angiogenesis tissue play an important role vasodilation. However, VEGF adaptation remains unknown. In study, we cloned cDNAs for VEGF165 VEGF189 examined their expression pikas...
Activation of human immune T-cells by swine leukocyte antigens class I (SLA-I) and II (SLA-II) leads to xenograft destruction. Here, we generated the GGTA1, B2M, CIITA (GBC) triple-gene-modified Diannan miniature pigs, analyzed transcriptome GBC-modified peripheral blood mononuclear cells (PBMCs) in pig's spleen, investigated their effectiveness anti-immunological rejection. A total six cloned piglets were successfully using somatic cell nuclear transfer, one them carrying heterozygous...
Applying high dynamic range(HDR) to mini‐LED backlight is the current research interest of display. The partition control technology for mini LED adopted improve image contrast, however it can not avoid introduction halo, which affects quality and needs be solved. Therefore, this article firstly proposes a method evaluate halo; Then we studied brightness distribution halo from perspective module. We also put forward some factors that affect size halo. It hoped design partitions guided through study
Neuropathic pain (NP) after spinal cord injury (SCI-evoked NP) is clinically challenging; the underlying mechanisms are not fully understood, leading to a lack of promising treatment options. NP occurs in only subset patients with SCI. The injured exhibits series histopathological changes, and complement system has been shown play an important role these processes. In addition, NMDA receptor subunit 2B (NR2B) involved development maintenance NP. This preliminary study was performed...