A5049842723- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Click Chemistry and Applications
- Virus-based gene therapy research
- Chemical Synthesis and Analysis
- Supramolecular Self-Assembly in Materials
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Graphene and Nanomaterials Applications
- Nanowire Synthesis and Applications
- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- RNA Research and Splicing
- Pneumonia and Respiratory Infections
- Cancer Immunotherapy and Biomarkers
- interferon and immune responses
- Neonatal Respiratory Health Research
- Cellular Mechanics and Interactions
- Advanced NMR Techniques and Applications
- RNA modifications and cancer
- Ion Channels and Receptors
- Electrochemical sensors and biosensors
- Respiratory viral infections research
Tsinghua University
2006-2025
Center for Life Sciences
2024
Henan Agricultural University
2024
Ningbo University
2021-2022
Beijing Technology and Business University
2022
Jiangnan University
2022
Xi'an Honghui Hospital
2021
Xi'an Jiaotong University
2021
Henan Provincial People's Hospital
2017-2018
Scripps Research Institute
2013-2017
Significance Chimeric antigen receptor T (CAR-T) cell therapy has produced promising results in clinical trials but been challenged by the inability to control engineered cells once infused into patient. Here we present a generalizable method of controlling CAR-T using peptide-engrafted antibody-based molecular switches that act as bridge between target and cell. We show specific for CD19 govern activity, tissue-homing, cytokine release, phenotype switchable dose-titratable manner xenograft...
Fusarium species produce a secondary metabolite known as T-2 toxin, which is the primary and most harmful toxin found in type A trichothecenes. widely food grain-based animal feed endangers health of both humans animals. exposure animals occurs primarily through administration; therefore, first organ that targets gut. In this overview, research progress, toxicity mechanism, detoxification were reviewed, future directions proposed. damages intestinal mucosa destroys structure barrier...
Abstract Chimeric antigen receptor T (CAR‐T) cells have demonstrated promising results against hematological malignancies, but encountered significant challenges in translation to solid tumors. To overcome these hurdles, we developed a switchable CAR‐T cell platform which the activity of engineered is controlled by dosage an antibody‐based switch. Herein, apply this approach Her2‐expressing breast cancers engineering switch molecules through site‐specific incorporation FITC or grafting...
The aim of this study was to evaluate water-holding capacity, texture, rheological properties, interactions and microstructure myofibrillar proteins (MP) gel with the addition fibrin during heat-induced process. Compared control, whiteness, capacity hardness MP 2% increased by 9.70%, 79.36%, 323.07%, respectively. Storage modulus loss values in group were higher than those control. conformation random coil gradually transformed β-sheet β-turn increase content; nonspecific association...
The activation of multiple Pattern Recognition Receptors (PRRs) has been demonstrated to trigger inflammatory responses and coordinate the host's adaptive immunity during pathogen infections. use PRR agonists as vaccine adjuvants reported synergistically induce specific humoral cellular immune responses. However, incorporating increases complexity design manufacturing. In this study, we discovered a polymer that can activate both Toll-like receptor (TLR) pathway cyclic GMP-AMP synthase...
Targeted protein delivery with nanocarriers holds significant potential to enhance therapeutic outcomes by precisely directing proteins specific organs or tissues. However, the complex interactions between and biological environment pose considerable challenges in designing effective targeted vehicles. In this study, we address challenge leveraging DNA-barcoded high-throughput screening. We construct a nanocapsule library via in-situ polymerization, incorporating various monomers create...
Enzyme-responsive protein nanocapsules are synthesized to release their cargoes in response specific enzymes secreted certain cellular events with both specificity and a controlled rate by composition tuning. The unique nanocapsule structures protect the encapsulated proteins during hydrogel synthesis, providing new direction for responsive delivery according or local environment. Detailed facts of importance specialist readers published as "Supporting Information". Such documents...
Nanocapsules of organophosphorous hydrolase with enhanced enzyme activity and stability are prepared via in situ polymerization, providing a novel class nanoparticles for the decontamination detoxification organophosphates such as chemical warfare agents pesticides. Using nanocapsules building blocks, bioactive nanocomposites also fabricated, enabling their use organophosphate protection. As service to our authors readers, this journal provides supporting information supplied by authors....
Myotonic dystrophy type 1 (DM1) is an inherited dominant muscular caused by expanded CTG·CAG triplet repeats in the 3' untranslated region of DMPK1 gene, which produces a toxic gain-of-function CUG RNA. It has been shown that severity disease symptoms, age onset and progression are related to length repeats. However, mechanism(s) triplet-repeat instability not fully understood. Herein, induced pluripotent stem cells (iPSCs) were generated from DM1 Huntington's patient fibroblasts. We...
Antibody–drug conjugate (ADC) and immune checkpoint blockade (ICB) offer promising approaches for cancer treatment. Here, we describe an ADC constructed by conjugating anti-PD-L1 THIOMAB with a bifunctional immunomodulator D18 via redox-cleavable linker. The resulting HE-S2 not only triggers potent antitumor response blocking the PD-1/PD-L1 interaction activating Toll-like receptor 7/8 (TLR7/8) signaling pathway but also upregulates its targeted PD-L1 expression epigenetic regulation and/or...
Currently, the clinical application of protein/peptide therapeutics is mainly limited to modulation diseases in extracellular spaces. Intracellular targets are hardly accessed, owing largely endosomal entrapment internalized proteins/peptides. Here, we report a strategy design and construct peptides that enable endosome-to-cytosol delivery based on an extension "histidine switch" principle. By substituting Arg/Lys residues cationic cell-penetrating (CPPs) with histidine, obtained...
Bioluminescence, due to its high sensitivity, has been exploited in various analytical and imaging applications. In this work, we report a highly stable, cell-transductable, wavelength-tunable bioluminescence system achieved with an elegant simple design. Using aqueous situ polymerization on bioluminescent enzyme anchored polymerizable vinyl groups, obtained nanosized core−shell nanocapsules the as core cross-linked thin polymer net shell. These possess greatly enhanced stability, retained...
Growth factors are of great potential in regenerative medicine. However, their clinical applications largely limited by the short vivo half-lives and narrow therapeutic window. Thus, a robust controlled release system remains an unmet medical need for growth-factor-based therapies. In this research, nanoscale (degradable protein nanocapsule) is established via situ polymerization on growth factor. The rate can be finely tuned engineering surface polymer composition. Improved outcomes...
Abstract A hypoxia‐responsive fluorescence probe of amphiphilic PEGylated azobenzene caged tetraphenylethene (TPE) for tumor cell imaging is reported; it possesses excellent solubility in aqueous medium due to the easy formation micelles by self‐assembly. The resonance energy transfer (FRET) process ensures that azobenene AIE fluorogen quenched efficiently. When cultured with cells, azo‐bond reduced under hypoxia conditions and recovers dramatically. Besides using UV light, NIR light can...
Photoirradiation of the PC4AP linker generates an active intermediate that reacts intramolecularly with a primary amine on carrier peptide/protein, leading to rapid release drug without generating any toxic side products.
Intracellular delivery of biologicals such as peptides, proteins, and nucleic acids presents a great opportunity for innovative therapeutics. However, the endosome entrapment remains major bottleneck in intracellular biomacromolecules, largely limiting their therapeutic potential. Here, we converted cell-penetrating peptide (CPP), low molecular weight protamine (LMWP), to endosomal escape peptides (EEPs) by masking LMWP with pH-responsive counter-ionic peptide. The resulting masked CPPs...
Protein-polymer conjugates were investigated as nonviral gene delivery vectors. BSA-poly(dimethylamino) ethyl methacrylate (PDMA) nanoparticles (nBSA) synthesized using in situ atom transfer radical polymerization (in ATRP) and BSA a macroinitiator. The diameter charge of nBSA was function the ATRP reaction time ranged from 5 to 15 nm +8.9 +22.5, respectively. able condense plasmid DNA (pDNA) form polyplexes with an average 50 nm. nBSA/pDNA transfected cells similar efficiencies or better...
Simultaneous delivery of multiple genes and proteins (e.g., transcription factors; TFs) is an emerging issue surrounding therapeutic research due to their ability regulate cellular circuitry. Current gene protein strategies, however, are based on slow batch synthesis, which ineffective, poorly controlled, incapable simultaneous both with synergistic functions. Consequently, advances in this field have been limited vitro studies. Here, by integrating microfluidic technologies a supramolecular...
The activation of stimulator interferon genes (STING) signaling induces the production type I interferons (IFNs), which play critical roles in protective innate immunity for host to defend against viral infections. Therefore, achieving sustained or enhanced STING could become an antiviral immune strategy with potential broad-spectrum activities. Here, we discovered that various clinically used microtubule-destabilizing agents (MDAs) treatment cancer showed a synergistic effect response....