A5056615400- Biosensors and Analytical Detection
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- Advanced biosensing and bioanalysis techniques
- Pancreatitis Pathology and Treatment
- Electrowetting and Microfluidic Technologies
- Pancreatic and Hepatic Oncology Research
- Liver Disease Diagnosis and Treatment
- MicroRNA in disease regulation
- Animal Virus Infections Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Reproductive Biology and Fertility
- RNA Interference and Gene Delivery
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Microfluidic and Bio-sensing Technologies
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Animal Genetics and Reproduction
- Herpesvirus Infections and Treatments
- Antibiotics Pharmacokinetics and Efficacy
- Microbial infections and disease research
- Circular RNAs in diseases
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
Jinan Central Hospital
2024-2025
Shandong First Medical University
2024-2025
Harvard University
2025
Beth Israel Deaconess Medical Center
2025
Johns Hopkins Medicine
2021-2024
Johns Hopkins University
2006-2024
University of Hong Kong
2024
Hangzhou Normal University
2024
Chinese Academy of Sciences
2024
University of Science and Technology of China
2024
Abstract Lipid nanoparticles (LNPs) are effective vehicles to deliver mRNA vaccines and therapeutics. It has been challenging assess packaging characteristics in LNPs, including payload distribution capacity, which critical understanding structure-property-function relationships for further carrier development. Here, we report a method based on the multi-laser cylindrical illumination confocal spectroscopy (CICS) technique examine lipid contents LNP formulations at single-nanoparticle level....
A novel organic field effect transistor (OFET)‐based biosensor is described for label‐free glial fibrillary acidic protein detection. This study reports the first use of an extended solution gate structure where sensing area and semiconductor are separated, a reference electrode not needed. Different molecular weight polyethylene glycols (PEGs) mixed into bioreceptor layer to help extend Debye screening length. The drain current change significantly increased with higher PEGs, as they known...
tRNA-derived small RNAs (tsRNAs) from spermatozoa could act as acquired epigenetic factors and contribute to offspring phenotypes. However, the roles of specific tsRNAs in early embryo development remain be elucidated. Here, using pigs a research model, we probed tsRNA dynamics during spermatogenesis sperm maturation demonstrated delivery semen-derived exosomes spermatozoa. By microinjection antisense sequences into vitro fertilized oocytes subsequent single-cell RNA-seq embryos, identified...
Antimicrobial resistance is a global threat that if left unchecked could lead to 10 million annual mortalities by 2050. One factor contributing the rise of multi-drug-resistant (MDR) pathogens reliance on traditional culture-based pathogen identification (ID) and antimicrobial susceptibility testing (AST) typically takes several days. This delay objective ID AST information inform clinical decision making results in clinicians treating patients empirically often using first-line,...
Abstract Sanguinarine, a bioactive benzophenanthridine alkaloid extracted from plants of the Papaveraceae family, has shown antitumour effects in multiple cancer cells. But therapeutic and regulatory mechanisms sanguinatine gastric (GC) remain elusive. This study was aimed to investigate correlation dual‐specificity phosphatase 4 (DUSP4) expression with clinicopathologic features overall survival patients GC explore sanguinarine on tumour growth invasion cells (SGC‐7901 HGC‐27) underlying...
Abstract Hepatocellular carcinoma (HCC) is one of the most fatal cancers with common features invasion and metastasis. Recent evidence indicate that long noncoding RNA NORAD a potential oncogene significantly upregulated in several cancers. However, general biological role clinical value HCC remains unknown. Here, expression was measured 29 paired tumor paratumor tissues via quantitative real‐time polymerase chain reaction (qPCR). The effects on cell malignant were investigated...
The global threat of antibiotic resistance underscores critical but unmet needs for rapid susceptibility testing (AST) technologies. To this end, droplet microfluidic-based single-cell AST offers promise by achieving unprecedented rapidity, its potential clinical use is marred the capacity one to few conditions per device, which falls short from required scale in clinically relevant scenarios. lift scalability constraint technologies, a new cascaded microfluidic platform that can streamline...
Thoracic aortic aneurysm (TAA) is a life-threatening condition that currently lacks an effective therapeutic strategy. Phenotypic switching in vascular smooth muscle cells (VSMCs) and extracellular matrix (ECM) degradation are considered to be among the causes of TAA development. Trimethylamine N-oxide (TMAO) gut microbial metabolite has been associated with increased risk cardiovascular diseases. However, its general association remains unclear. Therefore, present study aimed assess...
PRV is a critical pathogen threatening the global swine industry. Our research fills knowledge gaps regarding prevalence, infection risk factors, spatial-temporal clustering of high gE seroprevalence, and epidemic trend seroprevalence in China recent years. These findings are valuable for clinical prevention control suggest that likely to be successfully controlled China.
Abstract Organic field‐effect transistor (OFET)‐based biosensors with antibody receptors are widely considered for protein antigen detection. To the authors' knowledge, there no comparative evaluations to date of different choices matrix polymer which antibodies attached. Herein, multiple acrylic copolymers studied as receptor layers myelin basic and its corresponding an archetypal antibody–antigen pair. Stability against washing steps capacity immobilizing on polymers device surfaces help...
Abstract Droplet microfluidic technology is becoming increasingly useful for high-throughput and high-sensitivity detection of biological biochemical reactions. Most current droplet devices function by passively discretizing a single sample subject to homogeneous or random reagent/reaction condition into tens thousands picoliter-volume droplets analysis. Despite their apparent advantages in speed throughput, these inherently lack the capability customize contents order test against multiple...
In the face of global threat from drug-resistant superbugs, there remains an unmet need for simple and accessible diagnostic tools that can perform important antibiotic susceptibility testing against pathogenic bacteria guide treatments outside centralized clinical laboratories. As a potential solution to this problem, we report herein development microwell array-based resazurin-aided colorimetric test (marcAST). At core marcAST is ready-to-use array device preassembled with custom titers...
The emergence and spread of multidrug resistant bacterial strains concomitant dwindling effective antibiotics pose worldwide healthcare challenges. To address these challenges, advanced engineering tools are developed to personalize antibiotic treatments by speeding up the diagnostics that is critical prevent misuse overuse make full use existing antibiotics. Meanwhile, it necessary investigate novel strategies. Recently, repurposing mono into combinatorial therapies has shown great...
Haemophilus parasuis (H. parasuis) is a conditional pathogen with the ability to form biofilms which can lead ineffective drug treatment and severe chronic infections resulting in significant economic losses pig industry. Currently, knowledge of biofilm formation by H. not well developed. The objective this study was investigate three-dimensional morphology perform transcriptomic analysis on cells versus planktonic forms. results showed that proteins DNA accounted for large proportion...
Many protein biomarkers are present in biofluids at a very low level but may play critical roles important biological processes. The fact that these low-abundance proteins remain largely unexplored underscores the importance of developing new tools for highly sensitive detection. Although digital enzyme-linked immunosorbent assay (ELISA) has demonstrated ultrahigh sensitivity compared with conventional ELISA, requirement specialized instruments limits accessibility and prevents widespread...
We have studied envelope protein from a donor with nonprogressive HIV-1 infection whose serum contains broadly cross-reactive, primary virus NA. DNA was extracted lymphocytes, which had been collected approximately 6 and 12 months prior to the time of collection cross-reactive serum, env genes were synthesized, cloned, expressed on pseudoviruses, phenotyped in NA assays. Two clones each point identical V3 region nucleotide sequences, utilized CCR5 but not CXCR4 for cell entry, similar...
We developed a facile PDMS-based, vacuum-assisted microfluidic device that can perform multi-step and multi-reagent digital assays.
We devised and interfaced a facile filtration-based bacteria recovery buffer exchange process with droplet microfluidics for clinical isolation-free digital detection characterization of pathogens in urine.
A green and scalable heterogeneous photocatalysis platform is efficiently established for multigram juglone production using photomicroreactors, which are at the forefront of sustainable flow chemistry.
Developing effective vaccines is of vital importance for protecting public health by preventing potential pandemics or controlling ongoing ones. However, there a threshold rapidly design and develop to prevent virus infection. Inspired the natural budding processes associated with cell membrane scission when enveloped viruses invade host cells replicate themselves, similar strategy was applied achieve virus-mimetic nanovesicles (VMVs). This loaded genetically engineered viral antigens onto...