A5102746118- Spaceflight effects on biology
- MicroRNA in disease regulation
- Bone Metabolism and Diseases
- Circular RNAs in diseases
- RNA Research and Splicing
- Cardiovascular Function and Risk Factors
- Ion channel regulation and function
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Anesthesia and Neurotoxicity Research
- Erythrocyte Function and Pathophysiology
- Ginseng Biological Effects and Applications
- Cardiac Fibrosis and Remodeling
- Mesenchymal stem cell research
- Muscle Physiology and Disorders
- Peptidase Inhibition and Analysis
- Histone Deacetylase Inhibitors Research
- Genetics, Aging, and Longevity in Model Organisms
- Signaling Pathways in Disease
- Medical and Biological Ozone Research
- Tissue Engineering and Regenerative Medicine
- Extracellular vesicles in disease
- Lipid metabolism and disorders
- Liver Disease Diagnosis and Treatment
- Acute Ischemic Stroke Management
China Astronaut Research and Training Center
2016-2025
University of Science and Technology Beijing
2023-2025
Zhejiang Lab
2023
State Key Laboratory of Space Medicine Fundamentals and Application
2011-2022
Shanghai Jiao Tong University
2022
Guangzhou University of Chinese Medicine
2021-2022
XinHua Hospital
2022
Air Force Medical University
2021
Beijing Proteome Research Center
2012
Dalian Medical University
2012
Mechanical load of the skeleton system is essential for development, growth, and maintenance bone. However, molecular mechanism by which mechanical stimuli are converted into osteogenesis bone formation remains unclear. Here we report that Piezo1, a bona fide mechanotransducer critical various biological processes, plays role in formation. Knockout Piezo1 osteoblast lineage cells disrupts osteoblasts severely impairs structure strength. Bone loss induced unloading blunted knockout mice....
MicroRNAs have an important role in bone homeostasis. However, the detailed mechanism of microRNA-mediated intercellular communication between cells remains elusive. Here, we report that osteoclasts secrete microRNA-enriched exosomes, by which miR-214 is transferred into osteoblasts to inhibit their function. In a coculture system, inhibition exosome formation and secretion prevented transportation. Exosomes specifically recognized through interaction ephrinA2 EphA2. osteoclast-specific...
Rationale: Breast cancer preferentially develops osteolytic bone metastasis, which makes patients suffer from pain, fractures and spinal cord compression. Accumulating evidences have shown that exosomes play an irreplaceable role in pre-metastatic niche formation as a communication messenger. However, the function of secreted by breast cells remains incompletely understood metastasis cancer. Methods: Mouse xenograft models intravenous injection were applied for analyzing cell-derived vivo....
microRNA is necessary for osteoclast differentiation, function and survival. It has been reported that miR-199/214 cluster plays important roles in vertebrate skeletal development miR-214 inhibits osteoblast by targeting ATF4. Here, we show up-regulated during osteoclastogenesis from bone marrow monocytes (BMMs) with macrophage colony stimulating factor (M-CSF) receptor activator of nuclear factor-κB ligand (RANKL) induction, which indicates a critical role differentiation. Overexpression...
Abstract Recent studies indicate important roles for long noncoding RNAs (lncRNAs) as essential regulators of myogenesis and adult skeletal muscle regeneration. However, the specific lncRNAs in myogenic differentiation stem cells are still largely unknown. Here we identify a lncRNA that is specifically enriched (myogenesis-associated lncRNA, short, lnc-mg). In mice, conditional knockout lnc-mg results atrophy loss muscular endurance during exercise. Alternatively, muscle-specific...
Mechanical stimulation plays an important role in bone remodeling. Exercise-induced mechanical loading enhances strength, whereas unloading leads to loss. Increasing evidence has demonstrated that long noncoding RNAs (lncRNAs) play key roles diverse biological, physiological and pathological contexts. However, the of lncRNAs mechanotransduction their relationships with formation remain unknown. In this study, we screened mechanosensing osteoblasts identified Neat1, most clearly decreased...
Sustained cardiac pressure overload-induced hypertrophy and pathological remodeling frequently leads to heart failure. Casein kinase-2 interacting protein-1 (CKIP-1) has been identified be an important regulator of cell proliferation, differentiation, apoptosis. However, the physiological role CKIP-1 in is unknown.The results echocardiography histology demonstrate that CKIP-1-deficient mice exhibit spontaneous with aging hypersensitivity hypertrophy, as well. Transgenic cardiac-specific...
Abstract The purpose of this study was to find the circulating microRNAs (miRNAs) co-related with severity coronary artery calcification (CAC) and testify whether selected miRNAs could reflect obstructive disease in symptomatic patients. Patients chest pain moderated risk for (CAD) were characterized calcium score (CACS) from cardiac computed tomography (CT). We analyzed plasma miRNA levels clinical matched 11 CAC (CACS > 100) 6 non-CAC = 0) subjects by microarray profile. Microarray...
Transmembrane and coiled-coil domains 1 (TMCO1) is a recently identified Ca2+ leak channel in the endoplasmic reticulum. TMCO1 dysfunction humans associated with dysmorphism, mental retardation, glaucoma occurrence of cancer. Here we show an essential role osteogenesis mediated by local Ca2+/CaMKII signaling osteoblasts. levels were significantly decreased bone from both osteoporosis patients bone-loss mouse models. Tmco1-/- mice exhibited loss mass altered microarchitecture characteristic...
Background: Without adequate treatment, pathological cardiac hypertrophy induced by sustained pressure overload eventually leads to heart failure. WWP1 (WW domain–containing E3 ubiquitin protein ligase 1) is an important regulator of aging-related pathologies, including cancer and cardiovascular diseases. However, the role in overload–induced remodeling failure yet be determined. Methods: To examine correlation with hypertrophy, we analyzed expression patients mice subjected transverse...
Abstract Osteoclast over-activation leads to bone loss and chloride homeostasis is fundamental importance for osteoclast function. The calcium-activated channel Anoctamin 1 (also known as TMEM16A) an important involved in many physiological processes. However, its role remains unresolved. Here, we identified the existence of show that expression positively correlates with activity. Osteoclast-specific knockout mice exhibit increased mass decreased resorption. Mechanistically, deletion...
Increasing evidence indicates the occurrence of cognitive impairment in astronauts under spaceflight compound conditions, but underlying mechanisms and countermeasures need to be explored. In this study, we found that learning memory abilities were significantly reduced rats a simulated long-duration environment (SLSE), which includes microgravity, isolation confinement, noises, altered circadian rhythms. Dammarane sapogenins (DS), alkaline hydrolyzed products ginsenosides, can enhance...
Interaction between humans and the gut microbiota is important for human physiology. Here, was analyzed via metagenomic sequencing, fluctuations in under conditions of spaceflight were characterized. The composition function substantially affected by spaceflight; however, individual specificity uncompromised. We further confirmed species functional genes from both missions. Resistance virulence spaceflight, but attributions remained stable. Spaceflight markedly microbiota, implying that...
Abstract Microgravity‐induced cardiac remodeling and dysfunction present significant challenges to long‐term spaceflight, highlighting the urgent need elucidate underlying molecular mechanisms develop precise countermeasures. Previous studies have outlined important role of miRNAs in cardiovascular disease progression, with miR‐199a‐3p playing a crucial myocardial injury repair maintenance function. However, specific expression pattern microgravity‐induced remain unclear. We separately...
Abstract Objectives Cardiac Ca 2+ signalling plays an essential role in regulating excitation‐contraction coupling and cardiac remodelling. However, the response of cardiomyocytes to simulated microgravity hypergravity effects on remain unknown. Here, we elucidate mechanisms underlying proliferation remodelling HL‐1 subjected rotation‐simulated 4G hypergravity. Materials Methods The cardiomyocyte cell line was used this study. A clinostat centrifuge were study hypergravity, respectively,...
Osteonecrosis of the femoral head, an intractable but common disease that eventually triggers collapse is characterized by increased osteoclast activity and markedly decreased osteoblast in necrotic region head. MicroRNA (miRNA)-214 (miR-214) may play important roles vertebrate skeletal development inhibiting function targeting activating transcription factor 4 (ATF4) promoting via phosphatase tensin homolog (PTEN). This study revealed significantly levels miR-214 regions, with commensurate...
Abstract Mechanical force loading is essential for maintaining bone homeostasis, and unloading exposure can lead to loss. Osteoclasts are the only resorbing cells play a crucial role in remodeling. The molecular mechanisms underlying mechanical stimulation-induced changes osteoclast function remain be fully elucidated. Our previous research found Ca 2+ -activated Cl − channel Anoctamin 1 (Ano1) was an regulator function. Here, we report that Ano1 mediates responses stimulation. In vitro,...
Bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation and osteoblast function play critical roles in bone formation, which is a highly regulated process. Long noncoding RNAs (lncRNAs) perform diverse functions variety of biological processes, including BMSC differentiation. Although several studies have reported that HOX transcript antisense RNA (HOTAIR) involved differentiation, its effect on formation vivo remains unclear. Here, by constructing transgenic mice with...
Physiological adaptations to microgravity involve alterations in cardiovascular systems. These result cardiac remodeling and orthostatic hypotension. However, the response of left ventricle (LV) right (RV) following hindlimb unloading (HU) reloading (HR) is not clear underlying mechanism remains be understood. In this study, three groups mice were subjected HU by tail suspension for 28 days. Following this, two allowed recover 7 or 14 The control group was treated equally, with exception...
Abstract The CD44 is cellular surface adhesion molecule that involved in physiological processes such as hematopoiesis, lymphocyte homing and limb development. It plays an important role a variety of functions including adhesion, migration, invasion survival. In bone tissue, widely expressed osteoblasts, osteoclasts osteocytes. However, the mechanisms underlying its metabolism remain unclear. We found expression was upregulated during osteoclastogenesis. deficiency vitro significantly...
Physiological adaptations to microgravity involve alterations in cardiovascular, neuromuscular, and neuroendocrine systems. These result cardiac remodeling orthostatic hypotension. At present, the mechanism of induced by remains be disclosed. Casein kinase-2 interacting protein-1 (CKIP-1) is an inhibitor pressure-overload down-regulation phosphorylation HDAC4. However, role CKIP-1 unknown. The purpose this study was determine whether also involved regulation microgravity. We first detected...