A5080761753- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Sperm and Testicular Function
- Spaceflight effects on biology
- Reproductive Biology and Fertility
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Acupuncture Treatment Research Studies
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Selenium in Biological Systems
- Complementary and Alternative Medicine Studies
- Maternal and fetal healthcare
- Peptidase Inhibition and Analysis
- Ginseng Biological Effects and Applications
- Uterine Myomas and Treatments
- Ovarian function and disorders
- Renal and related cancers
- Erythrocyte Function and Pathophysiology
- Cancer Research and Treatments
- Genetics, Aging, and Longevity in Model Organisms
- Glutathione Transferases and Polymorphisms
- Medicinal Plants and Bioactive Compounds
- Apelin-related biomedical research
- Cancer, Hypoxia, and Metabolism
Guangzhou Medical University
2012-2024
First Affiliated Hospital of Guangzhou Medical University
2018-2024
China Astronaut Research and Training Center
2018-2022
Third Affiliated Hospital of Guangzhou Medical University
2010-2020
Mechanical load of the skeleton system is essential for development, growth, and maintenance bone. However, molecular mechanism by which mechanical stimuli are converted into osteogenesis bone formation remains unclear. Here we report that Piezo1, a bona fide mechanotransducer critical various biological processes, plays role in formation. Knockout Piezo1 osteoblast lineage cells disrupts osteoblasts severely impairs structure strength. Bone loss induced unloading blunted knockout mice....
Rationale: Breast cancer preferentially develops osteolytic bone metastasis, which makes patients suffer from pain, fractures and spinal cord compression. Accumulating evidences have shown that exosomes play an irreplaceable role in pre-metastatic niche formation as a communication messenger. However, the function of secreted by breast cells remains incompletely understood metastasis cancer. Methods: Mouse xenograft models intravenous injection were applied for analyzing cell-derived vivo....
Background: Without adequate treatment, pathological cardiac hypertrophy induced by sustained pressure overload eventually leads to heart failure. WWP1 (WW domain–containing E3 ubiquitin protein ligase 1) is an important regulator of aging-related pathologies, including cancer and cardiovascular diseases. However, the role in overload–induced remodeling failure yet be determined. Methods: To examine correlation with hypertrophy, we analyzed expression patients mice subjected transverse...
Mechanical stimulation plays an important role in bone remodeling. Exercise-induced mechanical loading enhances strength, whereas unloading leads to loss. Increasing evidence has demonstrated that long noncoding RNAs (lncRNAs) play key roles diverse biological, physiological and pathological contexts. However, the of lncRNAs mechanotransduction their relationships with formation remain unknown. In this study, we screened mechanosensing osteoblasts identified Neat1, most clearly decreased...
Antioxidant genes and enzymes play important roles in human spermatogenesis. Although low levels of antioxidant enzyme expression are associated with poor sperm quality, it is not clear whether mRNA lower these men than normozoospermic men. In this study, 55 asthenozoospermic oligoasthenozoospermic patients 65 controls were recruited. Quantitative real-time reverse transcription PCR was performed the abundance four known to be spermatogenesis evaluated. These nuclear factor erythroid...
Human-induced pluripotent stem cells (iPSCs) are derived from differentiated somatic using defined factors and provide a renewable source of autologous for cell therapy. Many reprogramming methods have been employed to generate human iPSCs, including the use integrating vectors non-integrating vectors. Maintenance genomic integrity iPSCs is highly desirable if be used in clinical applications. Here, Affymetrix Cytoscan HD array, we investigated aberration profiles 19 lines: 5 embryonic (ESC)...
Background . Diverticulum, one of the long-term sequelae cesarean section, can cause abnormal uterine bleeding and increase risk scar rupture. In this study, we aimed to evaluate efficacy combined laparoscopic hysteroscopic repair, a newly occurring method, treating post-cesarean section diverticulum. Methods Data relating 40 patients with diverticulum who underwent repair were retrospectively analyzed. Preoperative clinical manifestations, size defects, thickness lower segment (LUS),...
Abstract Objectives Cardiac Ca 2+ signalling plays an essential role in regulating excitation‐contraction coupling and cardiac remodelling. However, the response of cardiomyocytes to simulated microgravity hypergravity effects on remain unknown. Here, we elucidate mechanisms underlying proliferation remodelling HL‐1 subjected rotation‐simulated 4G hypergravity. Materials Methods The cardiomyocyte cell line was used this study. A clinostat centrifuge were study hypergravity, respectively,...
The aim of this study is to investigate the relationships among reactive oxygen species (ROS) elevation, histone transition, and seminal cytokine concentrations.Total levels ROS in semen samples from 6560 men were measured. From sample, 118 cases with high 106 controls recruited. Basic parameters histone-to-protamine ratios analyzed, 400 receptor alterations assayed by protein chip, finally 18 cytokines validated each sample using a Bio-Plex Cytokine assay.The results showed that...
Abstract Background Epigenetic modulation by noncoding RNAs substantially contributes to human cancer development, but involvement in bladder remains poorly understood. This study investigated the role of long RNA (lncRNA) lnc-STYK1-2 tumorigenesis cancerous cells. Methods Differential lncRNA and mRNA profiles were characterized high-throughput sequencing combined with validation via quantitative PCR. Bladder cell proliferation was assessed through MTS, migration invasion a Transwell system....
Previous studies have shown an attenuating effect of ginsenoside Re on myocardial injury induced by hypoxia/reoxygenation (H/R). However, the underlying mechanism remains unclear. This study was designed to determine which protects from H/R. HL-1 cells derived AT-1 mouse atrial cardiomyocyte tumor line were divided into control, H/R, and H/R + groups. Cell viability measured CCK-8 assay. ATP levels quantified enzymatic assays. Signaling pathway predicted network pharmacology analyses...
Abstract Aims 3′ untranslated region (3′ UTR) of mRNA is more conserved than other non-coding sequences in vertebrate genomes, and its sequence space has substantially expanded during the evolution higher organisms, which substantiates their significance biological regulation. However, independent role UTR cardiovascular disease was largely unknown. Methods results Using bioinformatics, RNA fluorescent situ hybridization quantitative real-time polymerase chain reaction, we found that coding...
Vascular remodeling is a prominent trait during the development of hypertension, attributable to phenotypic transition vascular smooth muscle cells (VSMCs). Increasing studies demonstrate that microRNA plays an important role in this process. Here, we surprisingly found cell-specific miR-214 knockout (miR-214 cKO) significantly alleviates angiotensin II (Ang II)-induced which has same effect as global mice response Ang stimulation. Under treatment II, cKO exhibit substantially reduced...
Abstract Background Traditional Chinese medicine (TCM) usually involves syndrome differentiation and treatment. Acupuncture, one form of TCM, requires the selection appropriate acupoints needling techniques, but many clinical trials on acupuncture have used fixed protocols without accounting for individual patient differences. We designed a multicenter randomized controlled trial (RCT) to evaluate whether personalized or increases likelihood live births in infertile women with polycystic...
Nasopharyngeal carcinoma (NPC), is one of the most common malignant tumor in southern China and southeast Asia. MYH10 a coding gene NMMHC-IIB protein. Previous studies have shown that expression was up-regulated breast cancer, glioma meningioma. Moreover, it targeted by miR200 family. However, no relevant been found NPC. In present study, we 48 NPC specimens, level lower cancer areas than adjacent normal tissue. depletion can promote migration invasion addition, demonstrated miR-200a has...
Abstract The study aimed to investigate the expression and function of bladder cancer (BC) long noncoding RNAs (lncRNAs) using a high‐throughput platform. High‐throughput sequencing was used compare profiles lncRNA in BC adjacent normal tissues. Quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR), situ hybridization, gene ontology, Kyoto Encyclopedia Genes Genomes analysis were performed verify differential lncRNA. effect overexpression on cellular proliferation,...
Endoplasmic reticulum stress (ERS) has sizeable affect on cancer proliferation, metastasis, immunotherapy and chemoradiotherapy resistance. However, the effect of ERS biochemical recurrence (BCR) prostate patients remains elusive. Here, we generated an ERS-related genes risk signature to evaluate physiological function in PCa with BCR.We collected from GeneCards. The edgeR package was used screen differential TCGA datasets. gene then established using LASSO multivariate Cox regression models...