A5101972952- Spaceflight effects on biology
- MicroRNA in disease regulation
- Bone Metabolism and Diseases
- Circular RNAs in diseases
- Cardiovascular Function and Risk Factors
- Ion channel regulation and function
- Cancer-related molecular mechanisms research
- Pancreatic and Hepatic Oncology Research
- Extracellular vesicles in disease
- Ginseng Biological Effects and Applications
- Cholinesterase and Neurodegenerative Diseases
- Erythrocyte Function and Pathophysiology
- RNA Research and Splicing
- RNA modifications and cancer
- Medicinal Plants and Neuroprotection
- Genetics and Neurodevelopmental Disorders
- Peptidase Inhibition and Analysis
- Genetics, Aging, and Longevity in Model Organisms
- Hematopoietic Stem Cell Transplantation
- Diet and metabolism studies
- Bone health and treatments
- Gallbladder and Bile Duct Disorders
- Cardiac Fibrosis and Remodeling
- Anesthesia and Neurotoxicity Research
- High Altitude and Hypoxia
China Astronaut Research and Training Center
2015-2024
Guangxi Medical University
2022-2024
Guangdong Medical College
2022-2024
Air Force Medical University
2021-2023
State Key Laboratory of Space Medicine Fundamentals and Application
2017
Mechanical load of the skeleton system is essential for development, growth, and maintenance bone. However, molecular mechanism by which mechanical stimuli are converted into osteogenesis bone formation remains unclear. Here we report that Piezo1, a bona fide mechanotransducer critical various biological processes, plays role in formation. Knockout Piezo1 osteoblast lineage cells disrupts osteoblasts severely impairs structure strength. Bone loss induced unloading blunted knockout mice....
MicroRNAs have an important role in bone homeostasis. However, the detailed mechanism of microRNA-mediated intercellular communication between cells remains elusive. Here, we report that osteoclasts secrete microRNA-enriched exosomes, by which miR-214 is transferred into osteoblasts to inhibit their function. In a coculture system, inhibition exosome formation and secretion prevented transportation. Exosomes specifically recognized through interaction ephrinA2 EphA2. osteoclast-specific...
Rationale: Breast cancer preferentially develops osteolytic bone metastasis, which makes patients suffer from pain, fractures and spinal cord compression. Accumulating evidences have shown that exosomes play an irreplaceable role in pre-metastatic niche formation as a communication messenger. However, the function of secreted by breast cells remains incompletely understood metastasis cancer. Methods: Mouse xenograft models intravenous injection were applied for analyzing cell-derived vivo....
microRNA is necessary for osteoclast differentiation, function and survival. It has been reported that miR-199/214 cluster plays important roles in vertebrate skeletal development miR-214 inhibits osteoblast by targeting ATF4. Here, we show up-regulated during osteoclastogenesis from bone marrow monocytes (BMMs) with macrophage colony stimulating factor (M-CSF) receptor activator of nuclear factor-κB ligand (RANKL) induction, which indicates a critical role differentiation. Overexpression...
Mechanical stimulation plays an important role in bone remodeling. Exercise-induced mechanical loading enhances strength, whereas unloading leads to loss. Increasing evidence has demonstrated that long noncoding RNAs (lncRNAs) play key roles diverse biological, physiological and pathological contexts. However, the of lncRNAs mechanotransduction their relationships with formation remain unknown. In this study, we screened mechanosensing osteoblasts identified Neat1, most clearly decreased...
Abstract The purpose of this study was to find the circulating microRNAs (miRNAs) co-related with severity coronary artery calcification (CAC) and testify whether selected miRNAs could reflect obstructive disease in symptomatic patients. Patients chest pain moderated risk for (CAD) were characterized calcium score (CACS) from cardiac computed tomography (CT). We analyzed plasma miRNA levels clinical matched 11 CAC (CACS > 100) 6 non-CAC = 0) subjects by microarray profile. Microarray...
Transmembrane and coiled-coil domains 1 (TMCO1) is a recently identified Ca2+ leak channel in the endoplasmic reticulum. TMCO1 dysfunction humans associated with dysmorphism, mental retardation, glaucoma occurrence of cancer. Here we show an essential role osteogenesis mediated by local Ca2+/CaMKII signaling osteoblasts. levels were significantly decreased bone from both osteoporosis patients bone-loss mouse models. Tmco1-/- mice exhibited loss mass altered microarchitecture characteristic...
Background: Without adequate treatment, pathological cardiac hypertrophy induced by sustained pressure overload eventually leads to heart failure. WWP1 (WW domain–containing E3 ubiquitin protein ligase 1) is an important regulator of aging-related pathologies, including cancer and cardiovascular diseases. However, the role in overload–induced remodeling failure yet be determined. Methods: To examine correlation with hypertrophy, we analyzed expression patients mice subjected transverse...
Abstract Osteoclast over-activation leads to bone loss and chloride homeostasis is fundamental importance for osteoclast function. The calcium-activated channel Anoctamin 1 (also known as TMEM16A) an important involved in many physiological processes. However, its role remains unresolved. Here, we identified the existence of show that expression positively correlates with activity. Osteoclast-specific knockout mice exhibit increased mass decreased resorption. Mechanistically, deletion...
Increasing evidence indicates the occurrence of cognitive impairment in astronauts under spaceflight compound conditions, but underlying mechanisms and countermeasures need to be explored. In this study, we found that learning memory abilities were significantly reduced rats a simulated long-duration environment (SLSE), which includes microgravity, isolation confinement, noises, altered circadian rhythms. Dammarane sapogenins (DS), alkaline hydrolyzed products ginsenosides, can enhance...
Abstract Objectives Cardiac Ca 2+ signalling plays an essential role in regulating excitation‐contraction coupling and cardiac remodelling. However, the response of cardiomyocytes to simulated microgravity hypergravity effects on remain unknown. Here, we elucidate mechanisms underlying proliferation remodelling HL‐1 subjected rotation‐simulated 4G hypergravity. Materials Methods The cardiomyocyte cell line was used this study. A clinostat centrifuge were study hypergravity, respectively,...
Abstract Mechanical force loading is essential for maintaining bone homeostasis, and unloading exposure can lead to loss. Osteoclasts are the only resorbing cells play a crucial role in remodeling. The molecular mechanisms underlying mechanical stimulation-induced changes osteoclast function remain be fully elucidated. Our previous research found Ca 2+ -activated Cl − channel Anoctamin 1 (Ano1) was an regulator function. Here, we report that Ano1 mediates responses stimulation. In vitro,...
Bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation and osteoblast function play critical roles in bone formation, which is a highly regulated process. Long noncoding RNAs (lncRNAs) perform diverse functions variety of biological processes, including BMSC differentiation. Although several studies have reported that HOX transcript antisense RNA (HOTAIR) involved differentiation, its effect on formation vivo remains unclear. Here, by constructing transgenic mice with...
Physiological adaptations to microgravity involve alterations in cardiovascular systems. These result cardiac remodeling and orthostatic hypotension. However, the response of left ventricle (LV) right (RV) following hindlimb unloading (HU) reloading (HR) is not clear underlying mechanism remains be understood. In this study, three groups mice were subjected HU by tail suspension for 28 days. Following this, two allowed recover 7 or 14 The control group was treated equally, with exception...
Abstract The CD44 is cellular surface adhesion molecule that involved in physiological processes such as hematopoiesis, lymphocyte homing and limb development. It plays an important role a variety of functions including adhesion, migration, invasion survival. In bone tissue, widely expressed osteoblasts, osteoclasts osteocytes. However, the mechanisms underlying its metabolism remain unclear. We found expression was upregulated during osteoclastogenesis. deficiency vitro significantly...
Physiological adaptations to microgravity involve alterations in cardiovascular, neuromuscular, and neuroendocrine systems. These result cardiac remodeling orthostatic hypotension. At present, the mechanism of induced by remains be disclosed. Casein kinase-2 interacting protein-1 (CKIP-1) is an inhibitor pressure-overload down-regulation phosphorylation HDAC4. However, role CKIP-1 unknown. The purpose this study was determine whether also involved regulation microgravity. We first detected...
Spaceflight leads to health risks including bone demineralization, skeletal muscle atrophy, cardiovascular dysfunction, and disorders of almost all physiologic systems. However, the impacts microgravity on blood lineage cells hematopoietic stem (HSCs) in vivo are largely unknown. In this study, we analyzed peripheral samples from 6 astronauts who had participated spaceflight missions found significant changes several cell populations at different time points. These dynamic alterations role...
Receptor Expression-Enhancing Protein 3 (REEP3) serves as a pivotal enzyme crucial for endoplasmic reticulum (ER) clearance during mitosis and is implicated in the advancement of diverse malignancies. Nonetheless, biological role mechanisms REEP3 pancreatic cancer patients, along with its interplay immune infiltration, remain inadequately elucidated. In this study, we initially analyzed differential expression between tissues normal pancreas using Cancer Genome Atlas (TCGA), GTEx Gene...
Cardiac muscle is extremely sensitive to changes in loading conditions; the microgravity during space flight can cause cardiac remodeling and function decline. At present, mechanism of microgravity-induced remains be revealed. WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) an important activator pressure overload-induced by stabilizing disheveled segment polarity proteins 2 (DVL2) activating calcium-calmodulin-dependent kinase II (CaMKII)/histone deacetylase 4...
Previous studies have shown an attenuating effect of ginsenoside Re on myocardial injury induced by hypoxia/reoxygenation (H/R). However, the underlying mechanism remains unclear. This study was designed to determine which protects from H/R. HL-1 cells derived AT-1 mouse atrial cardiomyocyte tumor line were divided into control, H/R, and H/R + groups. Cell viability measured CCK-8 assay. ATP levels quantified enzymatic assays. Signaling pathway predicted network pharmacology analyses...