A5100431933- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Cancer Research and Treatments
- Protein Degradation and Inhibitors
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Immunotherapy and Immune Responses
- Peptidase Inhibition and Analysis
- Histone Deacetylase Inhibitors Research
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- interferon and immune responses
- RNA Interference and Gene Delivery
- Genetics and Neurodevelopmental Disorders
- Chromosomal and Genetic Variations
- Skin and Cellular Biology Research
- Autophagy in Disease and Therapy
- Nanoplatforms for cancer theranostics
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Cancer Genomics and Diagnostics
- Immune Cell Function and Interaction
- Galectins and Cancer Biology
- Cancer Mechanisms and Therapy
Medical College of Wisconsin
2025
Liaoning Cancer Hospital & Institute
2025
China Medical University
2020-2025
The First Affiliated Hospital, Sun Yat-sen University
2025
Zhongshan Hospital
2025
Fudan University
2023-2025
Sun Yat-sen University
2008-2025
Bioscience (China)
2025
University of Science and Technology of China
2011-2024
Liaocheng People's Hospital
2024
Liver metastasis represents one of the most frequent malignant diseases with no effective treatment. Functional reprogramming Kupffer cells (KCs), largest population hepatic macrophages, holds promise for treating liver cancer, but remains seldom exploited. Taking advantage superior capacity KCs to capture circulating bacteria, we report that a single administration attenuated Escherichia coli producing clustered regularly interspersed short palindromic repeats CasΦ (CRISPR/CasΦ) machinery...
ARH-77 cells do not adhere to type I collagen and readily invade into gels, but following expression of the transmembrane heparan sulfate proteoglycan syndecan-1, they bind fail invade. We now show that transfected with syndecan-2 or syndecan-4 also are non-invasive. In contrast, glycosylphosphatidylinositol-anchored glypican-1 remain invasive, even though glypican- syndecan-expressing have similar surface levels sulfate, their proteoglycans affinities for collagen. Analysis expressing...
Background: Without adequate treatment, pathological cardiac hypertrophy induced by sustained pressure overload eventually leads to heart failure. WWP1 (WW domain–containing E3 ubiquitin protein ligase 1) is an important regulator of aging-related pathologies, including cancer and cardiovascular diseases. However, the role in overload–induced remodeling failure yet be determined. Methods: To examine correlation with hypertrophy, we analyzed expression patients mice subjected transverse...
The NAD+-dependent deacetylase Sirt1 has been implicated in the prevention of many age-related diseases, including cancer, type 2 diabetes, and cardiovascular disease. Resveratrol, a plant polyphenol, exhibits antiaging, antitumor, vascular protection effects by activating Sirt1. However, molecular mechanism activation as induced resveratrol remains unclear. By knockdown/rescue experiments, fluorometric activity assay, immunoprecipitation, pull-down assays, we identify here that tumor...
Abstract Owing to their ability rapidly proliferate in specific niches and amenability genetic manipulation, bacteria are frequently studied as potential diagnostic or therapeutic bioagents a range of pathological contexts. A sustained oxygen supply within solid tumors is essential order achieve positive radiotherapy (RT) outcomes, these intratumoral levels necessary facilitate RT‐induced reactive species (ROS) generation. In this study, genetically engineered variant the tumor‐targeting...
SAD1/UNC84 domain protein-2 (SUN2) plays a tumor suppressor role in various types of cancer by inhibiting cell proliferation, migration and promoting apoptosis. However, the post-translational regulation SUN2 cellular mechanism responsible for its proteasomal degradation remains largely unknown. Here, we show that FBXO2, an E3 ubiquitin ligase F-box proteins (FBPs) family targets glycosylated ubiquitination via ubiquitin-proteasome system (UPS). By integrating Cancer Genome Atlas (TCGA),...
There is evidence that the triazine herbicide atrazine, which used extensively, present in both surface water and groundwater, its interfering effect on immune systems, endocrine tumours has been reported by laboratory epidemiological studies. This study explored how atrazine affected 4T1 breast cancer cell development vitro vivo. The obtained results showed after exposure to proliferation tumour volume were significantly increased expression of MMP2, MMP7, MMP9 was upregulated. thymus...
Multiple myeloma (MM) is an incurable plasma cell malignancy. Although Dexamethasone (Dex) the most widely used therapeutic drug in MM treatment, patients develop Dex resistance leading to progressive disease, demanding urgent need investigate mechanisms driving and new reagents address this problem. We propose SUMOylation as a potential mechanism regulating inhibition can enhance sensitivity MM.
Abstract Hepatocellular carcinoma (HCC) is a common solid tumor with high rate of recurrence and mortality. Anti-angiogenesis drugs have been used for the therapy HCC. However, anti-angiogenic drug resistance commonly occurs during HCC treatment. Thus, identification novel VEGFA regulator would be better understanding progression resistance. Ubiquitin specific protease 22 (USP22) as deubiquitinating enzyme, participates in variety biological processes numerous tumors. While molecular...
Background: The mitotic kinesin, KIF18A, is required for proliferation of cancer cells that exhibit chromosome instability (CIN), implicating it as a promising target treatment subset aggressive tumor types. Determining regions the KIF18A protein to inhibition will be important design and optimization effective small molecule inhibitors. Methods: In this study, we used cultured cell models investigate effects mutating S284 within alpha-4 helix which was previously identified phosphorylated...
The ubiquitin-proteasome system (UPS) is essential for cellular homeostasis, regulating the degradation of proteins involved in key processes such as cell cycle, apoptosis, and DNA repair. Dysregulation UPS implicated hepatocellular carcinoma (HCC), contributing to tumor progression therapeutic resistance. cereblon (CRBN) E3 ubiquitin ligase complex a crucial component UPS, particularly modulating protein response small-molecule modulators like thalidomide. However, endogenous substrates...
It has been shown that guanine-rich DNA can fold into a G-quadruplex with certain metal cations. The spectral characteristics, thermostability, and kinetics for the formation of Pb2+-driven thrombin-binding aptamer (TBA) were measured in current work using combination ultraviolet (UV) circular dichroism (CD) spectroscopy along stopped-flow technique. CD spectra demonstrated TBA could unique strong positive peak at 312 nm. Analysis titration data reveals binding stoichiometry is 1:1 Pb(NO3)2,...
βKlotho is a regulator in multiple metabolic processes, while its role cancer remains unclear. We found the expression of was down-regulated human hepatocellular carcinoma tissues compared with that paired adjacent non-tumourous liver tissues. Hepatoma cells also showed decreased normal hepatocyte cells. Reintroduction into hepatoma inhibited their proliferation. The anti-proliferative effect might be linked G1 to S phase arrest, which mediated by Akt/GSK-3β/cyclin D1 signaling, since forced...
Chromosome segregation in mitosis is orchestrated by the dynamic interactions between kinetochore and spindle microtubules. Our recent study shows that mitotic motor CENP-E cooperates with SKAP to orchestrate an accurate chromosome movement mitosis. However, it remains elusive how core microtubule binding activity KMN (KNL1-MIS12-NDC80) regulates plus-end dynamics. Here, we identify a novel interaction MIS13 orchestrates physically interacts specifies localization of SKAP. Suppression small...
The type III secretion system (T3SS) of Edwardsiella tarda plays an important role in infection by translocating effector proteins into host cells. EseB, a component required for translocation, is reported to mediate autoaggregation E. tarda. In this study, we demonstrate that EseB forms filamentous appendages on the surface and biofilm formation Dulbecco's modified Eagle's medium (DMEM). Biofilm DMEM does not require FlhB, essential assembling flagella. Dynamic analysis filament formation,...
Abstract Heterogeneous nuclear ribonucleoprotein L (hnRNPL) is a type of RNA binding protein that highly expressed in variety tumors and plays vital role tumor progression. However, its post-translational regulation through ubiquitin-mediated proteolysis the cellular mechanism responsible for proteasomal degradation remains unclear. F-box proteins (FBPs) function as substrate recognition subunits SCF ubiquitin ligase complexes directly bind to substrates. The aberrant expression or mutation...
Do variants in helicase for meiosis 1 (HFM1) account male infertility humans?Biallelic HFM1 cause human owing to non-obstructive azoospermia (NOA) with impaired crossover formation and meiotic metaphase I (MMI) arrest.HFM1 encodes an evolutionarily conserved DNA that is essential completion of meiosis. The null mutants Hfm1 or its ortholog multiple organisms displayed spermatogenic arrest at the MMI deficiencies synapsis severe defects formation. Although were found infertile men...