A5101865270- Plant biochemistry and biosynthesis
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- DNA Repair Mechanisms
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Plant Physiology and Cultivation Studies
- Reproductive Biology and Fertility
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Photosynthetic Processes and Mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Postharvest Quality and Shelf Life Management
- Genetic Mapping and Diversity in Plants and Animals
- Plant Stress Responses and Tolerance
- Lipid metabolism and biosynthesis
- Carcinogens and Genotoxicity Assessment
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- Adipose Tissue and Metabolism
- Genetics, Aging, and Longevity in Model Organisms
University of Science and Technology of China
2014-2025
Harvard University
2021-2025
Guizhou University
2023-2025
Nanjing Agricultural University
2012-2024
Massachusetts General Hospital
2020-2024
Hefei National Center for Physical Sciences at Nanoscale
2014-2023
Zhongnan Hospital of Wuhan University
2023
Wuhan University
2023
Shanghai University of Traditional Chinese Medicine
2023
Center for Excellence in Molecular Cell Science
2018-2022
The draft genome of the pear ( Pyrus bretschneideri ) using a combination BAC-by-BAC and next-generation sequencing is reported. A 512.0-Mb sequence corresponding to 97.1% estimated size this highly heterozygous species assembled with 194× coverage. High-density genetic maps comprising 2005 SNP markers anchored 75.5% all 17 chromosomes. encodes 42,812 protein-coding genes, these, ∼28.5% encode multiple isoforms. Repetitive sequences 271.9 Mb in length, accounting for 53.1% genome, are...
Pear (Pyrus) is a globally grown fruit, with thousands of cultivars in five domesticated species and dozens wild species. However, little known about the evolutionary history these pear what has contributed to distinct phenotypic traits between Asian pears European pears.
Heat shock transcription factors (Hsfs), which act as important transcriptional regulatory proteins in eukaryotes, play a central role controlling the expression of heat-responsive genes. At present, genomes Chinese white pear (‘Dangshansuli’) and five other Rosaceae fruit crops have been fully sequenced. However, information about Hsfs gene family these species is limited, evolutionary history also remains unresolved. In this study, 137 Hsf genes were identified from six (Pyrus...
Asthenozoospermia is a common cause of male infertility, but its etiology remains incompletely understood. We recruited three Pakistani infertile brothers, born to first-cousin parents, displaying idiopathic asthenozoospermia no ciliary-related symptoms. Whole-exome sequencing identified missense variant (c.G5408A, p.C1803Y) in DNAH17, functionally uncharacterized gene, recessively cosegregating with the family. specifically expressed testes, was localized sperm flagella, and mutation did...
Pear is an important fruit crop of the Rosaceae family and has experienced two rounds ancient whole-genome duplications (WGDs). However, whether different types gene evolved differently after duplication remains unclear in pear genome. In this study, we identified modes pear. Duplicate genes derived from WGD, tandem, proximal, retrotransposed, DNA-based transposed or dispersed differ genomic distribution, features, selection pressure, expression divergence, regulatory divergence biological...
Fanconi anemia (FA) genes play important roles in spermatogenesis. In mice, disruption of Fancm impairs male fertility and testicular integrity, but whether FANCM pathogenic variants (PV) similarly affect men is unknown. Here we characterize a Pakistani family having three infertile brothers, two manifesting oligoasthenospermia one exhibiting azoospermia, born to first-cousin parents. A homozygous PV (c.1946_1958del, p.P648Lfs*16) was found cosegregating with infertility. Our objective...
Meiotic recombination permits exchange of genetic material between homologous chromosomes. The replication protein A (RPA) complex, the predominant ssDNA-binding is required for nearly all aspects DNA metabolism, but its role in mammalian meiotic remains unknown due to embryonic lethality RPA mutant mice. a heterotrimer RPA1, RPA2, and RPA3. We find that loss largest subunit, leads disappearance RPA2 RPA3, resulting absence complex. Using an inducible germline-specific inactivation strategy,...
Adipose tissue browning and systemic energy expenditure provide a defense mechanism against obesity associated metabolic diseases. In high-cholesterol Western diet-fed mice, mast cell (MC) inactivation ameliorates insulin resistance improves the rate, but direct role of adipose MCs in thermogenesis remains unproven. Here, we report that adrenoceptor agonist norepinephrine-stimulated rate subcutaneous (SAT) are enhanced MC-deficient Kitw-sh/w-sh mice MC-stabilized wild-type on chow diet. MC...
Abstract Premature ovarian insufficiency (POI) is a major cause of reduced female fertility and affects approximately 1% women under 40 years age. Recent advances emphasize the genetic heterogeneity POI. Fanconi anemia (FA) genes, traditionally known for their essential roles in DNA repair cytogenetic instability, have been demonstrated to be involved meiosis germ cell development. Here, we conducted whole-exome sequencing (WES) 50 Han Chinese patients with Rare missense variants were...
The DSB machinery, which induces the programmed DNA double-strand breaks (DSBs) in leptotene and zygotene stages during meiosis, is suppressed before onset of pachytene stage. However, biological significance underlying mechanisms remain largely unclear. Here, we report that ZFP541 indispensable for suppression formation after mid-pachytene. deletion Zfp541 mice causes aberrant recruitment machinery to chromosome axes generation massive DSBs late diplotene spermatocytes, leading meiotic...
RAD51AP2 is required specifically for efficient meiotic recombination to form crossover between X and Y chromosomes.
Orphan genes play crucial roles in diverse biological processes, but the evolutionary trajectories and functional divergence remain largely unexplored. The Theaceae family, including economically culturally important tea plant, offers a distinctive model to examine these aspects. Here, we integrated Nanopore long-read sequencing, Illumina short-read Hi-C methods decode pseudo-chromosomal genome assembly of Stewartia sinensis, from earliest-diverging tribe Theaceae, spanning 2.95 Gb....
Abstract During meiosis, at least one crossover is selectively generated per pair of homologous chromosomes through recombination to ensure their faithful segregation. The molecular mechanisms controlling meiotic recombination, particularly in XY that share a tiny region homology (i.e., the pseudoautosomal region, PAR), remain poorly understood. Here, we identify S100PBP as key modulator both and autosomal mice. S100pbp- knockout mice exhibit male infertility spermatogenesis arrest metaphase...
Abstract Background The perennial, Oryza rufipogon distributed from Asia to Australia and the annual O. meridionalis indigenous are AA genome species in . However, recent research has demonstrated that Australian perennial populations have maternal genomes more closely related those of than found Asian suggesting perennials may represent a new distinct gene pool for rice. Results Analysis an core collection covering Oceania revealed some had organellar O (meridionalis-type). accessions New...
There are more than 2300 genes that predominantly expressed in mouse testes. The role of hundreds these has been studied spermatogenesis but still there many whose function is unknown. Gene knockout (KO) strategy mice widely used for vivo study gene function. present was designed to explore the four genes: Tex37, Ccdc73, Prss55 and Nxt2, which were evolutionarily conserved eutherians. We found had a testis-enriched expression pattern except Nxt2. knocked out by CRISPR/Cas9 individually all...
Do variants in helicase for meiosis 1 (HFM1) account male infertility humans?Biallelic HFM1 cause human owing to non-obstructive azoospermia (NOA) with impaired crossover formation and meiotic metaphase I (MMI) arrest.HFM1 encodes an evolutionarily conserved DNA that is essential completion of meiosis. The null mutants Hfm1 or its ortholog multiple organisms displayed spermatogenic arrest at the MMI deficiencies synapsis severe defects formation. Although were found infertile men...
Pear (Pyrus ssp.) belongs to Rosaceae and is an important fruit tree widely cultivated around the world. Currently, challenges cope with burgeoning sets of multiomics data are rapidly increasing. Here, we constructed Multiomics Database (PearMODB) by integrating genome, transcriptome, epigenome population variation data, aimed provide a portal for accessing analyzing pear data. A variety online tools were built including gene search, BLAST, JBrowse, expression heatmap, synteny analysis...