A5060557259- Plant Physiology and Cultivation Studies
- Plant Pathogens and Fungal Diseases
- Horticultural and Viticultural Research
- Plant Reproductive Biology
- Postharvest Quality and Shelf Life Management
- Plant Gene Expression Analysis
- Berry genetics and cultivation research
- Plant-Microbe Interactions and Immunity
- Genetic diversity and population structure
- Plant biochemistry and biosynthesis
- Fungal Plant Pathogen Control
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Yeasts and Rust Fungi Studies
- Plant pathogens and resistance mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Genomics and Phylogenetic Studies
- Plant and animal studies
- Agriculture, Soil, Plant Science
- Plant Virus Research Studies
- Plant Molecular Biology Research
- Botanical Studies and Applications
- Plant Pathogens and Resistance
- Chromosomal and Genetic Variations
- Insect-Plant Interactions and Control
Plant & Food Research
2016-2025
Palmerston North Hospital
2010-2022
University of Otago
2018-2021
AgResearch
2007-2021
Genomics (United Kingdom)
2020-2021
Statistics New Zealand
2015
Planta
2014
Fondazione Edmund Mach
2012-2014
Hawke's Bay Regional Council
2012
University of Auckland
2012
Mutations in the genes encoding for either biosynthetic or transcriptional regulation of anthocyanin pathway have been linked to color phenotypes. Generally, this is a loss function resulting reduction change distribution anthocyanin. Here, we describe rearrangement upstream regulatory region gene an apple (Malus x domestica) anthocyanin-regulating transcription factor, MYB10. We show that modification responsible increasing level throughout plant produce striking phenotype includes red...
The biosynthesis of anthocyanin in many plants is affected by environmental conditions. In apple (Malus × domestica Borkh.), concentrations fruit anthocyanins are lower under hot climatic We examined the accumulation peel maturing 'Mondial Gala' and 'Royal apples, grown both temperate climates, using artificial heating on-tree fruit. Heat caused a dramatic reduction concentration transcripts genes biosynthetic pathway. Heating rapidly reduced expression R2R3 MYB transcription factor (MYB10)...
Abstract Anthocyanin accumulation is coordinated in plants by a number of conserved transcription factors. In apple (Malus × domestica), an R2R3 MYB factor has been shown to control fruit flesh and foliage anthocyanin pigmentation (MYB10) skin color (MYB1). However, the pattern expression allelic variation at these loci does not explain all anthocyanin-related phenotypes. One such example open-pollinated seedling cv Sangrado that green develops red cortex late maturity. We used methods...
As high-throughput genetic marker screening systems are essential for a range of genetics studies and plant breeding applications, the International RosBREED SNP Consortium (IRSC) has utilized Illumina Infinium® II system to develop medium- tool genome-wide evaluation allelic variation in apple (Malus×domestica) germplasm. For discovery, 27 cultivars were chosen represent worldwide germplasm re-sequenced at low coverage with Genome Analyzer II. Following alignment these sequences whole...
We present a draft assembly of the genome European pear (Pyrus communis) 'Bartlett'. Our was developed employing second generation sequencing technology (Roche 454), from single-end, 2 kb, and 7 kb insert paired-end reads using Newbler (version 2.7). It contains 142,083 scaffolds greater than 499 bases (maximum scaffold length 1.2 Mb) covers total 577.3 Mb, representing most expected 600 Mb Pyrus genome. A 829,823 putative single nucleotide polymorphisms (SNPs) were detected re-sequencing...
High-density SNP arrays for genome-wide assessment of allelic variation have made high resolution genetic characterization crop germplasm feasible. A medium density array apple, the IRSC 8 K array, has been successfully developed and used screens bi-parental populations. However, number robust well-distributed markers contained on this was not sufficient to perform association analyses in wider sets, or Pedigree-Based Analysis at precision, because rapid decay linkage disequilibrium. We...
The genome sequence of apple (Malus×domestica Borkh.) was published more than a year ago, which helped develop an 8K SNP chip to assist in implementing genomic selection (GS). In breeding programmes, GS can be used obtain values (GEBV) for choosing next-generation parents or selections further testing as potential commercial cultivars at very early stage. Thus has the accelerate efficiency significantly because decreased generation interval increased intensity. We evaluated accuracy...
Pear (Pyrus) is a globally grown fruit, with thousands of cultivars in five domesticated species and dozens wild species. However, little known about the evolutionary history these pear what has contributed to distinct phenotypic traits between Asian pears European pears.
Most published genome sequences are drafts, and most dominated by computational gene prediction. Draft genomes typically incorporate considerable sequence data that not assigned to chromosomes, predicted genes without quality confidence measures. The current Actinidia chinensis (kiwifruit) 'Hongyang' draft has 164 Mb of unassigned pseudo-chromosomes, omissions have been identified in the models.A second an A. (genotype Red5) was fully sequenced. This new resulted a 554.0 assembly with all...
Integrating plant genomics and classical breeding is a challenge for both breeders molecular biologists. Marker-assisted selection (MAS) tool that can be used to accelerate the development of novel apple varieties such as cultivars have fruit with anthocyanin through core. In addition, determining inheritance alleles, one responsible red flesh, adds our understanding allelic variation. Our goal was map candidate biosynthetic regulatory genes in population segregating flesh phenotypes. We...
Abstract Background Kiwifruit (Actinidia spp.) are a relatively new, but economically important crop grown in many different parts of the world. Commercial success is driven by development new cultivars with novel consumer traits including flavor, appearance, healthful components and convenience. To increase our understanding genetic diversity gene-based control these key Actinidia, we have produced collection 132,577 expressed sequence tags (ESTs). Results The ESTs were derived mainly from...
Abstract Background Auxin is an important phytohormone for fleshy fruit development, having been shown to be involved in the initial signal fertilisation, size through control of cell division and expansion, ripening related events. There considerable knowledge auxin-related genes, mostly from work model species. With apple genome now available, it possible carry out genomics studies on genes identify that may play roles specific stages development. Results High amounts auxin seed compared...
Although flowering in mature fruit trees is recurrent, floral induction can be strongly inhibited by concurrent fruiting, leading to a pattern of irregular fruiting across consecutive years referred as biennial bearing. The genetic determinants bearing apple were investigated using the 114 individuals from an F(1) population 122 genotypes, 'Starkrimson' (strong bearer)×'Granny Smith' (regular bearer) cross. number inflorescences, and mass harvested recorded over 6 used calculate 26 variables...
Understanding the genetic architecture of quantitative traits is important for developing genome-based crop improvement methods. Genome-wide association study (GWAS) a powerful technique mining novel functional variants. Using family-based design involving 1,200 apple (Malus × domestica Borkh.) seedlings genotyped an 8K SNP array, we report first systematic evaluation relative contributions different genomic regions to various related eating quality and susceptibility some physiological...
The polyphenolic products of the phenylpropanoid pathway, including proanthocyanidins, anthocyanins and flavonols, possess antioxidant properties that may provide health benefits. To investigate genetic architecture control their biosynthesis in apple fruit, various compounds were quantified progeny from a 'Royal Gala' × 'Braeburn' population segregating for content, using ultra high performance liquid chromatography extracts derived fruit cortex skin. Construction maps enabled detection 79...
Abstract Background The fragmented nature of most draft plant genomes has hindered downstream gene discovery, trait mapping for breeding, and other functional genomics applications. There is a pressing need to improve or finish genome assemblies. Findings Here, we present chromosome-scale assembly the black raspberry using single-molecule real-time Pacific Biosciences sequencing high-throughput chromatin conformation capture (Hi-C) scaffolding. updated V3 contig N50 5.1 Mb, representing an...
The molecular genetic mechanisms underlying fruit size remain poorly understood in perennial crops, despite being an important agronomic trait. Here we show that the expression level of a microRNA gene (miRNA172) influences apple. A transposon insertional allele miRNA172 showing reduced associates with large apple breeding population, whereas over-expression transgenic significantly reduces size. was found to be co-located major quantitative trait locus, fixed cultivated apples and their...