A5022979968- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Plant pathogens and resistance mechanisms
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Transgenic Plants and Applications
- Phytochemicals and Antioxidant Activities
- Horticultural and Viticultural Research
- Plant Molecular Biology Research
- Plant Pathogens and Fungal Diseases
- Plant Physiology and Cultivation Studies
- Fungal and yeast genetics research
- Plant Parasitism and Resistance
- Plant Virus Research Studies
- Plant Reproductive Biology
- Natural product bioactivities and synthesis
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Antioxidant Activity and Oxidative Stress
- Light effects on plants
- Plant tissue culture and regeneration
- Fermentation and Sensory Analysis
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- Microbial Natural Products and Biosynthesis
Plant & Food Research
2016-2025
Fondazione Edmund Mach
2012
Hawke's Bay Regional Council
2012
University of Auckland
2012
Centre de Génétique Moléculaire
2002
Centre National de la Recherche Scientifique
2000-2002
Plants require sophisticated regulatory mechanisms to ensure the degree of anthocyanin pigmentation is appropriate myriad developmental and environmental signals. Central this process are activity MYB-bHLH-WD repeat (MBW) complexes that regulate transcription genes. In study, gene network regulates synthesis in petunia (Petunia hybrida) has been characterized. Genetic molecular evidence show R2R3-MYB, MYB27, an repressor functions as part MBW complex represses through its C-terminal EAR...
Mutations in the genes encoding for either biosynthetic or transcriptional regulation of anthocyanin pathway have been linked to color phenotypes. Generally, this is a loss function resulting reduction change distribution anthocyanin. Here, we describe rearrangement upstream regulatory region gene an apple (Malus x domestica) anthocyanin-regulating transcription factor, MYB10. We show that modification responsible increasing level throughout plant produce striking phenotype includes red...
Abstract Anthocyanin accumulation is coordinated in plants by a number of conserved transcription factors. In apple (Malus × domestica), an R2R3 MYB factor has been shown to control fruit flesh and foliage anthocyanin pigmentation (MYB10) skin color (MYB1). However, the pattern expression allelic variation at these loci does not explain all anthocyanin-related phenotypes. One such example open-pollinated seedling cv Sangrado that green develops red cortex late maturity. We used methods...
Summary Variation in anthocyanin biosynthesis pear fruit provides genetic germplasm resources for breeding, while dwarfing is an important agronomic trait, which beneficial to reduce the management costs and allow implementation of high‐density cultivation. Here, we combined bulked segregant analysis (BSA), quantitative trait loci (QTL), structural variation (SV) identify a 14‐bp deletion caused frame shift mutation resulted premature translation termination B‐box (BBX) family zinc...
The anthocyanin biosynthetic pathway is regulated by a transcription factor complex consisting of an R2R3 MYB, bHLH, and WD40. Although MYBs belonging to the anthocyanin-activating class have been identified in many plants, their role well elucidated, subgroups bHLH implicated regulation seem be more complex. It not clear whether these potential partners are biologically interchangeable with redundant functions, or even if heterodimers involved. In this study, AcMYB110, MYB isolated from...
Summary Consumers of whole foods, such as fruits, demand consistent high quality and seek varieties with enhanced health properties, convenience or novel taste. We have raised the polyphenolic content apple by genetic engineering anthocyanin pathway using transcription factor MYB 10 . These apples very concentrations foliar, flower fruit anthocyanins, especially in peel. Independent lines were examined for impacts on tree growth, photosynthesis characteristics. Fruit analysed changes...
Summary Apple ( Malus × domestica ) accumulates bioactive ursane‐, oleanane‐, and lupane‐type triterpenes in its fruit cuticle, but their biosynthetic pathway is still poorly understood. We used a homology‐based approach to identify functionally characterize two new oxidosqualene cyclases (Md OSC 4 Md 5) one cytochrome P450 CYP 716A175). The gene expression patterns of these enzymes previously described were further studied 20 apple cultivars with contrasting triterpene profiles. encodes...
A mutant allele of the transcription factor gene MYB10 from apple induces anthocyanin production throughout plant. This gene, including its upstream promoter, coding region and terminator sequence, was introduced into apple, strawberry potato plants to determine whether it could be used as a visible selectable marker for plant transformation an alternative chemically markers, such kanamycin resistance. After transformation, red coloured calli, shoots well-growing were scored. Red green...
The pentacyclic triterpenes, in particular ursolic acid and oleanolic their derivatives, exist abundantly the plant kingdom, where they are well known for anti‐inflammatory, antitumour antimicrobial properties. α‐Amyrin β‐amyrin precursors of acids, respectively, formed by concerted cyclization squalene epoxide a complex synthase reaction. We identified three full‐length expressed sequence tag sequences cDNA libraries constructed from apple ( Malus × domestica ‘Royal Gala’) that were likely...
A pandemic isolate of Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) has devastated kiwifruit orchards growing cultivars Actinidia chinensis . In contrast, arguta (kiwiberry) is not a host Psa3. Resistance mediated via effector-triggered immunity, as demonstrated by induction the hypersensitive response in infected leaves, observed microscopy and quantified ion-leakage assays. Isolates Psa3 that cause disease have been isolated analyzed, revealing 51 kb deletion exchangeable effector...
Testing effector knockout strains of the Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) for reduced in planta growth their native kiwifruit host revealed a number nonredundant effectors that contribute to Psa3 virulence. Conversely, complementation weak pathogen P. actinidifoliorum (Pfm) increased identified redundant effectors. hopAZ1a and HopS2b entire exchangeable locus (ΔEEL; 10 effectors) were significant contributors bacterial colonisation additive effects on Four EEL (HopD1a,...
Outside Front Cover: The cover image is based on the article Identification and Characterization of Innate Immunity in Actinidia melanandra Response to Pseudomonas syringae pv. actinidiae by Jay Jayaraman et al., https://doi.org/10.1111/pce.15189.
In apple, the MYB transcription factor MYB10 controls accumulation of anthocyanins. is able to auto-activate its expression by binding own promoter at a specific motif, R1 motif. some apple accessions natural mutation, termed R6, has more copies this motif within resulting in stronger auto-activation and elevated Here we show that other anthocyanin-related MYBs, selected from pear, strawberry, petunia, kiwifruit, Arabidopsis, are activate promoters containing R6 To examine specificity...
Pseudomonas syringae pv. actinidiae (Psa) biovar 3, a virulent, canker-inducing pathogen is an economic threat to the kiwifruit (Actinidia spp.) industry worldwide. The commercially grown diploid (2×) A. chinensis var. more susceptible Psa than tetraploid and hexaploid kiwifruit. However information on genetic loci modulating resistance in not available. Here we report mapping of quantitative trait (QTLs) regulating population, derived from cross between elite Psa-susceptible 'Hort16A'...
Polyploidy is a key driver of significant evolutionary changes in plant species. The genus Actinidia (kiwifruit) exhibits multiple ploidy levels, which contribute to novel fruit traits, high yields and resistance the canker-causing dieback disease incited by Pseudomonas syringae pv. actinidiae (Psa) biovar 3. However, genetic mechanism for Psa observed polyploid kiwifruit not yet known. In this study we performed detailed analysis tetraploid chinensis var. population derived from cross...
DATA REPORT article Front. Genet., 16 May 2022Sec. Plant Genomics https://doi.org/10.3389/fgene.2022.852161
PTI and ETI are the two major defence mechanisms in plants. is triggered by detection of pathogen effectors, or their activity, plant cell most time involves internal receptors known as resistance (R) genes. An increasing number R genes responsible for recognition specific effectors have been characterised over years; however, methods to identify often challenging cannot always be translated crop plants.We present a novel method that trigger hypersensitive response (HR) Nicotiana...
The infection of Pseudomonas syringae pv. actinidiae in kiwifruit is currently assessed by numerous methodologies, each with their own limitations. Most studies are based on either a laborious method growth quantification the pathogen or qualitative assessments visual scoring following stem cutting inoculation. Additionally, when assessing for resistance against specific effectors, confounding interactions between multiple genes can make mapping phenotypes nearly impossible. Here, we present...
Summary Members of the hopQ1/XopQ family effectors are conserved amongst pathogenic bacteria suggesting an important function in virulence. Therefore, identification R proteins recognising members this is potentially high value to achieve broad-spectrum resistance crops. Recent studies demonstrated that HopQ1/XopQ recognition EDS1-dependant and mediated by TIR-NB-LRR protein Roq1. Using our recently described gene RNAi library, we have investigated mechanism HopQ1 other components its...
Abstract Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) has decimated kiwifruit orchards growing susceptible Actinidia chinensis varieties. Effector loss occurred recently in Psa3 isolates from resistant germplasm, resulting strains capable of partially overcoming resistance present kiwiberry vines ( A. arguta, polygama , and melanandra ). Diploid male recognises several effectors, sharing recognition at least one avirulence effector (HopAW1a) with previously studied tetraploid vines....
Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) causes a devastating canker disease in yellow-fleshed kiwifruit (Actinidia chinensis). The effector HopZ5, which is present all isolates of Psa3 causing global outbreaks pandemic disease, triggers immunity Nicotiana benthamiana and not recognised susceptible A. chinensis cultivars. In search for N. nonhost resistance genes against we found that the nucleotide-binding leucine-rich repeat receptor NbPTR1 HopZ5. RPM1-interacting protein 4...
ABSTRACT Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) has decimated kiwifruit orchards growing susceptible Actinidia chinensis varieties. Effector loss occurred recently in Psa3 isolates from resistant germplasm, resulting strains capable of partially overcoming resistance present kiwiberry vines ( arguta , polygama and melanandra ). Diploid male A. recognises several effectors, sharing recognition at least one avirulence effector (HopAW1a) with previously studied tetraploid vines....