A5079354258- Plant Molecular Biology Research
- Plant Physiology and Cultivation Studies
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Flowering Plant Growth and Cultivation
- Plant and animal studies
- Plant Gene Expression Analysis
- Plant Pathogens and Fungal Diseases
- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
- Postharvest Quality and Shelf Life Management
- Botanical Research and Applications
- Wheat and Barley Genetics and Pathology
- Horticultural and Viticultural Research
- Seedling growth and survival studies
- Plant biochemistry and biosynthesis
- Fern and Epiphyte Biology
- Ecology and Vegetation Dynamics Studies
- Animal Ecology and Behavior Studies
- Plant pathogens and resistance mechanisms
- Phytochemicals and Antioxidant Activities
- Polysaccharides and Plant Cell Walls
University of Canterbury
2015-2024
Yantai University
2019-2022
Massey University
1998-2009
AgResearch
2004
Plant & Food Research
2001
University of Otago
1985-1997
Institute of Plant Biology and Biotechnology
1996
University of Missouri
1989
Plants require sophisticated regulatory mechanisms to ensure the degree of anthocyanin pigmentation is appropriate myriad developmental and environmental signals. Central this process are activity MYB-bHLH-WD repeat (MBW) complexes that regulate transcription genes. In study, gene network regulates synthesis in petunia (Petunia hybrida) has been characterized. Genetic molecular evidence show R2R3-MYB, MYB27, an repressor functions as part MBW complex represses through its C-terminal EAR...
We present an investigation of anthocyanin regulation over the entire petunia plant, determining mechanisms governing complex floral pigmentation patterning and environmentally induced vegetative synthesis. DEEP PURPLE (DPL) HAZE (PHZ) encode members R2R3-MYB transcription factor family that regulate synthesis in petunia, control production tissues contribute to pigmentation. In addition these two MYB factors, basic helix-loop-helix (bHLH) ANTHOCYANIN1 (AN1) WD-repeat protein AN11, are also...
The Lc petunia system, which displays enhanced, light-induced vegetative pigmentation, was used to investigate how high light affects anthocyanin biosynthesis, and assess the effects of pigmentation upon photosynthesis. plants displayed intense purple throughout leaves stems when grown under high-light conditions, yet remain acyanic shade conditions. coloured phenotypes matched with an accumulation anthocyanins flavonols, as well activation early late flavonoid biosynthetic genes required...
Although simultaneous drought and cold stress occurs, especially in northwestern eastern regions of China, is an important factor limiting agricultural productivity, there are few studies focusing on plant responses to a combination stress. Here, by partially overlapping stresses, we characterized the acclimation maize (Zea mays B73) these two stresses using physiological measurements, as well comparative transcriptomics combined with metabolomics hormonal analyses during treatments recovery...
• Pigment stripes associated with veins (venation) is a common flower colour pattern. The molecular genetics and function of venation were investigated in the genus Antirrhinum, which determined by Venosa (encoding an R2R3MYB transcription factor). Pollinator preferences measured field tests Antirrhinum majus. was examined using situ hybridization transient overexpression. origin trait phylogenetics. Venation full-red colouration provide comparable level advantage for pollinator attraction...
Abstract Background Carotenoids and anthocyanins are the predominant non-chlorophyll pigments in plants. However, certain families within order Caryophyllales produce another class of pigments, betalains, instead anthocyanins. The occurrence betalains is mutually exclusive. Betalains divided into two classes, betaxanthins betacyanins, which yellow to orange or violet colours, respectively. In this article we show betalain production species that normally anthocyanins, through a combination...
While various labs had shown cell division-inducing activity in a variety of plant extracts for over decade, the identification zeatin (Z) 1964, first known naturally occurring cytokinin, belongs to Letham and co-workers. Using from maize (Zea mays), they were obtain crystals pure Z sufficient quantity structural determination by MS, NMR, chromatography, mixed melting-point analysis. This group also crystallized Z-9-riboside (ZR) coconut (Cocos nucifera) milk. However, their chemical...
Activities of enzymes involved in the detoxification reactive oxygen species (catalase, glutathione reductase, peroxidase and superoxide dismutase (SOD)) were examined leaves Phaseolus vulgaris L. var. Top Crop treated with plant hormones infected a non-lesion-forming isolate white clover mosaic potexvirus (WClMV). The activities catalase, reductase SOD rapidly declined after infection while activity was enhanced. These changes occurred before rapid increase (5 days) WClMV replication. A...
Abstract Background There is considerable interest in rapid assays or screening systems for assigning gene function. However, analysis of function the flowers some species restricted due to difficulty producing stably transformed transgenic plants. As a result, experimental approaches based on transient expression are frequently used. Biolistics has long been used over-expression genes interest, but not exploited silencing studies. Agrobacterium -infiltration also used, focus primarily...
• Background and Aims The xylem in fruit of a number species becomes dysfunctional as the develops, resulting reduction inflow to fruit. Such may have consequential effects on mineral balance aim this study was elucidate dynamics nature failure developing apples (Malus domestica) showing differing susceptibilities bitter pit, calcium‐related disorder.
The cytokinin group of plant hormones regulates aspects growth and development, including the release lateral buds from apical dominance delay senescence. In this work native promoter a synthase gene (ipt) was removed replaced with Cu-controllable promoter. Tobacco (Nicotiana tabacum L. cv tabacum) transformed Cu-inducible ipt (Cu-ipt) morphologically identical to controls under noninductive conditions in almost all lines produced. However, three grew an altered state, which is indicative...
As the global population continues to expand, increasing yield in bread wheat is of critical importance as 20% world's food supply sourced from this cereal. Several recent studies molecular basis grain indicate that cytokinins are a key factor determining yield. In study, cytokinin gene family members were isolated four multigene families which regulate synthesis and metabolism, isopentenyl transferases (IPT), oxidases (CKX), zeatin O-glucosyltransferases (ZOG), β-glucosidases (GLU)....
In a wheat breeding line XQ-0508 showing consistent resistance to powdery mildew disease, recessive gene, designated PmXQ-0508, was identified and mapped distal region on chromosome arm 2BS. Of three resistance-associated genes in this region, one encoding protein kinase selected as the primary candidate for PmXQ-0508. Ten closely linked DNA markers developed study could be used marker-assisted selection powdery-mildew programs.
When [(3)H]dihydrozeatin riboside and [(3)H]zeatin were supplied to soybean (Glycine max L.) explants (comprising one leaf, associated pods, subtending stem) via the xylem at mid late podfill, 0.1% of (3)H was extracted from seeds. The distribution in similar that bound previously following uptake earlier stages pod development. Metabolites formed (3)H-labeled zeatin, zeatin riboside, dihydrozeatin identified related endogenous cytokinins shown be present. for 1 hour, nucleotide principal...
Isolation of high quality DNA from multiple samples can be both time-consuming and expensive. We have developed a combined protocol to reduce the time component hexadecyltrimethylammonium bromide (CTAB) extraction method reduced costs by regenerating silica columns used purify genomic DNA. present data that shows, increasing temperature during CTAB method, required extract crude reduced. show regenerated using HCl still maintain their DNA-binding capacity. Furthermore, we...
Forage brassica (Brassica napus cv. Greenland) is bred for vegetative growth and biomass production, while its seed yield remains to be improved producers without affecting forage quality. Cytokinins affect by influencing flower, silique number, size. To identify specific cytokinin gene family members as targets breeding, well genes associated with and/or quality, a B. transcriptome was obtained from mixed sample including leaves, flower buds siliques of various stages. Gene families...
Abstract Leaves are colonized by a complex mix of microbes, termed the leaf microbiota. Even though microbiota is increasingly recognized as an integral part plant life and health, our understanding its interactions with host still limited. Here, mature, axenically grown Arabidopsis thaliana plants were spray inoculated six diverse leaf‐colonizing bacteria. The transcriptomic changes in leaves tracked over time significant ethylene marker ( ARL2 ) expression observed only 2–4 days after...