A5085155947- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Fungal Diseases
- Plant pathogens and resistance mechanisms
- Plant Parasitism and Resistance
- Banana Cultivation and Research
- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- Quality and Supply Management
- Plant Pathogens and Resistance
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Agricultural pest management studies
- Plant Disease Resistance and Genetics
- Insect-Plant Interactions and Control
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
Plant & Food Research
2018-2025
Pohang University of Science and Technology
2017-2020
Massey University
2015-2018
SUMMARY Arabidopsis thaliana WRKY proteins are potential targets of pathogen‐secreted effectors. RESISTANT TO RALSTONIA SOLANACEARUM 1 (RRS1; AtWRKY52) is a well‐studied nucleotide‐binding and leucine‐rich repeat (NLR) immune receptor carrying C‐terminal domain that functions as an integrated decoy. RRS1‐R recognizes the effectors AvrRps4 from Pseudomonas syringae pv. pisi PopP2 Ralstonia pseudosolanacearum by direct interaction through its domain. were previously shown to interact with...
Pseudomonas syringae is a widespread bacterial species complex that includes number of significant plant pathogens. Amongst these, P. pv. actinidiae (Psa) initiated worldwide pandemic in 2008 on cultivars Actinidia chinensis var. chinensis. To gain information about the expression genes involved pathogenicity we have carried out transcriptome analysis Psa during early stages kiwifruit infection.Gene was investigated first five days after infection plantlets, using RNA-seq. Principal...
Type-III secreted effectors (T3Es) play critical roles during bacterial pathogenesis in plants. Plant recognition of certain T3Es can trigger defence, often accompanied by macroscopic cell death, termed the hypersensitive response (HR). Economically important species kiwifruit are susceptible to Pseudomonas syringae pv. actinidiae (Psa), causal agent canker. Although Psa is non-pathogenic Arabidopsis thaliana, we observed that a T3E, HopZ5 unique global outbreak clade Psa, triggers HR and...
Abstract Bacterial pathogens are major causes of crop diseases, leading to significant production losses. For instance, kiwifruit canker, caused by the phytopathogen Pseudomonas syringae pv. actinidiae ( Psa ), has posed a global challenge production. Treatment with copper and antibiotics, whilst initially effective, is rise bacterial resistance, requiring new biocontrol approaches. Previously, we isolated group closely related phages potential, which represent environmentally sustainable...
A pandemic isolate of Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) has devastated kiwifruit orchards growing cultivars Actinidia chinensis . In contrast, arguta (kiwiberry) is not a host Psa3. Resistance mediated via effector-triggered immunity, as demonstrated by induction the hypersensitive response in infected leaves, observed microscopy and quantified ion-leakage assays. Isolates Psa3 that cause disease have been isolated analyzed, revealing 51 kb deletion exchangeable effector...
Testing effector knockout strains of the Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) for reduced in planta growth their native kiwifruit host revealed a number nonredundant effectors that contribute to Psa3 virulence. Conversely, complementation weak pathogen P. actinidifoliorum (Pfm) increased identified redundant effectors. hopAZ1a and HopS2b entire exchangeable locus (ΔEEL; 10 effectors) were significant contributors bacterial colonisation additive effects on Four EEL (HopD1a,...
Outside Front Cover: The cover image is based on the article Identification and Characterization of Innate Immunity in Actinidia melanandra Response to Pseudomonas syringae pv. actinidiae by Jay Jayaraman et al., https://doi.org/10.1111/pce.15189.
In 2010, a Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) incursion into New Zealand kiwifruit orchards devastated susceptible Actinidia chinensis cultivars. contrast, many species maintained in germplasm collections were resistant to Psa3 and showed limited symptoms. Recent genome biosurveillance revealed the emergence of widespread leaf spot symptoms Psa3-resistant germplasm. Surprisingly, few isolates recovered from symptomatic tissues, despite frequent isolation phenotypical on...
In the late 2000s, a pandemic of Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) devastated kiwifruit orchards growing susceptible, yellow-fleshed cultivars. New Zealand's industry has since recovered, following deployment tolerant cultivar 'Zesy002'. However, little is known about extent to which Psa population evolving its arrival. Over 500 Psa3 isolates from Zealand were sequenced between 2010 and 2022, commercial monocultures diverse germplasm collections. While effector loss was...
Bacterial phytopathogen type III secreted (T3S) effectors have been strongly implicated in altering the interaction of pathogens with host plants. Therefore, it is useful to characterize whole effector repertoire a pathogen understand interplay Pseudomonas syringae pv. actinidiae causal agent kiwifruit canker disease. In this study, we generated an Agrobacterium-mediated transient expression library YFP-tagged T3S from two strains Psa, Psa-NZ V13 and LV5, order gain insight into their mode...
Ralstonia solanacearum (Rso) is a causal agent of bacterial wilt in Solanaceae crops worldwide including Republic Korea. Rso virulence predominantly relies on type III secreted effectors (T3Es). However, only handful T3Es have been characterized. In this study, we investigated subcellular localization and manipulation plant immunity by 8 predicted to harbor nuclear signal (NLS). While 2 these elicited cell death both Nicotiana benthamiana N. tabacum, one was dependent suppressor G2 allele...
Pseudomonas syringae pv. actinidiae ICMP 18884 biovar 3 (Psa3) produces necrotic lesions during infection of its kiwifruit host. Bacterial growth in planta and lesion formation are dependent upon a functional type III secretion system (T3S), which translocates multiple effector proteins into host cells. Associated with the T3S locus is conserved (CEL), has been characterized shown to be essential for full virulence other P. pathovars. Two effectors at CEL, hopM1 avrE1, as well an...
Summary The common polysaccharide antigen (CPA) of the lipopolysaccharide (LPS) from Pseudomonas syringae is highly variable, but genetic basis for this poorly understood. We have characterized CPA locus P. pv. actinidiae ( Psa ). This has genes l ‐ and d ‐rhamnose biosynthesis an operon coding ABC transporter subunits, a bifunctional glycosyltransferase o ‐methyltransferase. predicted to role in transport, elongation termination oligosaccharide referred as TET operon. Two alleles were...
The infection of Pseudomonas syringae pv. actinidiae in kiwifruit is currently assessed by numerous methodologies, each with their own limitations. Most studies are based on either a laborious method growth quantification the pathogen or qualitative assessments visual scoring following stem cutting inoculation. Additionally, when assessing for resistance against specific effectors, confounding interactions between multiple genes can make mapping phenotypes nearly impossible. Here, we present...
Bacterial wilt caused by the Ralstonia solanacearum species complex (RSSC) threatens cultivation of important crops worldwide. We sequenced 30 RSSC phylotype I ( R. pseudosolanacearum ) strains isolated from pepper Capsicum annuum and tomato Solanum lycopersicum across Republic Korea. These isolates span diversity I, have extensive effector repertoires are subject to frequent recombination. Recombination hotspots among South Korean include multiple predicted contact-dependent inhibition...
Ralstonia solanacearum is the causal agent of devastating bacterial wilt disease in many high value Solanaceae crops. R. secretes around 70 effectors into host cells order to promote infection. Plants have, however, evolved specialized immune receptors that recognize corresponding and confer qualitative resistance. In model species Arabidopsis thaliana, paired RRS1 (resistance 1) RPS4 Pseudomonas syringae 4) cooperatively effector PopP2 nuclei infected cells. an acetyltransferase binds...
Summary Plants evolved disease resistance (R) proteins that recognize corresponding pathogen effectors and activate effector‐triggered immunity ( ETI ). However, it is largely unknown why, in some cases, a suppressor of exists plants. Arabidopsis SOBER 1 (Suppressor AvrBsT‐elicited Resistance 1) was identified previously as Xanthomonas acetyltransferase effector AvrBsT‐triggered immunity. Nevertheless, the extent to which suppresses unclear. Here, we Pseudomonas HopZ5‐triggered using...
Abstract Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) has decimated kiwifruit orchards growing susceptible Actinidia chinensis varieties. Effector loss occurred recently in Psa3 isolates from resistant germplasm, resulting strains capable of partially overcoming resistance present kiwiberry vines ( A. arguta, polygama , and melanandra ). Diploid male recognises several effectors, sharing recognition at least one avirulence effector (HopAW1a) with previously studied tetraploid vines....
Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) causes a devastating canker disease in yellow-fleshed kiwifruit (Actinidia chinensis). The effector HopZ5, which is present all isolates of Psa3 causing global outbreaks pandemic disease, triggers immunity Nicotiana benthamiana and not recognised susceptible A. chinensis cultivars. In search for N. nonhost resistance genes against we found that the nucleotide-binding leucine-rich repeat receptor NbPTR1 HopZ5. RPM1-interacting protein 4...
ABSTRACT Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) has decimated kiwifruit orchards growing susceptible Actinidia chinensis varieties. Effector loss occurred recently in Psa3 isolates from resistant germplasm, resulting strains capable of partially overcoming resistance present kiwiberry vines ( arguta , polygama and melanandra ). Diploid male A. recognises several effectors, sharing recognition at least one avirulence effector (HopAW1a) with previously studied tetraploid vines....
Ralstonia solanacearum causes bacterial wilt disease in solanaceous crops. Identification of avirulence type III-secreted effectors recognized by specific resistance proteins host plant species is an important step toward developing durable In the present study, we show that R. effector RipJ functions as determinant Solanum pimpinellifolium LA2093. all, 10 candidate were shortlisted based on repertoire comparison between avirulent Pe_9 and virulent Pe_1 strains. Infection assays with...