A5022627638- Plant Pathogenic Bacteria Studies
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogens and Fungal Diseases
- Cancer Genomics and Diagnostics
- Plant-Microbe Interactions and Immunity
- Cocoa and Sweet Potato Agronomy
- Molecular Biology Techniques and Applications
- Plant Virus Research Studies
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Legume Nitrogen Fixing Symbiosis
- Transgenic Plants and Applications
- Plant pathogens and resistance mechanisms
- T-cell and B-cell Immunology
- CRISPR and Genetic Engineering
- Biosensors and Analytical Detection
- Genomic variations and chromosomal abnormalities
- Insect symbiosis and bacterial influences
- Banana Cultivation and Research
- Cancer Immunotherapy and Biomarkers
- Plant tissue culture and regeneration
- Single-cell and spatial transcriptomics
- Genomics and Phylogenetic Studies
- Hymenoptera taxonomy and phylogeny
- Antimicrobial Resistance in Staphylococcus
Plant & Food Research
2012-2025
Thermo Fisher Scientific (United States)
2015-2023
The University of Texas Southwestern Medical Center
2017
IRCCS Policlinico San Donato
2017
University of Milan
2017
Manaaki Whenua – Landcare Research
2013
CrossLife Technologies (United States)
2012
AgResearch
1998-2006
Danish Ministry of Food, Agriculture and Fisheries
2005
The origins of crop diseases are linked to domestication plants. Most crops were domesticated centuries – even millennia ago, thus limiting opportunity understand the concomitant emergence disease. Kiwifruit (Actinidia spp.) is an exception: began in 1930s with outbreaks canker disease caused by P. syringae pv. actinidiae (Psa) first recorded 1980s. Based on SNP analyses two circularized and 34 draft genomes, we show that Psa comprised distinct clades exhibiting negligible within-clade...
Phormium yellow leaf (PYL) phytoplasma causes a lethal disease of the monocotyledon, New Zealand flax (Phormium tenax). The 16S rRNA genes PYL were amplified from infected by PCR and cloned, nucleotide sequences determined. DNA sequencing Southern hybridization analysis genomic indicated presence two copies gene. exhibited sequence heterogeneity in 4 positions could be distinguished restriction enzymes BpmI BsrI. This is first record which has been determined analysis. A phylogenetic tree...
For unknown reasons, there is huge variability in risk conferred by different HPV types and, remarkably, strong differences even between closely related variant lineages within each type. HPV16 a uniquely powerful carcinogenic type, causing approximately half of cervical cancer and most other HPV-related cancers. To permit the large-scale study genome precancer/cancer, starting with cancer, we developed high-throughput next-generation sequencing (NGS) whole-genome method. We designed custom...
'Candidatus Phytoplasma australiense' is associated with at least nine diseases in Australia and New Zealand. The impact of this phytoplasma considerable, both economically environmentally. genome a NZ isolate was sequenced an effort to understand its pathogenicity ecology. Comparison closely related Australian enabled us examine mechanisms genomic rearrangement. complete sequence strawberry lethal yellows (SLY) determined. It circular 959,779 base pairs 1126 predicted open reading frames....
White clover mosaic virus strain O (WClMV-O), species of the Potexvirus genus, contains a set three partially overlapping genes (the triple gene block) that encodes nonvirion proteins 26 kDa, 13 and 7 kDa. These are necessary for cell-to-cell movement in plants but not replication. The WClMV-O 13-kDa was mutated (to 13*) region is conserved all viruses known to possess triple-gene-block proteins. All 10 13* transgenic lines Nicotiana benthamiana designed express protein were shown be...
A pandemic isolate of Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) has devastated kiwifruit orchards growing cultivars Actinidia chinensis . In contrast, arguta (kiwiberry) is not a host Psa3. Resistance mediated via effector-triggered immunity, as demonstrated by induction the hypersensitive response in infected leaves, observed microscopy and quantified ion-leakage assays. Isolates Psa3 that cause disease have been isolated analyzed, revealing 51 kb deletion exchangeable effector...
In the late 2000s, a pandemic of Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) devastated kiwifruit orchards growing susceptible, yellow-fleshed cultivars. New Zealand's industry has since recovered, following deployment tolerant cultivar 'Zesy002'. However, little is known about extent to which Psa population evolving its arrival. Over 500 Psa3 isolates from Zealand were sequenced between 2010 and 2022, commercial monocultures diverse germplasm collections. While effector loss was...
To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to method assessing co-colonization pigs by spp.The specificity developed 5' nuclease rt-PCR species-specific identification jejuni, coli, lari, upsaliensis hipO gene nucleotide probe detection C. jejuni colony-blot hybridization were determined testing total 75 reference strains spp. related organisms. The allowed in naturally infected pig faecal samples after an enrichment step, whereas the...
Pseudomonas syringae pv. actinidiae is the causal agent of bacterial canker kiwifruit, a disease that has rapidly spread worldwide. We have fully sequenced and assembled chromosomal plasmid DNA from P. ICMP 18884 using PacBio RS II platform.
Pseudomonas syringae pv. actinidiae (Psa), the causal agent of kiwifruit canker, is one most devastating plant diseases recent times. We have generated two mini-Tn5-based random insertion libraries Psa ICMP 18884. The first, a 'phenotype interest' (POI) library, consists 10,368 independent mutants gridded into 96-well plates. By replica plating onto selective media, POI library was successfully screened for auxotrophic and motility mutants. Lipopolysaccharide (LPS) biosynthesis with...
Pseudomonas syringae pv. actinidiae (Psa) is responsible for bacterial canker of kiwifruit. Biovar 3 Psa (Psa3) has been causing widespread damage to yellow‐ and green‐fleshed kiwifruit ( Actinidia spp.) cultivars in all the major kiwifruit‐producing countries world. In some areas, including New Zealand, P. actinidifoliorum (Pfm), another pathogen kiwifruit, was initially classified as a low virulence biovar Psa. Ability rapidly distinguish between these pathovars vital management canker....
A yellows disease of strawberry plants was identified in propagation beds New Zealand. Affected were flatter to the ground, showed purpling older leaves, reduced leaf size, yellowing younger and sometimes plant death. phytoplasma observed phloem affected plants. The 16S rRNA gene amplified by polymerase chain reaction from symptomatic one asymptomatic plant, but not 36 other Nucleotide sequence analysis that is closely related or identical associated with yellow Zealand flax (Phormium tenax).
Sudden decline of the New Zealand cabbage tree (Cordyline australis) results in rapid death affected plants within months first external symptoms becoming apparent. Symptoms, which have been observed saplings and mature trees, include vascular discoloration leaf yellowing followed by desiccation eventual plant collapse. Previous work failed to link disease with any causal agent. A phytoplasma has now detected all symptomatic some trees tested, using one-step nested polymerase chain reaction...
A reliable diagnostic method was developed for use in studying the relationship between phormium yellow leaf disease of New Zealand flax ( Phormium tenax ) and its associated phytoplasma (phormium phytoplasma: PYL). Diagnosis involved a nested PCR (polymerase chain reaction) technique targeting 16S rRNA gene. DNA extracted from woody rhizome tissues NZ plants using CTAB high salt precipitation step. This effectively eliminated polysaccharides, gum‐like material other compounds inhibitory to...
Abstract The history of Boysenberry (Rubus ursinus Chamisso & Schlenhtendal) and Youngberry (R. derivative) were traced from their origins in the United States, to importation into New Zealand, subsequent use as major Rubus crops. investigation possible causes decline disease are described, probable association recently detected Cercosporella rubi fungus with this is discussed, presence Phormium yellow leaf phytoplasma some affected plants. A graft‐transmissible agent Boysenberries, which...
'Candidatus Phytoplasma australiense' is associated with a number of plant diseases in New Zealand. The only known vector this pathogen was Zeoliarus atkinsoni, planthopper considered to be monophagous on Zealand flax (Phormium spp.). work carried out shows that Z. oppositus, which polyphagous, able 'Ca. P. both Coprosma robusta (karamu) and Cordyline australis (New cabbage tree). Although transmission achieved these species, the disease symptomatology more evident C. australis. Two...
The phytoplasma “Candidatus Phytoplasma australiense” has been reported from New Zealand and Australia, where it associated with a range of host plants, especially since the 1970s. Partial tuf gene sequences 36 (NZ) isolates four different genera revealed nine variants, which clustered into two distinct groups without any obvious correlation or geographic region. Phylogenetic analysis these sequences, together those available Australian isolates, three clades: one found solely in NZ, third...