A5058170779- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Listeria monocytogenes in Food Safety
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Genetic and phenotypic traits in livestock
- Antibiotic Resistance in Bacteria
- Milk Quality and Mastitis in Dairy Cows
- Veterinary medicine and infectious diseases
- Food Safety and Hygiene
- Vibrio bacteria research studies
- Clostridium difficile and Clostridium perfringens research
- Identification and Quantification in Food
- Indoor Air Quality and Microbial Exposure
- Effects of Environmental Stressors on Livestock
- Probiotics and Fermented Foods
- Gut microbiota and health
- Pharmaceutical and Antibiotic Environmental Impacts
- Odor and Emission Control Technologies
- Genomics and Phylogenetic Studies
- Air Quality and Health Impacts
- Enterobacteriaceae and Cronobacter Research
- Animal Nutrition and Physiology
- Aquaculture disease management and microbiota
- Essential Oils and Antimicrobial Activity
Statens Serum Institut
2016-2025
Herlev Hospital
2024
Technical University of Denmark
1998-2021
University of Copenhagen
2001-2017
Frederiksberg Hospital
2017
University of Southern Denmark
2016
Aarhus University Hospital
2007-2008
Danish Ministry of Food, Agriculture and Fisheries
2004-2007
Aalborg University Hospital
2007
Regionshospitalet Viborg
2005
Fast and accurate identification typing of pathogens are essential for effective surveillance outbreak detection. The current routine procedure is based on a variety techniques, making the laborious, time-consuming, expensive. With whole-genome sequencing (WGS) becoming cheaper, it has huge potential in both diagnostics surveillance. aim this study was to perform real-time evaluation WGS verocytotoxin-producing Escherichia coli (VTEC). In Denmark, Statens Serum Institut (SSI) routinely...
The Escherichia coli strain causing a large outbreak of haemolytic uraemic syndrome and bloody diarrhoea in Germany May June 2011 possesses an unusual combination pathogenic features typical enteroaggregative E. together with the capacity to produce Shiga toxin. Through rapid national international exchange information strains known occurrence humans was quickly assessed.We describe simple diagnostic screening tools detect clinical specimens novel real-time PCR for its detection foods.
National Salmonella surveillance systems from France, England and Wales, Denmark, the United States identified recent emergence of multidrug-resistant isolates enterica serotype Kentucky displaying high-level resistance to ciprofloxacin. A total 489 human cases were during period 2002 (3 cases) 2008 (174 cases). These belonged a single clone defined by multilocus sequence type ST198, XbaI-pulsed-field gel electrophoresis cluster X1, presence genomic island 1 variant SGI1-K. This was probably...
Salmonella enterica is a common cause of minor and large food borne outbreaks. To achieve successful nearly 'real-time' monitoring identification outbreaks, reliable sub-typing essential. Whole genome sequencing (WGS) shows great promises for using as routine epidemiological typing tool. Here we evaluate WGS S. Typhimurium including different approaches analyzing comparing the data. A collection 34 isolates was sequenced. This consisted 18 from six outbreaks 16 epidemiologically unrelated...
Abstract We report the findings of a case-control study risk factors for sporadic cases human campylobacteriosis in Denmark. In 3 different analytical models, main domestic factor identified was eating fresh, unfrozen chicken. Specifically, 28 74 domestically acquired case-patients were exposed to fresh chicken compared with 21 114 controls (multivariate matched odds ratio 5.8; 95% confidence interval 2.1–15.9). contrast, from other poultry, including previously frozen chicken, only...
The number of human cases enteritis caused by Campylobacter jejuni and C. coli is increasing in Denmark other European countries. No systemic typing has earlier been performed on isolates Danish origin. primary purpose this study was to provide a serotype distribution from patients the major food production animals. In addition, occurrence intestinal carriers thermophilic campylobacters among these animals examined. nationwide survey, individual isolation rate 36% for broiler chickens, 47%...
ABSTRACT In recent years increased attention has been focused on infections caused by isolates of verocytotoxin-producing Escherichia coli (VTEC) serotypes other than O157. These non-O157 VTEC are commonly present in food and production animals. Easy detection, isolation, characterization essential for improving our knowledge these organisms. the study, we detected bovine fecal samples a duplex 5′ nuclease PCR assay (real-time PCR) that targets vtx1 vtx2 . were obtained colony replication...
The MICs of 16 antimicrobial agents were determined for 202 Campylobacter jejuni isolates, 123 coli and 6 lari isolates from humans food animals in Denmark. C. originated (75), broilers (95), cattle (29), pigs (3); the (7), (17), (99); (5) (1). All susceptible to apramycin, neomycin, gentamicin. Only a few resistant one or more agents. Resistance tetracycline was common among (11%) than (0 2%). More resistance streptomycin found (10%) those (4%) (1%). A greater proportion other tested....
Multilocus variable number of tandem repeats analysis (MLVA) has recently become a widely used highly discriminatory molecular method for typing the foodborne pathogen Salmonella Typhimurium. This is based on amplification and fragment size five repeat loci. To be able to easily compare MLVA results between laboratories there need simple definitive nomenclature profiles. Based all human S. Typhimurium isolates in Denmark from last years sequence selection these isolates, we propose that...
We present the LiSEQ (Listeria SEQuencing) project, funded by European Food Safety Agency (EFSA) to compare Listeria monocytogenes isolates collected in Union from ready-to-eat foods, compartments along food chain (e.g. food-producing animals, food-processing environments) and humans. In this article, we report molecular characterization of a selection data set employing whole-genome sequencing analysis. an overview strain diversity observed different sampled sources, characterize based on...
Background. Listeriosis is a serious foodborne infection. Outbreaks of listeriosis occur rarely, but have often proved difficult to solve. In June 2014, we detected and investigated outbreak in Denmark using patient interviews whole-genome sequencing (WGS). Methods. We performed WGS on Listeria monocytogenes isolates from patients available ready-to-eat foods compared them single-nucleotide polymorphism (SNP) analysis. Case had L. with ≤3 SNPs (the strain) isolated September 2013−December...
The Klebsiella pneumoniae species complex (KpSC) is a leading cause of multidrug-resistant human infections. To better understand the potential contribution food as vehicle KpSC, we conducted multicentric study to define an optimal culture method for its recovery from matrices and characterize isolates phenotypically genotypically. Chicken meat (n = 160) salad 145) samples were collected in five European countries screened presence KpSC using culture-based zur-khe intergenic region (ZKIR)...
Abstract We integrated data on quinolone and macrolide susceptibility patterns with epidemiologic typing from Campylobacter jejuni C. coli infections in two Danish counties. The mean duration of illness was longer for 86 patients quinolone-resistant (median 13.2 days) than 381 quinolone-sensitive 10.3 days, p = 0.001). Foreign travel, eating fresh poultry other chicken turkey, swimming were associated increased risk infection. Eating (of presumably origin) a decreased risk. Typing showed an...
ABSTRACT Six methods for subtyping of Campylobacter jejuni were compared and evaluated with a collection 90 isolates from poultry, cattle, sporadic human clinical cases as well waterborne outbreak. The applied Penner heat-stable serotyping; automated ribotyping (RiboPrinting); random amplified polymorphic DNA typing (RAPD); pulsed-field gel electrophoresis (PFGE); restriction fragment length polymorphisms the flagellin gene, flaA ( fla -RFLP); denaturing gradient -DGGE). on basis their...
Aims: To study the prevalence of seven virulence and toxin genes, cytolethal distending (CDT) production Campylobacter jejuni C. coli isolates from Danish pigs cattle. Methods Results: The presence cadF, ceuE, virB11, flaA, cdtA, cdtB, cdtC cdt gene cluster among 40 was detected by polymerase chain reaction. CDT determined on Vero, colon 205 chicken embryo cells. ceuE cdtB genes were 100% isolates. cdtA found in 95·0 90·0% isolates, respectively. 82·5% Only 7·5% positive for virB11....
We describe here an examination of the validity molecular types Campylobacter jejuni as defined by separation SmaI-digested DNA using pulsed-field gel electrophoresis (PFGE), recently suggested part a subtyping scheme. Thirty-four Danish strains from humans, water, poultry and cattle were assigned to one six SmaI 'profile groups' (PGs), with two additional included genotypically distinct controls. The interstrain relationships reexamined PFGE SalI, KpnI BamHI-digested DNA, also serotyping...
We describe an outbreak of Shiga toxin-producing Escherichia coli O26:H11 infection in 20 patients (median age, 2 years). The source the was organic fermented beef sausage. discovered by using credit card information to obtain and compare customer transaction records from computer systems supermarkets.