A5055289464- Listeria monocytogenes in Food Safety
- Essential Oils and Antimicrobial Activity
- Salmonella and Campylobacter epidemiology
- Microbial Inactivation Methods
- French Urban and Social Studies
- Food Safety and Hygiene
- Yersinia bacterium, plague, ectoparasites research
- Identification and Quantification in Food
- Viral gastroenteritis research and epidemiology
- Agriculture and Rural Development Research
- Plant-based Medicinal Research
- Vibrio bacteria research studies
- Bacterial Identification and Susceptibility Testing
- Health, Medicine and Society
- Spectroscopy and Chemometric Analyses
- Zoonotic diseases and public health
- Social Sciences and Governance
- Enterobacteriaceae and Cronobacter Research
- Pharmacological Effects of Natural Compounds
- European Socioeconomic and Political Studies
- Diagnosis and Treatment of Venous Diseases
- Pesticide Residue Analysis and Safety
- Bacillus and Francisella bacterial research
- Aging, Elder Care, and Social Issues
- Dermatological diseases and infestations
Institut Pasteur
2015-2024
Université Paris-Saclay
2023-2024
Biology of Infection
2015-2024
UCLouvain Saint-Louis Brussels
2024
Invisible Software (United States)
2024
Leefmilieu Brussel
2024
Universidad Nacional
2023
Inserm
2011-2023
Université Paris Cité
2011-2023
Institut Necker Enfants Malades
2017-2023
Abstract Listeria monocytogenes ( Lm ) is a major human and animal foodborne pathogen. Here we show that hypervirulent clones, particularly CC1, are strongly associated with dairy products, whereas hypovirulent CC9 CC121, meat products. Clone adaptation to distinct ecological niches and/or different food products contamination routes may account for this uneven distribution. Indeed, clones colonize better the intestinal lumen invade more tissues than ones, reflecting their adaption host...
We conducted epidemiologic, trace-back, and environmental investigations used whole-genome sequencing to type Listeria monocytogenes isolates. A case was defined as laboratory-confirmed L. infection during the period from June 11, 2017, April 7, 2018.
ABSTRACT Susceptibility to antibiotics of 4,816 clinical L. monocytogenes strains isolated since 1926 was studied, and the temporal evolution susceptibility analyzed through several decades. The mechanisms resistance in each resistant strain were studied. prevalence estimated at 1.27% among isolates from humans. Resistance tetracyclines+ fluoroquinolones more common has recently emerged. Although acquired did not implicate clinically relevant antibiotics, possibility gene transfers,...
Abstract Two species of Listeria are pathogenic; L. monocytogenes infects humans and animals, ivanovii has been considered to infect ruminants only. We report ivanovii–associated gastroenteritis bacteremia in a man. This isolate was indistinguishable from prototypic ruminant strains. is thus an enteric opportunistic human pathogen.
A Listeria -like strain isolated in Austria from pre-cut lettuce fitted the description of genus although it could not be assigned to any known species. Comparison rrs gene (encoding 16S rRNA) sequence and content by DNA-array indicated affiliation . Phylogenetic distance species that represents a novel Since can differentiated all other using phenotypic tests, name rocourtiae sp. nov. is proposed for The type CIP 109804 T (=DSM 22097 =Allerberger 700284/02 ). avirulent as assessed cell...
Abstract Listeria monocytogenes is worldwide a pathogen, but the geographic distribution of clones remains largely unknown. Genotyping 300 isolates from 5 continents and diverse sources showed existence few prevalent globally distributed clones, some which include previously described epidemic clones. Cosmopolitan indicates need for genotyping standardization.
During 2015-2016, we evaluated the performance of whole-genome sequencing (WGS) as a routine typing tool. Its added value for microbiological and epidemiologic surveillance listeriosis was compared with that pulsed-field gel electrophoresis (PFGE), current standard method. A total 2,743 Listeria monocytogenes isolates collected part were characterized in parallel by PFGE core genome multilocus sequence (cgMLST) extracted from WGS. We investigated cgMLST clusters containing human isolates....
Matrix-associated laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a rapid and simple microbial identification method. Previous reports using the Biotyper system suggested that this technique requires preliminary extraction step to identify Gram-positive rods (GPRs), technical issue may limit routine use pathogenic GPRs in clinical setting. We tested accuracy MALDI-TOF MS Andromas strategy set 659 GPR isolates representing 16 bacterial genera 72 species by...
The food-borne pathogen Listeria monocytogenes is genetically heterogeneous. Although some clonal groups have been implicated in multiple outbreaks, there currently no consensus on how "epidemic clones" should be defined. objectives of this work were to compare the patterns sequence diversity two sets genes that widely used define L. groups: multilocus typing (MLST) and multi-virulence-locus (MvLST). Further, we evaluated within by pulsed-field gel electrophoresis (PFGE). Based 125 isolates...
ABSTRACT The pathogenesis of Listeria monocytogenes depends on the ability this bacterium to escape from phagosome host cells via action pore-forming toxin listeriolysin O (LLO). Expression LLO-encoding gene ( hly ) requires transcriptional activator PrfA, and both prfA genes are essential for L. virulence. Here, we used hemolytic activity LLO as a phenotypic marker screen spontaneous virulence-attenuating mutations in . Sixty nonhemolytic isolates were identified among collection 57,820...
Large-scale studies are needed to clarify antimicrobial resistance in the foodborne pathogen
Bacterial sepsis has become the most frequent infectious complication of transfusion. Although Yersinia enterocolitica is a common enteropathogen usually causing relatively mild disease, it nevertheless prominent cause life-threatening post-transfusion infection. To gain better understanding clinical presentation and prognosis this rare occurrence, we performed systematic detailed review 55 published cases, which present here after description mechanisms underlying contamination red blood...
In August 2017, an outbreak of six listeriosis cases in Denmark was traced to cold-smoked salmon, using epidemiological investigations and whole-genome sequencing (WGS) analyses. Exchange genome sequences allowed identification France a food isolate from salmon-derived product human 2016 within the same cgMLST cluster as Danish isolates (L2-SL8-ST8-CT771). The salmon came third European Union country. WGS can rapidly link across Europe.
The effect of the bacteriocin-producing Lactobacillus sakei CRL1862 and its bacteriocin in control Listeria biofilm formation on industrial surfaces at 10°C was investigated. A screening among different species performed allowing selecting L. monocytogenes FBUNT for use as a producer stainless steel (SS) polytetrafluoroe-thylene (PTFE) surfaces. Three conditions were simulated to evaluate ability bacteriocinogenic strain displace, exclude compete pathogen formation. effectively inhibited by...
During a screening of Listeria species in food samples Thailand, Listeria-like bacterium was recovered from fried chicken and could not be assigned to any known species. Phylogenetic analysis based on the 16S rRNA gene 243 core genes placed novel taxon within aquatica, floridensis, fleishmannii costaricensis clade (Listeria sensu lato), with highest similarity L. floridensis (98.9 %) (98.8 %). Whole-genome sequence analyses average nucleotide blast identity (ANI<86 %), pairwise amino acid...
Abstract Invasive listeriosis is a severe foodborne infection in humans and difficult to control. Listeriosis incidence increasing worldwide, but some countries have implemented molecular surveillance programs improve recognition management of outbreaks. In Germany, routine whole-genome sequencing, core genome multilocus sequence typing, single nucleotide polymorphism calling are used for subtyping Listeria monocytogenes isolates from cases suspected foods. During 2018–2019, an unusually...
In the context of a study on occurrence Listeria species in an animal farm environment Valencia, Spain, six -like isolates could not be assigned to any known species. Phylogenetic analysis based 16S rRNA gene and 231 core genes grouped these monophyletic clade within genus , with highest similarity thailandensis . Whole-genome sequence analyses silico DNA–DNA hybridization, average nucleotide blast pairwise amino acid identities against all currently confirmed that constituted new taxon...
Listeria innocua is considered a nonpathogenic species. Natural atypical hemolytic L. isolates have been reported but not characterized in detail. Here, we report the genomic and functional characterization of representative from two known natural clades. Whole-genome sequencing confirmed presence pathogenicity islands (LIPI) characteristic monocytogenes Functional assays showed that LIPI-1 inlA genes are transcribed, corresponding gene products expressed functional. Using vitro vivo assays,...
The bacterial foodborne pathogen Listeria monocytogenes clonal complex 1 (Lm-CC1) is the most prevalent group associated with human listeriosis and strongly cattle dairy products. Here, we analyze 2021 isolates collected from 40 countries, covering Lm-CC1 first isolation to present days, define its evolutionary history population dynamics. We show that spread worldwide North America following Industrial Revolution through two waves of expansion, coinciding transatlantic livestock trade in...