A5013852775- Diphtheria, Corynebacterium, and Tetanus
- Bacillus and Francisella bacterial research
- Viral gastroenteritis research and epidemiology
- Bacterial Identification and Susceptibility Testing
- Probiotics and Fermented Foods
- Microbial Inactivation Methods
- Mycobacterium research and diagnosis
- Microbial bioremediation and biosurfactants
- Veterinary medicine and infectious diseases
- Streptococcal Infections and Treatments
- Salmonella and Campylobacter epidemiology
- Hepatitis Viruses Studies and Epidemiology
- Wastewater Treatment and Nitrogen Removal
- Microbial Metabolic Engineering and Bioproduction
- Environmental Chemistry and Analysis
- Bacteriophages and microbial interactions
- Yersinia bacterium, plague, ectoparasites research
- Polyamine Metabolism and Applications
- Identification and Quantification in Food
- Enzyme Catalysis and Immobilization
- Botulinum Toxin and Related Neurological Disorders
- Genomics and Phylogenetic Studies
- Food Safety and Hygiene
- Listeria monocytogenes in Food Safety
- Animal Virus Infections Studies
Chemisches und Veterinäruntersuchungsamt Stuttgart
2009-2023
University of Stuttgart
1996-2007
An aerobic endospore-forming bacillus (NVH 391-98(T)) was isolated during a severe food poisoning outbreak in France 1998, and four other similar strains have since been isolated, also mostly from cases. Based on 16S rRNA gene sequence similarity, these were shown to belong the Bacillus cereus Group (over 97% similarity with current species) phylogenetic distance validly described species of genus less than 95%. MLST data, novel form robust well-separated cluster B. Group, constituted most...
While the relevance of Bacillus (B.) cereus as a major cause gastroenteritis is undisputed, role closely related B. thuringiensis in foodborne disease unclear. strains frequently harbor enterotoxin genes. However, organism has only very rarely been associated with outbreaks, possibly due to fact that during outbreak investigations, routinely not differentiated from thuringiensis. A recent EFSA scientific opinion stresses urgent need for further data allowing improved risk assessment,...
Abstract Invasive listeriosis is a severe foodborne infection in humans and difficult to control. Listeriosis incidence increasing worldwide, but some countries have implemented molecular surveillance programs improve recognition management of outbreaks. In Germany, routine whole-genome sequencing, core genome multilocus sequence typing, single nucleotide polymorphism calling are used for subtyping Listeria monocytogenes isolates from cases suspected foods. During 2018–2019, an unusually...
A total of 34 Corynebacterium sp. strains were isolated from caseous lymph node abscesses wild boar and roe deer in different regions Germany. They showed slow growth on Columbia sheep blood agar sparse Hoyle's tellurite agar. Cellular fatty acid analysis allocated them the C. diphtheriae group genus Corynebacterium. MALDI-TOF MS using specific database extensions rpoB sequencing resulted classification as ulcerans. Their quinone system is similar to ulcerans, with major menaquinone...
A bacterial strain (strain S5) which grows aerobically with the sulfonated azo compound 4-carboxy-4'-sulfoazobenzene as sole source of carbon and energy was isolated. This obtained by continuous adaptation "Hydrogenophaga palleronii" S1, has ability to grow 4-aminobenzenesulfonate. Strain S5 probably cleaves reductively under aerobic conditions 4-aminobenzoate 4-aminobenzene-sulfonate, are mineralized previously established degradation pathways.
The genus Streptobacillus (S.) remained monotypic for almost 90 years until two new species were recently described. type species, S. moniliformis, is one of the etiological agents rat bite fever, an under-diagnosed, worldwide occurring zoonosis. In a polyphasic approach field isolates and reference strains hongkongensis, felis as well divergent characterized by comparison molecular data (n = 29) from majority also their physiological proteomic properties 22). Based on growth-independent...
ABSTRACT The identification and molecular epidemiology of norovirus in outbreaks gastroenteritis were studied during a 3-year period Germany. Specimens ( n = 316) from 159 nonbacterial March 2001 to June 2004 analyzed for the presence noroviruses by reverse transcriptase PCR. Outbreaks most frequent elderly people's homes care centers (43%), followed hospitals (24%). Molecular analyses strains 148 showed that there up 12 genotypes involved outbreaks. Genogroup II responsible 95%...
Corynebacterium ulcerans may cause diphtheria in humans and caseous lymphadenitis animals. We isolated nontoxigenic tox-bearing C. from 13 game animals Germany. Our results indicate a role for as reservoirs zoonotic ulcerans.
A pleomorphic, Gram-stain-negative, rod-shaped, indole-, oxidase- and catalase-negative, non-spore-forming, non-motile bacterium (strain 131000547(T)) was isolated from the lungs of a cat with pneumonia. On basis 16S rRNA gene sequence analyses strain assigned to genus Streptobacillus 97.6% similarity type moniliformis 94.6% that hongkongensis. The clear differentiation 131000547(T) hongkongensis also supported by gyrB, groEL, recA nucleotide amino acid analysis. DNA-DNA hybridization...
Two Corynebacterium strains were isolated from lymph nodes of wild boars showing severe alterations caused by caseous lymphadenitis. The came different districts in southern Germany; one was found dead, the other had been shot. two obtained both positive for phospholipase D. Further analysis biochemical profiles did not allow unambiguous differentiation between ulcerans and pseudotuberculosis. Fourier-transformed infrared spectroscopy as well partial sequencing genes 16S rRNA RNA polymerase...
Botulism outbreaks due to commercial products are extremely rare in the European Union. Here we report on first international outbreak of foodborne botulism caused by salt-cured, dried roach ( Rutilus rutilus ). Between November and December 2016, an six type E cases from five unrelated households was documented Germany Spain. The involved persons Russian Kazakh backgrounds, all consumed unheated roach—a snack particularly favored Easter-European countries. implicated food batches had been...
The zoonotic bacterium Corynebacterium ulcerans may be pathogenic both in humans and animals: toxigenic strains can cause diphtheria or diphtheria-like disease via toxin, while producing the dermonecrotic exotoxin phospholipase D lead to caseous lymphadenitis primarily wild animals. Diphtheria toxin-positive have been isolated mainly from cattle, dogs cats. Here, we report a series of ten isolations group water rats (Hydromys chrysogaster) with ulcerative skin lesions, which were kept zoo....
ABSTRACT The genes for two different protocatechuate 3,4-dioxygenases (P34Os) were cloned from the 4-sulfocatechol-degrading bacterium Agrobacterium radiobacter strain S2 (DSMZ 5681). pcaH1G1 encoded a P34O (P34O-I) which oxidized but not 4-sulfocatechol. These part of protocatechuate-degradative operon strongly resembled isofunctional protocatechuate-degrading tumefaciens A348 described previously by D. Parke (FEMS Microbiol. Lett. 146:3–12, 1997). second (P34O-II), pcaH2G2 genes, was...
The 4-carboxymethylen-4-sulfo-but-2-en-olide (4-sulfomuconolactone) hydrolases from Hydrogenophaga intermedia strain S1 and Agrobacterium radiobacter S2 are part of a modified protocatechuate pathway responsible for the degradation 4-sulfocatechol. In both strains, hydrolase-encoding genes occur downstream those encoding enzymes that catalyze lactonization 3-sulfomuconate. deduced amino acid sequences 4-sulfomuconolactone demonstrated highest degree sequence identity to...
Abstract Yersinia ruckeri is the causative agent of enteric redmouth disease (ERM), which mainly affects salmonid fish. Isolates Y. from diseased fish were obtained over a 6‐year period eight farms in State Baden‐Württemberg, Southwest Germany. The strains characterized by biochemical methods and Fourier transform infrared spectroscopy (FT‐IR) combined with artificial neural network analysis. These complemented 16S rDNA sequencing for several isolates. set these included sorbitol‐positive,...
Hydrogenophaga intermedia strain S1 and Agrobacterium radiobacter S2 form a mixed bacterial culture which degrades sulfanilate (4-aminobenzenesulfonate) by novel variation of the β -ketoadipate pathway via 4-sulfocatechol 3-sulfomuconate. It was previously proposed that further metabolism 3-sulfomuconate is catalysed modified 3-carboxy- cis , -muconate-lactonizing enzymes (CMLEs) these ‘type 2’ were different from conventional CMLEs (‘type 1’) protocatechuate in their ability to convert...