A5028825105- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Food Safety and Hygiene
- Essential Oils and Antimicrobial Activity
- Microbial Inactivation Methods
- Biosensors and Analytical Detection
- Identification and Quantification in Food
- Spectroscopy and Chemometric Analyses
- Bacillus and Francisella bacterial research
- Bacterial Identification and Susceptibility Testing
- Bacteriophages and microbial interactions
- Enterobacteriaceae and Cronobacter Research
- Vibrio bacteria research studies
- Mycobacterium research and diagnosis
- Medical Device Sterilization and Disinfection
- Viral gastroenteritis research and epidemiology
- Yersinia bacterium, plague, ectoparasites research
- Microbial infections and disease research
- Antibiotic Resistance in Bacteria
- Bee Products Chemical Analysis
- Mollusks and Parasites Studies
- Antimicrobial agents and applications
- Microbial Metabolism and Applications
- Vector-borne infectious diseases
- Plant Pathogens and Fungal Diseases
Austrian Agency for Health and Food Safety
2015-2025
Gesundheit Österreich
2009-2018
Graz University of Technology
2004
ABSTRACT Whole-genome sequencing (WGS) has emerged today as an ultimate typing tool to characterize Listeria monocytogenes outbreaks. However, data analysis and interlaboratory comparability of WGS are still challenging for most public health laboratories. Therefore, we have developed evaluated a new L. scheme based on genome-wide gene-by-gene comparisons (core genome multilocus the sequence [cgMLST]) allow unique nomenclature. Initially, determined breadth population MLST with Bayesian...
A cluster of seven human cases listeriosis occurred in Austria and Germany between April 2011 July 2013. The Listeria monocytogenes serovar (SV) 1/2b isolates shared pulsed-field gel electrophoresis (PFGE) fluorescent amplified fragment length polymorphism (fAFLP) patterns indistinguishable from those five food producers. isolates, a control strain with different PFGE/fAFLP profile ten were subjected to whole genome sequencing (WGS) blinded fashion. gene-by-gene comparison (multilocus...
is causing listeriosis, a rare but severe foodborne infection. Listeriosis affects pregnant women, newborns, older adults, and immunocompromised individuals. Ready-to-eat (RTE) foods are the most common sources of transmission pathogen This study explored virulence factors antibiotic resistance in
ABSTRACT Listeria monocytogenes presents a significant concern for the food industry due to its ability persist in processing environment. One of factors contributing persistence is decreased sensitivity disinfectants. Our objective was assess diversity L. disinfectants by testing response 1,671 L . isolates quaternary ammonium compounds (QACs) and 414 peracetic acid (PAA) using broth microdilution growth curve analysis assays, respectively, categorize into sensitive tolerant. A high...
We previously reported an outbreak of listeriosis in Austria and Germany due to consumption 'Quargel' cheese. It comprised 14 cases (including five fatalities) infected by a serotype 1/2a Listeria monocytogenes (clone 1), with onset illness from June 2009 January 2010. A second strain L. 2) spread this product could be linked further 13 (two fatal), six (one fatal) one case the Czech Republic, disease December end February .
We report an outbreak of listeriosis in Austria and Germany due to the consumption Quargel cheese produced by Austrian manufacturer. At time writing this report, was known account for 14 cases 2009, including four with lethal outcome. On 23 January 2010, product voluntarily withdrawn from market.
Background and aimThe trend in reported case counts of invasive Listeria monocytogenes (Lm), a potentially severe food-borne disease, has been increasing since 2008. In 2015, 2,224 cases were the European Union/European Economic Area (EU/EEA). We aimed to validate microbiological epidemiological aspects an envisaged EU/EEA-wide surveillance system enhanced by routine whole genome sequencing (WGS). Methods: WGS core multilocus sequence typing (cgMLST) performed on isolates from 2,726 27...
Abstract Invasive listeriosis is a severe foodborne infection in humans and difficult to control. Listeriosis incidence increasing worldwide, but some countries have implemented molecular surveillance programs improve recognition management of outbreaks. In Germany, routine whole-genome sequencing, core genome multilocus sequence typing, single nucleotide polymorphism calling are used for subtyping Listeria monocytogenes isolates from cases suspected foods. During 2018–2019, an unusually...
Listeria monocytogenes (L. monocytogenes) is a ubiquitous organism that can easily enter the food chain. Infection with L. cause invasive listeriosis. Since 2014, in Austria, isolates from human and food/food-associated samples have been provided on mandatory basis by producers laboratories to National Reference Laboratory. 2017, undergo routinely whole genome sequencing (WGS) core Multilocus Sequence Typing (cgMLST) for cluster analyses. Aims of this study were characterize clusters 2017...
Invasive listeriosis, caused by Listeria (L.) monocytogenes, is a severe foodborne infection, especially for immunocompromised individuals. The aim of our investigation was the identification and analysis listeriosis outbreaks in Germany with smoked graved salmon products as most likely source infection using whole-genome sequencing (WGS) patient interviews. In national surveillance programme, WGS used subtyping core genome multi locus sequence typing (cgMLST) cluster detection L....
Multidrug-resistant (MDR) Mycobacterium tuberculosis poses a serious threat to the control of (TB) and constitutes an increasing public health problem. The availability rapid in vitro susceptibility tests is prerequisite for optimal patient treatment. Rifampicin resistance caused by diverse mutations rpoB gene established widely used surrogate marker MDR-TB. We high-resolution melting (HRM) curve analysis approach scan gene.A total 49 MDR-TB 19 fully susceptible non-MDR-TB isolates, as...
Whole genome sequencing (WGS) is currently becoming the method of choice for characterization Listeria monocytogenes isolates in national reference laboratories (NRLs). WGS superior with regards to accuracy, resolution and analysis speed comparison several other methods including serotyping, PCR, pulsed field gel electrophoresis (PFGE), multilocus sequence typing (MLST), variable number tandem repeat (MLVA), multivirulence-locus (MVLST), which have been used thus far bacterial (and are still...
ABSTRACT Nanopore sequencing has shown the potential to democratize genomic pathogen surveillance due its ease of use and low entry cost. However, recent genotyping studies showed discrepant results compared gold-standard short-read sequencing. Furthermore, although essential for widespread application, reproducibility nanopore-only remains largely unresolved. In our multicenter performance study involving five laboratories, four public health-relevant bacterial species were sequenced with...
Introduction Listeria monocytogenes is an ubiquitous foodborne pathogen that represents a serious threat to public health and the food industry. Methods In this study Whole Genome Sequencing (WGS) was used characterize 160 L. isolates obtained from 22,593 different sources in Montenegro during years 2014–2022. Results Isolates belonged 21 clonal complexes (CCs), 22 sequence types (STs) 73 core genome multilocus (cgMLST) revealing high diversity. The most prevalent STs were ST8 ( n = 29), ST9...
Background Listeria (L.) monocytogenes is primarily transmitted via contaminated food and can cause listeriosis, an infection often associated with sepsis meningitis in at-risk individuals. Accurate outbreak detection relies on whole genome sequencing (WGS) core multilocus sequence typing (cgMLST), which use allele thresholds to identify related strains. Methods This study investigated mutation rates L. , focusing isolates DNA repair deficiencies. Serial subcultivations were performed,...
Abstract Background Listeria ( L .) monocytogenes strains show a high diversity regarding stress tolerance and virulence potential. Genome studies have mainly focused on specific sequence types (STs) predominantly associated with either food or human listeriosis. This study the prevalent ST155, showing equal distribution among clinical isolates. We evaluated potential of 20 ST155 performed comparative genomic analysis 130 isolated from food, processing environments listeriosis cases in...
Community-acquired pneumonia due to Pseudomonas aeruginosa in previously healthy individuals is a rare disease that associated with high fatality. On 14 February 2010 49-year-old woman presented an emergency room signs and symptoms of pneumonia, 2 days after returning from spa holiday wellness hotel. Blood cultures respiratory specimens grew P. aeruginosa. Despite adequate antimicrobial therapy, the patient died septic multiorgan failure on day nine hospitalization. 26, water samples were...
Bacillus cereus foodborne intoxications and toxicoinfections are on a rise. Usually, symptoms self-limiting but occasionally hospitalization is necessary. Severe with the emetic toxin cereulide, which notably resistant heat acid during cooking, can cause acute liver failure encephalopathy. We here present case series of food poisonings in five immunocompetent adults after ingestion fried rice balls, were massively contaminated cereus. The patients developed broad clinical spectrum, ranging...
Abstract Listeria monocytogenes ( Lm ) is a ubiquitous bacterium that causes listeriosis, serious foodborne illness. In the nature-to-human transmission route, can prosper in various ecological niches. Soil and decaying organic matter are its primary reservoirs. Certain clonal complexes (CCs) over-represented food production represent challenge to safety. To gain new understanding of adaptation mechanisms food, genetic background strains found animals environment should be investigated...
This study characterized five Cronobacter spp. and six Salmonella strains that had been isolated from 155 samples of powdered infant formula (PIF) sold in Chile manufactured Mexico 2018-2020. Two sakazakii sequence type (ST) ST1 ST31 (serotypes O:1 O:2) one strain malonaticus ST60 (O:1) were identified. All identified as Typhimurium ST19 (serotype O:4) by average nucleotide identity, ribosomal multilocus typing (rMLST), core genome MLST (cgMLST). The C. isolates resistant to cephalothin,...
Whole-genome sequencing (WGS) has revolutionized surveillance of infectious diseases. Disease outbreaks can now be detected with high precision, and correct attribution infection sources been improved. Listeriosis, caused by the bacterium Listeria monocytogenes, is a foodborne disease case fatality rate large proportion outbreak-related cases. Timely recognition listeriosis precise allocation food are important to prevent further infections promote public health. We report WGS-based...