A5077051438- Listeria monocytogenes in Food Safety
- Microbial Inactivation Methods
- Essential Oils and Antimicrobial Activity
- Salmonella and Campylobacter epidemiology
- Identification and Quantification in Food
- Biosensors and Analytical Detection
- Antimicrobial Resistance in Staphylococcus
- Microbial Metabolism and Applications
- Food Safety and Hygiene
- Probiotics and Fermented Foods
- Vibrio bacteria research studies
- Streptococcal Infections and Treatments
- Molecular Biology Techniques and Applications
- Bacterial biofilms and quorum sensing
- Pharmaceutical and Antibiotic Environmental Impacts
- Agroforestry and silvopastoral systems
- Antibiotic Use and Resistance
- Clostridium difficile and Clostridium perfringens research
- Enterobacteriaceae and Cronobacter Research
- Invertebrate Immune Response Mechanisms
- Antimicrobial agents and applications
- Banana Cultivation and Research
- Edible Oils Quality and Analysis
- Pneumonia and Respiratory Infections
- Viral Infections and Vectors
Université de Yaoundé I
2025
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2015-2024
Paris-Est Sup
2019-2024
Lister Institute of Preventive Medicine
2023
Département Santé Animale
2023
Agence Nationale des Fréquences
2018-2022
Sorbonne Université
2016
Makerere University
2014
Ministry of Health
2012
European Union
2012
Data on community spread of methicillin-resistant coagulase-negative staphylococci (MR-CoNS) are scarce. We assessed their potential role as a reservoir staphylococcal cassette chromosome mec (SCCmec) IVa, the leading SCCmec subtype in community-acquired Staphylococcus aureus (CA-MRSA).Nasal carriage MR-CoNS was prospectively investigated 291 adults at hospital admission. were characterized by typing, long-range polymerase chain reaction (PCR) for IV, and multiple-locus variable-number...
At the end of 2009, six food poisoning outbreaks caused by staphylococci were reported in France. Soft cheese made from unpasteurized milk was found to be common source outbreaks. Staphylococcal enterotoxin type E identified and quantified using both official confirmatory methods European Union Reference Laboratory (EU-RL). To our knowledge, this is first report staphylococcal
Concerns about food contamination by Listeria monocytogenes are on the rise with increasing consumption of ready-to-eat foods. Biofilm production L. is presumed to be one ways that confer its increased resistance and persistence in chain. In this study, a collection isolates from foods processing environments representing persistent, prevalent, rarely detected genotypes was evaluated for biofilm forming capacities including adhesion sessile biomass under diverse environmental conditions. The...
We present the LiSEQ (Listeria SEQuencing) project, funded by European Food Safety Agency (EFSA) to compare Listeria monocytogenes isolates collected in Union from ready-to-eat foods, compartments along food chain (e.g. food-producing animals, food-processing environments) and humans. In this article, we report molecular characterization of a selection data set employing whole-genome sequencing analysis. an overview strain diversity observed different sampled sources, characterize based on...
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ABSTRACT Listeria monocytogenes is a ubiquitous bacterium that may cause the foodborne illness listeriosis. Only small amount of data about population genetic structure strains isolated from food available. This study aimed to provide an accurate view L. strain in France. From 1999 2014, 1,894 were at French National Reference Laboratory for and classified according five risk matrices defined by European Food Safety Authority (EFSA). A total 396 selected on basis different pulsed-field gel...
In this contribution, the antimicrobial susceptibility toward 11 antibiotics and four biocides of a panel 205 Listeria monocytogenes (Lm) strains isolated from different ecological niches (i.e., food, animals natural environment) was evaluated. The impact exposure to on antibiotic susceptibilities Lm also investigated. food exhibited overall lower (higher minimal inhibitory concentrations, MIC) for ammonium quaternary compounds (QACs) peracetic acid (PAC) than environments. Conversely,...
Listeria monocytogenes is an ubiquitous pathogenic bacterium, transmissible to humans through the consumption of contaminated food. The pork production sector has been hit hard by a series L. monocytogenes-related food poisoning outbreaks in France. An overview diversity strains circulating at all levels chain, from pig farming finished products, needed identify contamination routes and improve safety. Until now, no typing data available on isolated across entire chain. Here, we analyzed...
Abstract Background Listeria monocytogenes is the causative agent of listeriosis, a serious disease affecting both animals and humans. Here, multilocus sequence typing (MLST) was used to characterize genetic diversity strains isolated from natural environment animal clinical cases in Europe. The prevalence clonal complexes (CCs) obtained compared according ( i ) origin isolation – vs. ii form listeriosis rhombencephalitis abortion. To this aim, two datasets were constructed. dataset...
Staphylococcal food poisoning outbreaks (SFPOs) are frequently reported in France. However, most of them remain unconfirmed, highlighting a need for better characterization isolated strains. Here we analyzed the genetic diversity 112 Staphylococcus aureus strains from 76 distinct SFPOs that occurred France over last 30 years. We used recently developed multiple-locus variable-number tandem-repeat analysis (MLVA) protocol and compared this method with pulsed field gel electrophoresis (PFGE),...
Listeria (L.) monocytogenes is a foodborne pathogen that can cause disease, mainly in elderly, pregnant or immunocompromised persons through consumption of contaminated food, including pork products. It widespread the environment and also be found asymptomatic carrier animals, for example, different tissues pigs. To learn more about their nature, 16 spp. isolates tonsils intestinal content pigs 13 from slaughterhouse were characterized using next-generation sequencing (NGS). A wide...
Background: Listeriosis, a bacterial disease in humans and animals, is mostly caused by ingestion of Listeria monocytogenes via contaminated food and/or water, or zoonotic infection. Globally, listeriosis has general low incidence but high case fatality rate. Objective: The objective this study was to investigate the occurrence, antimicrobial profiles, genetic relatedness L.monocytogenes from raw meat dairy products (raw milk, cottage cheese, cream cake), collected capital five neighboring...
Host-specificity is an intrinsic feature of many bacterial pathogens, resulting from a long history co-adaptation between bacteria and their hosts. Alpha-proteobacteria belonging to the genus Bartonella infect erythrocytes wide range mammal orders, including rodents. In this study, we performed genetic analysis colonizing rodent community dominated by bank voles (Myodes glareolus) wood mice (Apodemus sylvaticus) in French suburban forest evaluate diversity, capacity recombine level host...
Nasal carriage of methicillin-resistant coagulase-negative staphylococci (MR-CoNS) is highly prevalent in community subjects, but its dynamic has been little investigated. swabbing was performed 2006 and 2008 154 Amerindians living isolated French Guiana. MR-CoNS strains were identified characterized by non-β-lactam susceptibility testing staphylococcal cassette chromosome mec element (SCCmec) typing, characterizing the associations ccr gene complex allotypes, for MR Staphylococcus...
Abstract Listeria monocytogenes ( Lm ) is a ubiquitous bacterium that causes listeriosis, serious foodborne illness. In the nature-to-human transmission route, can prosper in various ecological niches. Soil and decaying organic matter are its primary reservoirs. Certain clonal complexes (CCs) over-represented food production represent challenge to safety. To gain new understanding of adaptation mechanisms food, genetic background strains found animals environment should be investigated...
Listeriosis is a severe infection which mainly affects pregnant women, neonates and immuno-compromised adults. ANSES’s Laboratory for Food safety has been the European Union Reference (EURL) L. monocytogenes in food chain since 2006. Pulsed Field Gel Electrophoresis (PFGE) routinely used EURL surveillance of isolated from foods, animals environment. One main activities to evaluate alternative molecular subtyping methods PFGE, integrate their use within National Laboratories (NRL) network....
Listeria monocytogenes is a ubiquitous bacterium that causes foodborne illness, listeriosis. Most strains can be classified into major clonal complexes (CCs) account for the majority of outbreaks and sporadic cases in Europe. In addition to 20 CCs known human animal clinical cases, 10 are frequently reported food production, thereby posing serious challenge agrifood industry. Therefore, there need rapid reliable method identify these 30 CCs. The high-throughput real-time PCR assay presented...
The European Union Reference Laboratory for Listeria monocytogenes (EURL L. monocytogenes) coordinates a network of 29 National Laboratories (NRLs). Depending on national decision, NRLs undertake food, environmental, and veterinary strain surveillance in their respective countries. In the framework PulseNet Europe network, two pulsed-field gel electrophoresis (PFGE) subtyping proficiency testing (PT) trials were carried out 2003 2006. obtained data showed that PFGE profiles can be compared...
Over the past 11 years, Slovak National Reference Laboratory has collected a panel of 988 Listeria monocytogenes isolates in Slovakia, which were isolated from various food sectors (61%), food-processing environments (13.7%), animals with listeriosis symptoms (21.2%), and human cases (4.1%). We serotyped these by agglutination method, revealed highest prevalence (61.1%) serotype 1/2a lowest (4.7%) 1/2c, although represented majority meat sector. The distribution CCs analyzed on 176...