A5102712406- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Botulinum Toxin and Related Neurological Disorders
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Streptococcal Infections and Treatments
- Milk Quality and Mastitis in Dairy Cows
- RNA and protein synthesis mechanisms
- Stroke Rehabilitation and Recovery
- Bacterial Identification and Susceptibility Testing
- Microbial Metabolism and Applications
- Bacterial Genetics and Biotechnology
- Probiotics and Fermented Foods
- Bioinformatics and Genomic Networks
- Healthcare Systems and Technology
- Tendon Structure and Treatment
- Advanced Proteomics Techniques and Applications
- Gene expression and cancer classification
- Hereditary Neurological Disorders
- Shoulder and Clavicle Injuries
- Oral microbiology and periodontitis research
- Shoulder Injury and Treatment
- Musculoskeletal pain and rehabilitation
- Machine Learning in Bioinformatics
- Biochemical and Structural Characterization
Hospital Universitario La Paz
1999-2025
Foundation for Physical Medicine and Rehabilitation
2023
Universidad Autónoma de Madrid
2021-2023
Hospital La Paz Institute for Health Research
2021-2023
University of Rochester Medical Center
2022
Universidad Rey Juan Carlos
2019-2021
Sociedad Española de Cirugía Ortopédica y Traumatología
2020
National University of the Northeast
2020
University of Geneva
2006-2014
Scripps Research Institute
2013
Novel high-throughput DNA sequencing technologies allow researchers to characterize a bacterial genome during single experiment and at moderate cost. However, the increase in throughput that is allowed by using such platforms obtained expense of individual sequence read length, which must be assembled into longer contigs exploitable. This study focuses on Illumina platform produces millions very short sequences are 35 bases length. We propose de novo assembler software dedicated process...
Translation of the stationary phase sigma factor RpoS is stimulated by at least two small RNAs, DsrA and RprA. disrupts an inhibitory secondary structure in rpoS leader mRNA pairing with upstream RNA. Mutations rprA compensating mutations demonstrate that RprA interacts same region as DsrA. This first example different RNAs regulating a common target. Regulation these differs. synthesis increased low temperature. We find regulated RcsC/RcsB phosphorelay system, previously found to regulate...
Staphylococcus aureus RN4220, a cloning intermediate, is sometimes used in virulence, resistance, and metabolic studies. Using whole-genome sequencing, we showed that RN4220 differs from NCTC8325 contains number of genetic polymorphisms affect both virulence general fitness, implying need for caution using this strain such
Data on community spread of methicillin-resistant coagulase-negative staphylococci (MR-CoNS) are scarce. We assessed their potential role as a reservoir staphylococcal cassette chromosome mec (SCCmec) IVa, the leading SCCmec subtype in community-acquired Staphylococcus aureus (CA-MRSA).Nasal carriage MR-CoNS was prospectively investigated 291 adults at hospital admission. were characterized by typing, long-range polymerase chain reaction (PCR) for IV, and multiple-locus variable-number...
Staphylococcus aureus is a major human pathogen responsible for variety of nosocomial and community-acquired infections. Recent reports show that the prevalence Methicillin-Resistant S. (MRSA) infections in cystic fibrosis (CF) patients increasing. In 2006 Marseille, France, we have detected an atypical MRSA strain with specific antibiotic susceptibility profile unique growth phenotype. Because clinical importance spread such among CF decided to sequence genome one representative isolate...
Background Staphylococcus aureus is a versatile bacterial opportunist responsible for wide spectrum of infections. The severity these infections highly variable and depends on multiple parameters including the genome content bacterium as well condition infected host. Clinically epidemiologically, S. shows particular capacity to survive adapt drastic environmental changes presence numerous antimicrobial agents. Mechanisms triggering this adaptation remain largely unknown despite important...
Staphylococcus aureus is an versatile pathogen that can cause life-threatening infections. Depending on the clinical setting, up to 50% of S. infections are caused by methicillin-resistant strains (MRSA) in most cases resistant many other antibiotics, making treatment difficult. The emergence community-acquired MRSA drastically changed picture increasing risk Horizontal transfer genes encoding for antibiotic resistance or virulence factors a major concern multidrug-resistant and...
BackgroundThe arginine catabolic mobile element (ACME) associated with staphylococcal cassette chromosome mec (SCCmec) in the USA300 clone of community-acquired methicillin-resistant Staphylococcus aureus enhances its fitness and ability to colonize host. epidermidis may act as a reservoir ACME for S. aureus. We assessed diffusion (MRSE) isolates colonizing outpatients.
ABSTRACT The critical role of noncoding small RNAs (sRNAs) in the bacterial response to changing conditions is increasingly recognized. However, a specific for sRNAs during antibiotic exposure has not been investigated Staphylococcus aureus . Here, we used Illumina RNA-Seq examine sRNA multiresistant sequence type 239 (ST239) S. after four antibiotics (vancomycin, linezolid, ceftobiprole, and tigecycline) representing major classes antimicrobials treat methicillin-resistant (MRSA)...
S. aureus is one of the main pathogens involved in ruminant mastitis worldwide. The severity staphylococcal infection highly variable, ranging from subclinical to gangrenous mastitis. This work represents an in-depth characterization isolates identify bacterial factors infection.
Staphylococcus aureus is responsible for a broad variety of chronic infections. Most S. clinical isolates show the capacity to adhere abiotic surfaces and develop biofilms. Because growing in biofilm highly refractory treatment, inhibition formation represents major therapeutic objective. We evaluated effects oleic acid on primary adhesion production eight genotypically different strains as well biofilm-negative carnosus strain TM300. Oleic inhibited but increased every tested. UAMS-1 was...
The salivary microbiota is a potential diagnostic indicator of several diseases. Culture-independent techniques are required to study the microbial community since many its members have not been cultivated.We explored bacterial composition in saliva sample using metagenomic whole genome shotgun (WGS) sequencing, extraction 16S rRNA gene fragments from sequences (16S-WGS) and high-throughput sequencing PCR-amplified rDNA (16S-HTS) regions V1 V3.The hierarchical clustering data based on...
Introduction Current care plans for stroke survivors typically focus on acute management, resulting in many being discharged to their communities without adequate follow-up, despite often experiencing significant post-stroke complications, such as spasticity (PSS). While studies have explored the incidence and prevalence of PSS, little is known about how early PSS develops develop ‘problematic’ that would benefit from pharmacological treatment. Methods analysis EPITOME a prospective,...
Abstract Motivation: Paired-end sequencing allows circumventing the shortness of reads produced by second generation sequencers and is essential for de novo assembly genomes. However, obtaining a finished genome from short still an open challenge. We present algorithm that exploits pairing information issued inserts potentially any length. The method determines paths through overlaps graph using constrained search tree. also automatically suited cutoffs according to contextual coverage,...
Staphylococcus aureus is a major cause of mastitis in ruminants. In ewe mastitis, symptoms range from subclinical to gangrenous mastitis. S. factors or host-factors contributing the different outcomes are not completely elucidated. this study, experimental was induced on primiparous ewes using two strains, isolated (strain O11) O46) Strains drastically distinct clinical when tested and mice Notably, they reproduced mild (O46) severe (O11) ewes. Ewe sera were used identify staphylococcal...
ABSTRACT Staphylococcus aureus is a major etiological agent of mastitis in ruminants. We report here the genome sequences two ovine strains that were isolated from gangrenous (strain O11) and subclinical O46) ewe mastitis. Both belong to same clonal complex. Despite this close genotypic relationship, isolates shown reproducibly induce highly divergent types infections, either severe (O11) or mild (O46) mastitis, an experimental model.
S. aureus is a major aetiological agent of ruminant mastitis worldwide. The chronic nature makes it difficult to cure and prone resurgence. In order identify the bacterial factors involved in this chronicity, Newbould 305 (N305), strain that can reproducibly induce mild an experimental setting, was characterized depth. We employed genomic proteomic techniques combined with phenotype characterization, comprehensively analyse N305. results were compared data obtained on RF122, representative...
ABSTRACT Small-colony variants (SCVs) of bacteria are associated with recurrent and persistent infections. We describe for the first time SCVs Streptococcus tigurinus in a patient prosthetic joint infection. S. is novel pathogen mitis group causes invasive sought to characterize using experimental methods find possible genetic explanations their phenotypes. The were compared wild-type (WT) isolate phenotypic methods, including growth under different conditions, autolysis, visualization cell...
C1A cysteine peptidases are synthesized as inactive proenzymes. Activation takes place by proteolysis cleaving off the inhibitory propeptide. The capacity of propeptides from barley cathepsin L and B-like towards commercial cathepsins has been characterized. Differences in selectivity have found for L-cathepsins against their cognate non enzymes. Besides, propeptide B was not able to inhibit bovine B. Modelling three-dimensional structures suggests that most properties can be explained...