A5075612264- Listeria monocytogenes in Food Safety
- Identification and Quantification in Food
- Salmonella and Campylobacter epidemiology
- Microbial Inactivation Methods
- Essential Oils and Antimicrobial Activity
- Food Safety and Hygiene
- Spectroscopy and Chemometric Analyses
- Vibrio bacteria research studies
- Biosensors and Analytical Detection
- Molecular Biology Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Aquaculture Nutrition and Growth
- Medical Device Sterilization and Disinfection
- Viral Infectious Diseases and Gene Expression in Insects
- Genomics and Phylogenetic Studies
- Plant Pathogenic Bacteria Studies
- Escherichia coli research studies
- Animal Nutrition and Physiology
- Insect behavior and control techniques
- Antimicrobial agents and applications
- Aquaculture disease management and microbiota
- Postharvest Quality and Shelf Life Management
- Bacteriophages and microbial interactions
- Fish Ecology and Management Studies
- Microbial infections and disease research
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2015-2025
ABSTRACT Listeria monocytogenes presents a significant concern for the food industry due to its ability persist in processing environment. One of factors contributing persistence is decreased sensitivity disinfectants. Our objective was assess diversity L. disinfectants by testing response 1,671 L . isolates quaternary ammonium compounds (QACs) and 414 peracetic acid (PAA) using broth microdilution growth curve analysis assays, respectively, categorize into sensitive tolerant. A high...
Listeria monocytogenes is an important foodborne pathogen, markedly persistent even in harsh environments and responsible for high hospitalization mortality rates. The aim of the present study was to detect strains circulating Sicily over a five-year period characterize their antimicrobial resistance profiles. key element this sharing data among various entities involved food control clinical surveillance listeriosis order develop integrated approach pathogen. A total 128 isolates were...
Purpose. From May 2015 to March 2016, an outbreak due Listeria monocytogenes serotype 1/2a and clinical pulsotype never previously isolated in Europe occurred central Italy, involving 24 confirmed cases. The article provides a description of the investigation carried out by multidisciplinary network. Methodology. Epidemiological microbiological surveillance was conducted confirm detect food vehicle infection. origin destination implicated its ingredients were investigated tracing-back...
A total of 66 Listeria monocytogenes (Lm) isolated from 2013 to 2018 in a small-scale meat processing plant and dairy facility Central Italy were studied. Whole Genome Sequencing bioinformatics analysis used assess the genetic relationships between strains investigate persistence virulence abilities. The biofilm forming-ability was assessed vitro. Cluster grouped Lm into three main clusters: two them, both belonging CC9, persisted for years one (CC121) last year sampling. In facility, all...
Abstract Listeria monocytogenes ( Lm ) is a ubiquitous bacterium that causes listeriosis, serious foodborne illness. In the nature-to-human transmission route, can prosper in various ecological niches. Soil and decaying organic matter are its primary reservoirs. Certain clonal complexes (CCs) over-represented food production represent challenge to safety. To gain new understanding of adaptation mechanisms food, genetic background strains found animals environment should be investigated...
Ready-to-eat (RTE) vegetables, including fresh-cut ones, are exposed to the risk of foodborne pathogen Listeria monocytogenes. Nowadays, products widely consumed because their convenience. Nevertheless, effective washing treatments required for preparation, maintain freshness and microbiological safety. Currently, natural substances increasingly demanded as alternatives traditional synthetic agents. Therefore, this study investigates application Thymbra capitata hydrolate solution rocket...
In 2012 a US multistate outbreak of listeriosis was linked to ricotta salata imported from Italy, made pasteurized sheep's milk. Sampling activities were conducted in Italy trace the source Listeria monocytogenes contamination. The cheese that caused produced plant Apulia processed semi-finished cheeses supplied by five plants Sardinia. During an 'emergency sampling', 179 (23·6%) out 758 end-products tested positive for L. monocytogenes, with concentrations <10 c.f.u./g 1·1 × 106 c.f.u./g....
Whole genome sequencing analyzed by core multi-locus sequence typing (cgMLST) is widely used in surveillance of the pathogenic bacteria Listeria monocytogenes. Given heterogeneity available bioinformatics tools to define cgMLST alleles, our aim was identify parameters influencing precision profiles.We three L. monocytogenes reference genomes from different phylogenetic lineages and assessed impact vitro (i.e. tested genomes, successive platings, replicates DNA extraction sequencing) silico...
Listeria monocytogenes is a ubiquitous bacterium that causes foodborne illness, listeriosis. Most strains can be classified into major clonal complexes (CCs) account for the majority of outbreaks and sporadic cases in Europe. In addition to 20 CCs known human animal clinical cases, 10 are frequently reported food production, thereby posing serious challenge agrifood industry. Therefore, there need rapid reliable method identify these 30 CCs. The high-throughput real-time PCR assay presented...
Listeria monocytogenes (Lm) can persist in food processing environments (FPEs), surviving environmental stresses and disinfectants. We described an intensive monitoring plan performed Central Italy involving producing plants (FPPs) retail grocery stores (RSs). The aim of the study was to provide a snapshot Lm circulation different FPEs during severe listeriosis outbreak, using whole genome sequencing (WGS) investigate genetic diversity isolated, evaluating their virulence stress resistance...
Human listeriosis outbreaks are often associated with food products, which could be contaminated, at the same time, also by different clones of Listeria monocytogenes. This emphasize need to type more than one L.monocytogenes isolate found in a single or environmental sample. The purpose present study was evaluate presence strains and production environment order understand if there is isolates from sample case L.monocytogenes. Between 2011 2015, Italian National Reference Laboratory for...
The contamination of ready to eat foods (RTE) products due Listeria monocytogenes could compromise the safety becoming a great risk for consumers. high presence L. in RTE has been described worldwide, but few data are available about these from African countries. aims this study were report Zambian products, providing genomic characterization and on similarity with circulating strains using whole genome sequencing (WGS). A total 304 produced by different manufacturers, purchased at retail,...
Although there are increasing reports on the prevalence of Listeria monocytogenes in wild species, this is first case listeriosis sea turtle. An adult female Caretta caretta was rescued after being stranded alive along coast Abruzzo region (Italy) summer 2021. The turtle died 6 days due to respiratory failure. necropsy showed widespread organ lesions, such as yellow foci necrosis many organs, gastrointestinal erosions, pericarditis, and granulomatous pneumonia. Microbiological histological...
Listeria monocytogenes (Lm) is a ubiquitous bacterium that causes the foodborne illness, listeriosis. Clonal complexes (CC), such as CC121, are overrepresented in food production industry, and rarely reported animals environment. Working within European-wide project, we investigated routes by which strains transmitted from environments to environment (FPE). In this context, report, for first time, occurrence of ST121 (CC121) strain isolated dolphin brain. The genome was compared with genomes...
From January 2015 to March 2016, an outbreak of 23 human cases listeriosis in the Marche region and one case Umbria Italy was caused by Listeria monocytogenes strains showing a new pulsotype never described before Italy. A total 37 clinical isolated from patients exhibiting symptoms 1374 correlated were received Italian National Reference Laboratory for L. (It NRL Lm) Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise (IZSAM) investigation. real-time PCR assay purposely designed...
is a significant concern in the food industry due to its association with outbreaks of listeriosis, particularly affecting vulnerable populations. High-throughput technologies such as RNA sequencing (RNA-seq) and proteomics offer valuable insights into molecular responses
The present study evaluated the presence of Listeria spp. and L. monocytogenes in four plants producing PDO Taleggio cheese. A total 360 environmental samples were collected from different areas during production. sampling points identified as Food Contact Surfaces (FCS), transfer-Non (tr-NFCS), non-transfer-NFCS (non-tr-NFCS). Fifty-nine ingredients/products also analyzed. was found all with a mean prevalence 23.1%; that included ripening area showed significantly higher if compared to...
From May 2015 to March 2016, a severe outbreak due Listeria monocytogenes ST7 strain occurred in Central Italy and caused 24 confirmed clinical cases. The epidemic was deeply investigated using whole-genome sequencing (WGS) analysis. In the interested area, foodborne investigation identified meat food-producing plant contaminated by strain, carried pork-ready-to-eat products. same region, 2018, reemerged causing one listeriosis case 10-month-old child. aim of this study investigate phylogeny...