A5042175948- Brucella: diagnosis, epidemiology, treatment
- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- Vector-Borne Animal Diseases
- Identification and Quantification in Food
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Trypanosoma species research and implications
- Escherichia coli research studies
- Advanced Biosensing Techniques and Applications
- Microbial infections and disease research
- Toxin Mechanisms and Immunotoxins
- Viral Infections and Vectors
- Parasitic Infections and Diagnostics
- vaccines and immunoinformatics approaches
- Hereditary Neurological Disorders
- Peripheral Neuropathies and Disorders
- Mycobacterium research and diagnosis
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Inactivation Methods
- Burkholderia infections and melioidosis
- bioluminescence and chemiluminescence research
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2015-2024
University of Teramo
2012-2024
Istituto Zooprofilattico Sperimentale delle Venezie
2024
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"
2016
Istituto Giordano
2012
University of Bologna
2007
A simple and rapid multiplexed sandwich chemiluminescent enzyme immunoassay has been developed for the simultaneous detection of Escherichia coli O157:H7, Yersinia enterocolitica, Salmonella typhimurium, Listeria monocytogenes. To achieve four pathogens, a new polystyrene 96 well microtiter plate format designed, in which each main contains subwells bottom. The monoclonal antibodies specific bacteria were separately immobilized subwell. When samples added to wells, able specifically bind...
There is strong evidence that severe acute respiratory syndrome 2 virus (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19) pandemic, originated from an animal reservoir. However, exact mechanisms emergence, host species involved, and risk to domestic agricultural animals are largely unknown. Some species, including cats, ferrets, minks, have been demonstrated be susceptible SARS-CoV-2 infection, while others, such as pigs chickens, not. Importantly, susceptibility...
Abstract Background Contagious Bovine Pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. , is widespread in sub-Saharan Africa. The current live vaccine T1/44 has limited efficacy and occasionally leads to severe side effects the animals. A better understanding of immune responses triggered their role disease progression will help facilitate design a rational vaccine. Currently, knowledge cytokines involved immunity immunopathology CBPP rather limited. aim this study was...
Canine brucellosis caused by Brucella canis, is an infectious disease affecting dogs and wild Canidae. Clinical diagnosis challenging, laboratory testing crucial for a definitive diagnosis. Various serological methods have been described, but their accuracy uncertain due to limited validation studies. The present study aimed evaluate the performances of three tests B. canis in comparison with bacterial isolation (gold standard), order establish protocol canine brucellosis. A panel sera from...
Summary A competitive ELISA ( Brucella ‐Ab c‐ELISA) was standardized and validated for the detection of antibodies in cattle, sheep goat sera using a monoclonal antibody (MAb 4B5A) produced against melitensis biotype 2. The specificity sensitivity assay were 100% to 67.5% cut‐off point (B/Bo%). When compared with an indirect ELISA, c‐ELISA did not demonstrate cross‐reactions when testing positive some Enterobacteriaceae. comparison made between complement fixation Rose Bengal tests. Results...
Monoclonal antibodies (MAbs) specific for the lipopolysaccharide (LPS) of Escherichia coli O104:H4 were produced by fusion Sp2/O-Ag-14 mouse myeloma cells with spleen Balb/c mice, immunized heat-inactivated and sonicated E. bacterial cells. Four MAbs LPS (1E6G6, 1F4C9, 3G6G7 4G10D2) characterized evaluated use in a method detection milk samples that involves antibody conjugation to magnetic microbeads reduce time increase efficiency isolation. MAb 1E6G6 was selected coupled microbeads, then...
In the Schwann cells and neuronal plasma membranes gangliosides are organized in clusters forming complexes of microdomains termed lipid rafts. We investigated frequency, clinical correlates, fine specificity pro-inflammatory properties antibodies to ganglioside (GSCs) a Guillain Barré syndrome (GBS) population. 63 patients with different GBS variants we performed an ELISA for Campylobacter Jejuni ( C. jejuni), GSCs. studied GSCs by immunoabsorption study complement activation assay....
The diagnosis of dourine can be difficult because the clinical signs this disease in horses are similar to those surra, caused by Trypanosoma evansi. Moreover, T. equiperdum and evansi closely related and, so far, they cannot distinguished using serological tests. In a previous work, protein pattern recognized antibodies from dourine-infected humoral immune response kinetics were investigated immunoblotting assay; total 20 sera naturally experimentally infected healthy animals tested....
The significance of Trypanosoma equiperdum as the causative agent dourine cannot be understated, especially given its high mortality rate among equids. International movement equids should subject to thorough health checks and screenings ensure that animals are not infected with equiperdum. This involves implementation quarantine protocols, testing procedures, issuance certificates certify status animals. Three proteins, peptidyl-prolyl cis-trans isomerase (A0A1G4I8N3), GrpE protein homolog...
is a significant concern in the food industry due to its association with outbreaks of listeriosis, particularly affecting vulnerable populations. High-throughput technologies such as RNA sequencing (RNA-seq) and proteomics offer valuable insights into molecular responses
Up to 80 million cases of food-borne illness occur yearly in the US. Approximately 30% these are caused by bacteria and their related toxic products (1)(2). The species that most frequently responsible for food contaminations Salmonella typhimurium (meat, milk, eggs), Escherichia coli O157:H7 (meat), Staphylococcus aureus (milk, cream, meat), Clostridium perfringens (sausages, preserved food), Campylobacter jejuni (poultry, Vibrio parahaemolyticus (shellfish), Yersinia enterocolitica milk),...
Four hybridoma cell lines (C4B, 10C2G5, 6C5F4C7, 2D10G11) were adapted to grow in serum-free conditions. Cell proliferation, viability, and antibody production Nutridoma SP Ex-Cell HSF 610 media evaluated results compared with those obtained using DMEM containing 10% fetal bovine serum (control medium). Clone C4B showed the best IgG productivity control medium, but viability number of cells per milliliter similar for three media. For clone highest values both medium SP; no significant...
Listeria monocytogenes is a foodborne pathogen that ubiquitous and largely distributed in food manufacturing environments. It responsible for listeriosis, disease can lead to significant morbidity fatality immunocompromised patients, pregnant women, newborns. Few reports have been published about proteome adaptation when L. cultivated stress conditions. In this study, we applied one-dimensional electrophoresis 2D-PAGE combined with tandem mass spectrometry evaluate profiling the following...
Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides var. small colony (MmmSC), is one of the most important diseases cattle in Africa. The role innate or acquired cell mediated and humoral immunity conferring protection against MmmSC infection has not yet been elucidated. On other hand, pathological lesions aetiological agent have considered indicative an immunopathological process. In this study ten naïve were exposed to in-contact with animals infected intubation a...
Listeria monocytogenes is one of the main foodborne pathogens worldwide. Although its response to stress conditions has been extensively studied, it still present in food processing environments and a concern for consumers. To investigate how this microorganism adapts proteome mild conditions, combined proteomics bioinformatics approach was used characterize immunogenic protein profile sequence type 7 (ST7) strain that caused severe listeriosis outbreaks central Italy. Extracted proteins...