A5053482376- Analytical Chemistry and Sensors
- Conducting polymers and applications
- bioluminescence and chemiluminescence research
- Biosensors and Analytical Detection
- Molecular Junctions and Nanostructures
- Organic Electronics and Photovoltaics
- Advanced Biosensing Techniques and Applications
- Electrochemical sensors and biosensors
- Neuroscience and Neural Engineering
- Nanowire Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Advanced Sensor and Energy Harvesting Materials
- Force Microscopy Techniques and Applications
- Electrochemical Analysis and Applications
- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Photoreceptor and optogenetics research
- Thin-Film Transistor Technologies
- Bacterial Identification and Susceptibility Testing
- Advanced Chemical Sensor Technologies
- Mechanical and Optical Resonators
- Mycotoxins in Agriculture and Food
- Identification and Quantification in Food
- 3D Printing in Biomedical Research
- Gastroesophageal reflux and treatments
University of Bari Aldo Moro
2010-2018
University of Florence
2014
University of Bologna
2005-2013
Istituto Nazionale Biostrutture e Biosistemi
2013
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2005-2007
Pharmaceutical Biotechnology (Czechia)
2005
Abstract Peripheral events in olfaction involve odorant binding proteins (OBPs) whose role the recognition of different volatile chemicals is yet unclear. Here we report on sensitive and quantitative measurement weak interactions associated with neutral enantiomers differentially to OBPs immobilized through a self-assembled monolayer gate an organic bio-electronic transistor. The transduction remarkably as transistor output current governed by small capacitance protein layer undergoing...
Electrolyte-gated organic field-effect transistors are successfully used as biosensors to detect binding events occurring at distances from the transistor electronic channel that much larger than Debye length in highly concentrated solutions. The sensing mechanism is mainly capacitive and due formation of Donnan's equilibria within protein layer, leading an extra capacitance (C DON) series gating system. As a service our authors readers, this journal provides supporting information supplied...
Anchored, biotinylated phospholipids forming the capturing layers in an electrolyte-gated organic field-effect transistor (EGOFET) allow label-free electronic specific detection at a concentration level of 10 nM high ionic strength solution. The sensing mechanism is based on clear capacitive effect across PL involving charges target molecules.
A simple and rapid multiplexed sandwich chemiluminescent enzyme immunoassay has been developed for the simultaneous detection of Escherichia coli O157:H7, Yersinia enterocolitica, Salmonella typhimurium, Listeria monocytogenes. To achieve four pathogens, a new polystyrene 96 well microtiter plate format designed, in which each main contains subwells bottom. The monoclonal antibodies specific bacteria were separately immobilized subwell. When samples added to wells, able specifically bind...
Bio-sensing represents one of the most attractive applications carbon material based electronic devices; nevertheless, complete integration bioactive transducing elements still a major challenge, particularly in terms preserving biological function and specificity while maintaining sensor's performance. This review highlights recent advances realization field-effect transistor (FET) sensors that comprise bio-receptor within FET channel. A birds-eye view will be provided promising classes...
The detection of protein biomarkers is great importance in the early diagnosis severe pathological states. Although last decade many approaches to achieve ultra-sensitive have been developed, most them require complicated assay set-ups, hindering their adoption point-of-care applications and on-spot diagnosis. Here we show an organic electrochemical transistor (OECT) biosensor printed on plastic substrates that can selectively detect Immunoglobulin G (IgG) with unprecedented attomolar limit....
Biosystems integration into an organic field-effect transistor (OFET) structure is achieved by spin coating phospholipid or protein layers between the gate dielectric and semiconductor. An architecture directly interfacing supported biological to OFET channel proposed and, strikingly, both electronic properties biointerlayer functionality are fully retained. The platform bench tests involved OFETs integrating phospholipids bacteriorhodopsin exposed 1-5% anesthetic doses that reveal...
This review discusses the state-of-the-art strategies for realizing TFTs by printing compatible techniques, focusing attention on label-free electronic biosensors.
A fast and ultrasensitive chemiluminescent enzyme immunoassay for aflatoxin M1 in milk samples has been developed validated. The method is an indirect competitive type format involving the immobilization of M1−bovine serum albumin conjugate on 384 well black polystyrene microtiter plates use a secondary antibody labeled with horseradish peroxidase detected luminol-based substrate. Aflatoxin standard solutions were prepared milk-based buffer, analyzed without any cleanup procedure. limit...
A Functional Bio-Interlayer Organic Field-Effect Transistor (FBI-OFET) sensor, embedding a streptavidin protein capturing layer, capable of performing label-free selective electronic detection biotin at 3 part per trillion (mass fraction) or 15 pM, is proposed here. The response shows logarithmic dependence spanning over 5 orders magnitude analyte concentration. optimization the FBI analytical performances achieved by depositing layer through controllable Layer-by-Layer (LbL) assembly, while...
A sensitive EGOFET biosensor fabricated by anchoring bioprobes through a UV crosslinked poly(acrylic acid) coating deposited on an organic semiconductor.
Bottom- and top-contact organic thin film transistors (OTFTs) were fabricated, using poly(3-hexylthiophene-2,5-diyl) (P3HT) poly[2,5-bis(3-tetradecylthiophen-2-yl)thieno[3,2-b]thiophene] (PBTTT-C16) as p-type channel semiconductors. Four different types of OTFTs fabricated investigated gas sensors against three volatile compounds, with associated dipole moments. The OTFT-based sensor responses evaluated static transient current measurements. A comparison between the architectures relative...
A jellified alginate based capsule serves as biocompatible and biodegradable electrolyte system to gate an organic field-effect transistor fabricated on a flexible substrate. Such allows operating thiophene polymer transistors below 0.5 V through electrical double layer formed across ion-permeable polymeric electrolyte. Moreover, biological macro-molecules such glucose-oxidase streptavidin can enter into the gating capsules that serve also delivery system. An enzymatic bio-reaction is shown...
We report on the use of a polyanionic proton conductor, poly(acrylic acid), to gate poly[2,5-bis(3-tetradecylthiophen-2-yl)thieno[3,2-b]thiophene]-based organic field-effect transistor (OFET). A planar configuration OFET is evaluated, and electrical performance implementation flexible substrate are discussed.
Among the metal oxide semiconductors, ZnO has been widely investigated as a channel material in thin-film transistors (TFTs) due to its excellent electrical properties, optical transparency and simple fabrication via solution-processed techniques. Herein, we report solution-processable ZnO-based transistor gated through liquid electrolyte with an ionic strength comparable that of physiological fluid. The surface morphology chemical composition films upon exposure water phosphate-buffered...
Abstract The study of proteins confined on a surface has attracted great deal attention due to its relevance in the development bio-systems for laboratory and clinical settings. In this respect, organic bio-electronic platforms can be used as tools achieve deeper understanding processes involving protein interfaces. work, biotin-binding have been integrated two different thin-film transistor (TFT) configurations separately address changes occurring protein-ligand complex morphology dipole...
Abstract A plasma deposition (PE‐CVD) process is proposed to functionalize the surface of P3HT organic semiconductor with an hydrophilic coating characterized by carboxyl groups. The functionalized employed as electronic active layer in electrolyte gated field‐effect transistor (EGOFET). Nanometric coatings were deposited at from glow discharges fed ethylene and acrylic acid vapors. chemical composition coated along its wettability, have been assessed means X‐ray photoelectron spectroscopy...
Purpose Acute liver failure (ALF) and acute on chronic (ACLF) still show a poor prognosis. MARS was used in 22 patients with ALF or ACLF to prolong patient survival for function recovery as bridge transplantation. Design Evaluation of depurative efficiency, biocompatibility, hemodynamics, encephalopathy (HE) clinical outcome. Procedures During 71 five-hour sessions we evaluated (0′, 60′, 120′, 180′, 240′, 300′): bilirubin, ammonia, cholic acid (CCA), chenodeoxycholic (CCDCA), leukocytes,...