A5024193421- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Vector-borne infectious diseases
- Lepidoptera: Biology and Taxonomy
- Mosquito-borne diseases and control
- Freshwater macroinvertebrate diversity and ecology
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Identification and Quantification in Food
- Viral gastroenteritis research and epidemiology
- Fossil Insects in Amber
- Food Safety and Hygiene
- Microbial infections and disease research
- Microbial Inactivation Methods
- Virus-based gene therapy research
- Insect and Pesticide Research
- Malaria Research and Control
- Insect symbiosis and bacterial influences
- Botany and Plant Ecology Studies
- Yersinia bacterium, plague, ectoparasites research
- SARS-CoV-2 and COVID-19 Research
- Morphological variations and asymmetry
- Ecology and Vegetation Dynamics Studies
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2016-2025
University of Teramo
2023
Azienda USL di Pescara
2023
University of Siena
2016-2021
Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana
2019
Ospedale Maggiore
2006
University of Rome Tor Vergata
2000-2005
Ospedale Bellaria
2002-2003
Following the announcement on December 2020 about emergence of a new variant (VOC 202012/ 01, B.1.1.7 lineage) in United Kingdom, targeted surveillance was put place Abruzzo region (Italy), which allowed detection 313 persons affected by lineage B.1.1.7, up to 20th February 2021. We investigated results RT-PCR nasopharyngeal swabs tested from 2021 verify any difference viral load and persistence between people infected others. Statistically significant lower values CT associated with N...
Biting midges (Culicoides spp.) are important vectors of several insect borne arboviruses but underrepresented in terms availability high-resolution genomic resources. We assembled and annotated complete mitochondrial genomes for two Culicoides species, namely C. brevitarsis imicola which proven Bluetongue Virus (BTV). used both long read short sequencing technologies to assemble the circular genomes. The genome sizes 17,100 bp 17,031 bp, respectively, all comprising 37 genes, including 13...
In recent years, novel Bluetongue virus (BTV) serotypes have been isolated and/or sequenced by researchers within the field. During surveillance activities, we identified a putative BTV serotype in healthy goats from Sardinia, Italy. RNAs purified blood and serum samples were positive for generic real time RT-PCR c-ELISA, respectively, whereas genotyping serotyping unsuccessful. By NGS, whole genome sequence was obtained two (BTV-X ITL2015 strains 34200 33531). Overall, Seg 2 of BTV-X shows...
There is strong evidence that severe acute respiratory syndrome 2 virus (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19) pandemic, originated from an animal reservoir. However, exact mechanisms emergence, host species involved, and risk to domestic agricultural animals are largely unknown. Some species, including cats, ferrets, minks, have been demonstrated be susceptible SARS-CoV-2 infection, while others, such as pigs chickens, not. Importantly, susceptibility...
Abstract It is unclear whether West Nile virus (WNV) circulates between Africa and Europe, despite numerous studies supporting an African origin high transmission in Europe. We integrated genomic data with geographic observations phylogenetic phylogeographic inferences to uncover the spatial temporal viral dynamics of WNV these two continents. focused our analysis towards lineages 1 (L1) 2 (L2), most spatially widespread pathogenic lineages. Our study shows a Northern-Western L1,...
Streptococcus equi subsp. zooepidemicus (SEZ) is a significant concern in equine veterinary medicine. Typically, commensal horses, SEZ can cause severe diseases including respiratory infections, septicemia, and reproductive tract infections under certain conditions. Recent evidence suggests that may also humans through direct contact with animals or consumption of unpasteurized milk dairy products. This study investigates strains isolated from nasal swabs Equidae central Italy 2023 to...
Purpose. From May 2015 to March 2016, an outbreak due Listeria monocytogenes serotype 1/2a and clinical pulsotype never previously isolated in Europe occurred central Italy, involving 24 confirmed cases. The article provides a description of the investigation carried out by multidisciplinary network. Methodology. Epidemiological microbiological surveillance was conducted confirm detect food vehicle infection. origin destination implicated its ingredients were investigated tracing-back...
A total of 66 Listeria monocytogenes (Lm) isolated from 2013 to 2018 in a small-scale meat processing plant and dairy facility Central Italy were studied. Whole Genome Sequencing bioinformatics analysis used assess the genetic relationships between strains investigate persistence virulence abilities. The biofilm forming-ability was assessed vitro. Cluster grouped Lm into three main clusters: two them, both belonging CC9, persisted for years one (CC121) last year sampling. In facility, all...
The maintenance of tick-borne disease agents in the environment strictly depends on relationship between tick vectors and their hosts, which act as reservoirs for these pathogens. A pilot study aimed to investigate wild rodents zoonotic pathogens (Borrelia burgdorferi sensu lato (s.l.), Coxiella burnetii, Francisella tularensis, Anaplasma phagocytophilum) was carried out an area Gran Sasso e Monti della Laga National Park (Abruzzi Region, central Italy), a wide protected where, despite...
During November 2021-May 2022, we identified 37 clinical cases of Streptococcus equi subspecies zooepidemicus infections in central Italy. Epidemiologic investigations and whole-genome sequencing showed unpasteurized fresh dairy products were the outbreak source. Early diagnosis by using technology prevented spread life-threatening S. subsp. infections.
Human granulocytic anaplasmosis (HGA) is an emerging tick-borne zoonosis worldwide. As the case for many diseases, epidemiological cycle associated to environmental conditions, including presence of wild vertebrate reservoir hosts, vectors, climate and vegetation. In this study a total number 87 spleen samples ruminants carcasses from Central Italy, 77 Ixodes ricinus collected same dead animals were screened Anaplasma phagocytophilum by using Real Time PCR. A. DNA was detected in 75%, 66.7%...
Abstract Prompt identification of the causative pathogen an infectious disease is essential for choice treatment or preventive measures. In this perspective, nucleic acids purified from brain tissue a dog succumbed after severe neurological signs were processed with MinION (Oxford Nanopore Technologies, Oxford UK) sequencing technology. Canine distemper virus (CDV) sequence reads detected. Subsequently, specific molecular test and immunohistochemistry used to confirm presence CDV RNA...
In Algeria, data on the epidemiology of coxiellosis in cattle are still lacking. this study, bulk tank milk (BTM) samples from 200 randomly selected dairy herds Setif province Algeria were analyzed by an indirect enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Results highlighted that 37% (95% CI: 30.31-43.69%) 9% 5.03-12.96%) BTM contained Coxiella burnetii antibodies DNA, respectively. Based Cohen's kappa coefficient, a very low agreement between ELISA PCR...
Microplastic contamination is a growing marine environmental issue with possible consequences for seafood safety. Filter feeders are the target species microplastic (MPs) pollution because they filter large quantities of seawater to feed. In present study, an experimental Mytilus galloprovincialis was conducted using mixture main types MPs usually in column (53% filaments, 30% fragments, 3% granules) order test purification process as potential method removing these contaminants from...
Coxiella burnetii is the causative agent of Q fever. The main reservoirs for this bacterium, which can lead to human infection, in our region are typically cattle, goats, and sheep. In animals, C. infection often detected due reproductive problems. European Member States required report confirmed cases annually, but lack uniform reporting methods makes data rather inconsistent. Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise involved official controls identify causes...
Listeria monocytogenes (Lm) can persist in food processing environments (FPEs), surviving environmental stresses and disinfectants. We described an intensive monitoring plan performed Central Italy involving producing plants (FPPs) retail grocery stores (RSs). The aim of the study was to provide a snapshot Lm circulation different FPEs during severe listeriosis outbreak, using whole genome sequencing (WGS) investigate genetic diversity isolated, evaluating their virulence stress resistance...
West Nile virus is a re-emerging arbovirus whose impact on public health increasingly important as more and epidemics epizootics occur, particularly in America Europe, with evidence of active circulation Africa. Because birds constitute the main reservoirs, migratory movements allow diffusion various lineages world. It therefore crucial to properly control dispersion these lineages, especially because some have greater than others. This work describes development validation novel...
The role of migratory birds in the spread ticks and tick-borne pathogens along their routes from Africa to Europe is increasingly emerging. Wild can host several tick species, often infected by bacteria responsible for zoonoses. aim study assess possible introduction exotic carried into Italy detect presence Rickettsia species Coxiella burnetii they may harbor. During a two-year survey, we collected captured during short stop-over on Ventotene Island. Specimens were first identified...