A5055085184- Mosquito-borne diseases and control
- Listeria monocytogenes in Food Safety
- Malaria Research and Control
- Vector-Borne Animal Diseases
- SARS-CoV-2 and COVID-19 Research
- Insect symbiosis and bacterial influences
- Microbial Inactivation Methods
- Bacteriophages and microbial interactions
- Essential Oils and Antimicrobial Activity
- Viral Infections and Vectors
- Hepatitis Viruses Studies and Epidemiology
- Animal Virus Infections Studies
- Identification and Quantification in Food
- Viral gastroenteritis research and epidemiology
- Dermatological diseases and infestations
- Vibrio bacteria research studies
- Molecular Biology Techniques and Applications
- Cytomegalovirus and herpesvirus research
- Microbial infections and disease research
- Hepatitis B Virus Studies
- SARS-CoV-2 detection and testing
- Vector-borne infectious diseases
- Fibroblast Growth Factor Research
- Liver physiology and pathology
- Mycobacterium research and diagnosis
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2018-2025
University of Teramo
2020
Abstract It is unclear whether West Nile virus (WNV) circulates between Africa and Europe, despite numerous studies supporting an African origin high transmission in Europe. We integrated genomic data with geographic observations phylogenetic phylogeographic inferences to uncover the spatial temporal viral dynamics of WNV these two continents. focused our analysis towards lineages 1 (L1) 2 (L2), most spatially widespread pathogenic lineages. Our study shows a Northern-Western L1,...
Abstract Background Primary Immunodeficiency disorders (PID) can increase the risk of severe COVID-19 and prolonged infection. This study investigates duration SARS-CoV-2 excretion genetic evolution virus in pediatric PID patients as compared to immunocompetent (IC) patients. Materials methods A total 40 nasopharyngeal 24 stool samples were obtained from five ten IC children. RNA detection was performed using RT-qPCR, whole-genome sequencing conducted with NexSeq 1000 platform. Data analysis...
Listeriosis, caused by Listeria monocytogenes , is a severe foodborne infection, particularly hazardous for pregnant women and immunocompromised individuals. In Europe, L. was the fifth most reported zoonotic agent in 2022, with outbreaks frequently linked to ready‐to‐eat (RTE) foods. Addressing food contamination innovative antimicrobial strategies critical enhancing safety reducing public health risks. This study evaluated efficacy of two bacteriophage‐derived endolysins, LP101_021...
In January 2022, West Nile virus (WNV) lineage 2 (L2) was detected in an adult female goshawk rescued near Perugia the region of Umbria (Italy). The animal showed neurological symptoms and died 15 days after its recovery a wildlife rescue center. This second case WNV infection recorded birds during cold season, when mosquitoes, main vectors, are usually not active. According to National Surveillance Plan, is included amongst low-risk areas. necropsy evidenced generalized pallor mucous...
Several lineages of SARS-CoV-2 are currently circulating worldwide. During diagnostic activities performed in Abruzzo region (central Italy) several strains belonging to the B.1.177.75 lineage tested negative for N gene but positive ORF1ab and S genes (+/+/- pattern) by TaqPath COVID-19 CE-IVD RT-PCR Kit manufactured Thermofisher. By sequencing, a unique mutation, synonymous 28948C > T, was found N-negative strains. Although we do not have any knowledge upon nucleotide sequences primers...
West Nile virus is a re-emerging arbovirus whose impact on public health increasingly important as more and epidemics epizootics occur, particularly in America Europe, with evidence of active circulation Africa. Because birds constitute the main reservoirs, migratory movements allow diffusion various lineages world. It therefore crucial to properly control dispersion these lineages, especially because some have greater than others. This work describes development validation novel...
Within public health control strategies for SARS-CoV-2, whole genome sequencing (WGS) is essential tracking viral spread and monitoring the emergence of variants which may impair effectiveness vaccines, diagnostic methods, therapeutics. In this manuscript different SARS-CoV-2 WGS including metagenomic shotgun (SG), library enrichment by myBaits® Expert Virus-SARS-CoV-2 (Arbor Biosciences), nCoV-2019 protocol, ampliseq approach Swift Amplicon® Panel kit (Swift Illumina COVIDSeq Test (Illumina...
Hepatitis E virus (HEV) is an emergent zoonotic pathogen, causing worldwide acute and chronic hepatitis in humans. HEV comprises eight genotypes several subtypes. 3 4 (HEV3 HEV4) are zoonotic. In Italy, the most part of infections (80%) due to autochthonous HEV3 circulation virus, key role played by wild animals generally accepted. Abruzzo Italian region officially considered "hot spot", with subtype HEV3-c being up now only one reported among boars. During year 2018-2019, a group boars were...
Abstract Epithelial-mesenchymal transition (EMT) is a complex biological program between physiology and pathology. Here, amniotic epithelial cells (AEC) were used as in vitro model of transiently inducible EMT order to evaluate the transcriptional insights underlying this process. Therefore, RNA-seq was identify differentially expressed genes enrichment analyses carried out assess intracellular pathways involved. As result, molecules exclusively AEC that experienced (GSTA1-1 GSTM3) or when...
Although there are increasing reports on the prevalence of Listeria monocytogenes in wild species, this is first case listeriosis sea turtle. An adult female Caretta caretta was rescued after being stranded alive along coast Abruzzo region (Italy) summer 2021. The turtle died 6 days due to respiratory failure. necropsy showed widespread organ lesions, such as yellow foci necrosis many organs, gastrointestinal erosions, pericarditis, and granulomatous pneumonia. Microbiological histological...
(BoAHV-1) infection is common in cattle worldwide. However, information on the spread of BoAHV-1-circulating strains Italy remains limited. In this study, we investigated an outbreak characterized by severe respiratory symptoms a herd (
Abstract Usutu virus (USUV), an arbovirus from the Flaviviridae family, genus Flavivirus , has recently gained increasing attention because of its potential for emergence. After his discovery in South Africa, USUV spread to other African countries, then emerged Europe where it was responsible epizootics. The been found Asia. infection humans is considered be most often asymptomatic or cause mild clinical signs. However, a few cases neurological complications such as encephalitis...
is a significant concern in the food industry due to its association with outbreaks of listeriosis, particularly affecting vulnerable populations. High-throughput technologies such as RNA sequencing (RNA-seq) and proteomics offer valuable insights into molecular responses
Abstract West Nile virus (WNV) is a mosquito-borne pathogen that can infect humans, equids, and many bird species, posing threat to their health. It consists of eight lineages, with Lineage 1 (L1) 2 (L2) being the most prevalent pathogenic. Italy one hardest-hit European nations, 330 neurological cases 37 fatalities in humans 2021–2022 season, which L1 re-emerged after several years low circulation. We assembled database comprising all publicly available WNV genomes, along 31 new Italian...
As the epidemiology of human Q Fever generally reflects spread Coxiella burnetii in ruminant livestock, molecular characterization strains is essential to prevent outbreaks. In this study we report genetic diversity C. central Italy accomplished by MST and MLVA-6 on biological samples from 20 goat, sheep cow farms.Five ten MLVA profiles emerged analysis establishing a part strain world atlas. particular, ST32 occurred 12 farms (60%), prevalently goat specimens, while ST12 (25%) was detected...
Epithelial-mesenchymal transition (EMT) changes cell phenotype by affecting immune properties of amniotic epithelial cells (AECs). The present study shows how the response to lipopolysaccharide collected pre- (eAECs) and post-EMT (mAECs) induces in their transcriptomics profile. In fact, eAECs mainly upregulate genes involved antigen-presenting response, whereas mAECs over-express soluble inflammatory mediator transcripts. Consistently, network analysis identifies CIITA Nrf2 as main drivers...
We report the chromosome and plasmid sequences of a strain Listeria monocytogenes IVb variant 1, recently emerging serotype, isolated in Italy from ready-to-eat vegetables.
Mycoplasma mycoides subsp. (Mmm) is the etiological agent of contagious bovine pleuropneumonia (CBPP), one major diseases affecting cattle in sub-Saharan Africa. Some evidences suggest that immune system host (cattle) plays an important role pathogenic mechanism CBPP, but factors involved process remain largely unknown. The present study aimed to investigate cell response polymorphonuclear neutrophils (PMNs) after Mmm vitro exposure using step RT-qPCR and Western blotting. Data obtained...
Bovine kobuvirus (BKV) is an infectious agent associated with neonatal calf diarrhoea (NCD), causing important economic losses to dairy and beef cattle herds worldwide. Here, we present the detection rate characterize genome of BKV isolated from diarrhoeic calves a Central Italy herd. From January December 2021, collected blood samples nasal rectal swabs 66 severe NCD between 3 20 days age. After virological (bovine coronavirus, bovine viral virus, rotavirus), bacteriological (Escherichia...
In this report, the draft genome sequence of Listeria monocytogenes serovar 1/2a strain IZSAM_Lm_14-16064, isolated in Italy from a cooked ham, is announced. The similar to that clinical 2014.
Abstract Background Hepatitis E virus (HEV) is a zoonotic pathogen and wild boars are recognised as reservoirs. HEV the most common cause of acute viral hepatitis worldwide it usually transmitted by faecal-oral route. EU reported more than 21.000 human cases over last 10 years (1). At present, comprises 8 genotypes several subtypes. 3 4 (HEV-3 HEV-4) zoonotic. This first report HEV-3 subtype e f in boar population Abruzzo region. Methods During hunting season 2018-2019, liver samples from...
Abstract Background Foodborne pathogens present in soil or irrigation water have the potential to internalize via root into edible parts of plants and survive, representing a serious hazard for consumer's health (1). The work assesses ability Salmonella Typhimurium (ST) enter basil (Ocimum basilicum L., cultivar “Napoletano”) from roots. This situation may represent risk food safety since that vegetable is largely used raw/undercooked, Mediterranean diet. Methods Sixteen were cultivated...