A5027277262- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Mycobacterium research and diagnosis
- Brucella: diagnosis, epidemiology, treatment
- Aquaculture disease management and microbiota
- Infectious Diseases and Mycology
- Animal Disease Management and Epidemiology
- Salmonella and Campylobacter epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Vectors
- Animal Diversity and Health Studies
- Trypanosoma species research and implications
- Burkholderia infections and melioidosis
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Escherichia coli research studies
- Plant Disease Management Techniques
- Myxozoan Parasites in Aquatic Species
- Listeria monocytogenes in Food Safety
- Prion Diseases and Protein Misfolding
- Microbial Inactivation Methods
- Galectins and Cancer Biology
- Plant and Fungal Interactions Research
- Plant Virus Research Studies
- Genetic and phenotypic traits in livestock
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2014-2024
Istituto Zooprofilattico Sperimentale delle Venezie
2024
International Livestock Research Institute
2015-2020
University of Bern
2018
Istituto Giordano
2011-2012
Royal Veterinary College
2010
Relatively little is known about regulatory T (Treg) cells and their functional responses in dogs. We have used the cross-reactive anti-mouse/rat Foxp3 antibody clone FJK-16s to identify a population of canine CD4+ FOXP3high both peripheral blood (PB) popliteal lymph node (LN). FOXP3+ PB LN yielded positive staining with newly developed anti-murine/human Helios 22F6, consistent notion that they were naturally occurring Treg cells. Stimulation mononuclear origin concanavalin A (Con A) vitro...
According to European Union (EU) regulations, the serological tests for eradication of bovine and ovine brucellosis are Rose Bengal Test, Complement Fixation i-ELISA. These methods, also recommended by World Organization Animal Health (OIE) international trades, have limitations related use suspensions smooth Brucellae or LPS extracts. Limitations include false-positive reactions brucellosis, which in turn impedes accurate diagnosis some herds. False positive should be considered carefully...
Abstract Background Contagious Bovine Pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. , is widespread in sub-Saharan Africa. The current live vaccine T1/44 has limited efficacy and occasionally leads to severe side effects the animals. A better understanding of immune responses triggered their role disease progression will help facilitate design a rational vaccine. Currently, knowledge cytokines involved immunity immunopathology CBPP rather limited. aim this study was...
Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides, is an important livestock disease in Africa. The current control measures rely on a vaccine with limited efficacy and occasional severe side effects. Knowledge of the protective arms immunity involved this will be beneficial for development improved vaccine. In previous studies cattle infected M. correlation was detected between levels mycoplasma-specific IFN-γ-secreting CD4+ T lymphocytes reduced...
From November 2016 to April 2017, a cross-sectional study determine the sero-prevalence of contagious caprine pleuropneumonia (CCPP) and investigate its epidemiology was conducted in selected districts Borana zone Ethiopia. In addition, aimed at identifying Mccp antigens using species specific primer PCR. A multistage random sampling implemented select districts, pastoral associations (villages), households. total 890 serum samples small ruminants that had not been vaccinated (goats n = 789...
Contagious caprine pleuropneumonia (CCPP), caused by Mycoplasma capricolum subsp. capripneumoniae is a severe disease widespread in Africa and Asia. Limited knowledge available on the pathogenesis of this organism, mainly due to lack robust vivo challenge model means do site-directed mutagenesis. This work describes establishment novel for CCPP that resulted 100% morbidity using combination repeated intranasal spray infection followed single transtracheal employing recent Kenyan outbreak...
Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. (Mmm) is a severe cattle disease, present in many countries sub-Saharan Africa. The development of improved diagnostic tests and vaccines for CBPP control remains research priority. Polyacrylamide gel electrophoresis mass spectrometry were used to characterize the Triton X-114 soluble proteome nine Mmm strains isolated from Europe or Of total 250 proteins detected, 67 all investigated. these, 44 predicted be...
Contagious bovine pleuropneumonia (CBPP), a severe respiratory disease of cattle caused by Mycoplasma mycoides subsp. (Mmm) is endemic in many African countries due to fragmented veterinary services and the lack an efficient vaccine sensitive diagnostics. More tools control are needed, but develop tools, better understanding host-pathogen interactions necessary. The aim this study was characterize kinetics humoral immune response against 65 Mmm surface antigens for extended period that...
Ovine and caprine brucellosis, caused by Brucella melitensis, is one of the world's most widespread zoonoses a major cause economic losses in domestic ruminant production. In Italy, disease remains endemic several southern provinces, despite an ongoing brucellosis eradication programme. this study, we used whole-genome sequencing to detail genetic diversity circulating strains, examine origins predominant sub-lineages B. melitensis Italy. We reconstructed global phylogeny strengthened 339...
Brucellosis is a contagious disease caused by bacteria of the genus Brucella, which can affect different animal species. Dogs may occasionally be infected with B. abortus, melitensis or suis, endemic form disease, canis. Among brucellosis‑affecting domestic animals, that dog certainly least frequent, but also studied. Canine brucellosis due to canis represents dog‑specific brucellosis, both because it main susceptible species, and constitutes its fundamental reservoir infection. The humans,...
Canine brucellosis caused by Brucella canis, is an infectious disease affecting dogs and wild Canidae. Clinical diagnosis challenging, laboratory testing crucial for a definitive diagnosis. Various serological methods have been described, but their accuracy uncertain due to limited validation studies. The present study aimed evaluate the performances of three tests B. canis in comparison with bacterial isolation (gold standard), order establish protocol canine brucellosis. A panel sera from...
Capsular polysaccharides have been confirmed to be an important virulence trait in many gram-positive and gram-negative bacteria. Similarly, they are proposed traits minimal Mycoplasma that cause disease humans animals. In the current study, goats were infected with caprine pathogen mycoides subsp. capri or engineered mutant lacking capsular polysaccharide, galactofuranose. Goats strain showed only transient fever. contrast, 5 of 8 parental reached end-point criteria after infection. These...
Contagious bovine pleuropneumonia (CBPP) is a severe disease caused by Mycoplasma mycoides subsp. (Mmm). Knowledge on CBPP pathogenesis fragmented and hampered the limited availability of laboratory animal in vitro models investigation. The purpose present study to assess respiratory explants as useful tools early stages CBPP. Explants were obtained from trachea, bronchi lungs slaughtered cattle, tested negative for spp. major bacterial viral pathogens. interaction Mmm with explant cells was...
Contagious bovine pleuropneumonia (CBPP), a severe respiratory disease, is characterized by massive inflammation of the lung especially during acute clinical stage infection. Tissue samples from cattle, experimentally infected with Mycoplasma mycoides subsp. Afadé, were subjected to histopathological and immunohistochemical examination in order provide insight into innate immune pathways that shape inflammatory host responses. Lung lesions vasculitis, necrosis, increased presence macrophages...
The significance of Trypanosoma equiperdum as the causative agent dourine cannot be understated, especially given its high mortality rate among equids. International movement equids should subject to thorough health checks and screenings ensure that animals are not infected with equiperdum. This involves implementation quarantine protocols, testing procedures, issuance certificates certify status animals. Three proteins, peptidyl-prolyl cis-trans isomerase (A0A1G4I8N3), GrpE protein homolog...
is a significant concern in the food industry due to its association with outbreaks of listeriosis, particularly affecting vulnerable populations. High-throughput technologies such as RNA sequencing (RNA-seq) and proteomics offer valuable insights into molecular responses
Animal diseases due to mycoplasmas are a major cause of morbidity and mortality associated with economic losses for farmers all over the world. Currently used mycoplasma vaccines exhibit several drawbacks, including low efficacy, short time protection, adverse reactions, difficulty in differentiating infected from vaccinated animals. Therefore, there is need improved control animal mycoplasmoses. Here, we genome engineering tools derived synthetic biology approaches produce targeted...
Human salmonellosis incidence is increasing in the European Union (EU). Salmonellaenterica subsp. enterica serovar Enteriditis, Typhimurium (including its monophasic variant) and Infantis represent targets control programs due to their frequent association with human cases. This study aimed detect most prevalent serotypes circulating Abruzzo Molise Regions between 2015 2020 framework of Italian National Control Program for Salmonellosis Poultry (PNCS)]. A total 332 flocks were sampled by...