A5044634771- Brucella: diagnosis, epidemiology, treatment
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Trypanosoma species research and implications
- Monoclonal and Polyclonal Antibodies Research
- Microbial infections and disease research
- Viral Infections and Vectors
- Biosensors and Analytical Detection
- Identification and Quantification in Food
- vaccines and immunoinformatics approaches
- Toxin Mechanisms and Immunotoxins
- Escherichia coli research studies
- Parasitic Infections and Diagnostics
- Burkholderia infections and melioidosis
- Listeria monocytogenes in Food Safety
- Infectious Diseases and Mycology
- Galectins and Cancer Biology
- Vector-borne infectious diseases
- Viral gastroenteritis research and epidemiology
- Mycobacterium research and diagnosis
- Yersinia bacterium, plague, ectoparasites research
- Viral Infectious Diseases and Gene Expression in Insects
- Helminth infection and control
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2015-2025
Rose Bengal antigen and smooth lipopolysaccharide (s-LPS) were produced from a field strain of Brucella ceti (“homologous” antigens) the reference B. abortus S99 (“heterologous” antigens); they are currently used for diagnosis brucellosis in cattle, water buffaloes, sheep, goats, pigs, as recommended Manual Diagnostic Tests Vaccines Terrestrial Animals World Organization Animal Health (WOAH). “Homologous” “heterologous” antigens rapid serum agglutination test (Rose test, RBT) competitive...
Summary A competitive ELISA ( Brucella ‐Ab c‐ELISA) was standardized and validated for the detection of antibodies in cattle, sheep goat sera using a monoclonal antibody (MAb 4B5A) produced against melitensis biotype 2. The specificity sensitivity assay were 100% to 67.5% cut‐off point (B/Bo%). When compared with an indirect ELISA, c‐ELISA did not demonstrate cross‐reactions when testing positive some Enterobacteriaceae. comparison made between complement fixation Rose Bengal tests. Results...
Canine brucellosis caused by Brucella canis, is an infectious disease affecting dogs and wild Canidae. Clinical diagnosis challenging, laboratory testing crucial for a definitive diagnosis. Various serological methods have been described, but their accuracy uncertain due to limited validation studies. The present study aimed evaluate the performances of three tests B. canis in comparison with bacterial isolation (gold standard), order establish protocol canine brucellosis. A panel sera from...
Epizootic hemorrhagic disease virus serotype 8 (EHDV-8) emerged in Europe for the first time late 2022. In this study, we investigated kinetics of EHDV-8 infection cattle, sheep, and goats. Following experimental with EHDV-8, four out five calves displayed fever, while another calf exhibited ulcerative crusty lesions muzzle. RNAemia peaked at day 7 post all remained relatively stable till end 78 days infection. Infectious was isolated up to 21 one calf. As far as small ruminants are...
Monoclonal antibodies (MAbs) specific for the lipopolysaccharide (LPS) of Escherichia coli O104:H4 were produced by fusion Sp2/O-Ag-14 mouse myeloma cells with spleen Balb/c mice, immunized heat-inactivated and sonicated E. bacterial cells. Four MAbs LPS (1E6G6, 1F4C9, 3G6G7 4G10D2) characterized evaluated use in a method detection milk samples that involves antibody conjugation to magnetic microbeads reduce time increase efficiency isolation. MAb 1E6G6 was selected coupled microbeads, then...
The diagnosis of dourine can be difficult because the clinical signs this disease in horses are similar to those surra, caused by Trypanosoma evansi. Moreover, T. equiperdum and evansi closely related and, so far, they cannot distinguished using serological tests. In a previous work, protein pattern recognized antibodies from dourine-infected humoral immune response kinetics were investigated immunoblotting assay; total 20 sera naturally experimentally infected healthy animals tested....
The significance of Trypanosoma equiperdum as the causative agent dourine cannot be understated, especially given its high mortality rate among equids. International movement equids should subject to thorough health checks and screenings ensure that animals are not infected with equiperdum. This involves implementation quarantine protocols, testing procedures, issuance certificates certify status animals. Three proteins, peptidyl-prolyl cis-trans isomerase (A0A1G4I8N3), GrpE protein homolog...
Four hybridoma cell lines (C4B, 10C2G5, 6C5F4C7, 2D10G11) were adapted to grow in serum-free conditions. Cell proliferation, viability, and antibody production Nutridoma SP Ex-Cell HSF 610 media evaluated results compared with those obtained using DMEM containing 10% fetal bovine serum (control medium). Clone C4B showed the best IgG productivity control medium, but viability number of cells per milliliter similar for three media. For clone highest values both medium SP; no significant...
Listeria monocytogenes is one of the main foodborne pathogens worldwide. Although its response to stress conditions has been extensively studied, it still present in food processing environments and a concern for consumers. To investigate how this microorganism adapts proteome mild conditions, combined proteomics bioinformatics approach was used characterize immunogenic protein profile sequence type 7 (ST7) strain that caused severe listeriosis outbreaks central Italy. Extracted proteins...
An immunohistochemical (IHC) technique was optimised using a monoclonal antibody (MAb) to detect Mycoplasma mycoides subsp. (Mmm), the agent of Contagious Bovine Pleuropneumonia (CBPP), in sections lung tissue. A panel MAbs produced and screened for Mmm speci city cross-reactivity against other mycoplasmas belonging not cluster, parallel indirect ELISA (i-ELISA) Immunoblotting (IB). Based on i-ELISA IB characterization data, 1 MAb (clone 3G10E7) selected its highest nity vs con rmed by...
Brucella RB51 is a live modified vaccine. Its use in water buffalo has been proposed using vaccination protocol different to that used for cattle, but knowledge of the long-term effects this species remains incomplete. The aim study was evaluate safety and kinetics antibody responses buffaloes vaccinated according described bovine WOAH Manual, with triple dose. Water were vaccine RB51. A booster administered at 12 months age. When turning 23-25 old, female animals induced pregnancy....
Bacteria of the genus Brucella cause brucellosis, an infectious disease common to humans as well terrestrial and aquatic mammals. Since 1994 several cases spp. infection have been reported in marine mammals worldwide. While sero-epidemiological data suggest that is widespread globally, detecting spp.-associated antigens by immunohistochemistry (IHC) tissues from infected animals often troublesome. The present study was aimed at investigating, means IHC based upon utilization anti-Brucella...
Monoclonal antibodies (MAbs) against horse IgG were produced by immunizing Balb/c mice with purified and characterized in indirect ELISA versus immunoglobulins from donkey, cow, buffalo, sheep, pig, chicken. Three MAbs (1B10B6C9, 1B10B6C10, 1B10B6E9) reacted only donkey IgM and, western blotting, specific for the Fc fragment of equine IgG. MAb 1B10B6E9 was used chemiluminescent immunoblotting assay diagnosis dourine immunofluorescence (IFA) African sickness dourine.
Brucella canis is pathogenic for dogs and humans. Serological diagnosis a cost-effective approach disease surveillance, but major drawback of current serological tests the cross-reactivity with other bacteria that results in false positive reactions. Development indirect improved sensitivity specificity use selected B. proteins instead whole antigen remain priority.A western blotting assay was developed to define serum antibody patterns associated infection using panel negative dog sera....
Brucella canis is pathogenic for dogs and humans. Serological diagnosis a cost-effective approach disease surveillance, but major drawback of current serological tests the cross-reactivity with other bacteria that results in false positive reactions, development indirect improved sensitivity specificity remain priority. A western blotting assay was developed to define serum antibody patterns associated infection using panel negative dog sera. B. sera recognized immunogenic bands ranging from...