A5082178462- Clostridium difficile and Clostridium perfringens research
- Microbial infections and disease research
- Viral gastroenteritis research and epidemiology
- Streptococcal Infections and Treatments
- Animal Disease Management and Epidemiology
- Respiratory viral infections research
- Bacteriophages and microbial interactions
- Veterinary Oncology Research
- Toxin Mechanisms and Immunotoxins
- Microscopic Colitis
- Viral Infections and Vectors
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Neonatal Respiratory Health Research
- Congenital Diaphragmatic Hernia Studies
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Mycobacterium research and diagnosis
- Wnt/β-catenin signaling in development and cancer
- Gut microbiota and health
- Vector-borne infectious diseases
- Animal Behavior and Welfare Studies
- Botulinum Toxin and Related Neurological Disorders
- Aquaculture disease management and microbiota
- Veterinary medicine and infectious diseases
University of Bern
2016-2025
Abstract Japanese encephalitis virus (JEV), a main cause of severe viral in humans, has complex ecology, composed cycle involving primarily waterbirds and mosquitoes, as well pigs amplifying hosts. To date, JEV transmission been exclusively described being mosquito-mediated. Here we demonstrate that can be transmitted between the absence arthropod vectors. Pigs shed oronasal secretions are highly susceptible to infection. Clinical symptoms, tropism central nervous system histological lesions...
Pigs are considered to be the main amplifying host for Japanese encephalitis virus (JEV), and their infection can correlate with human cases of disease. Despite importance in ecology as it relates encephalitis, pathogenesis JEV pigs remains obscure. In present study, localization kinetics replication were investigated various tissues after experimental intravenous pigs. The data demonstrate a rapid broad spreading central nervous system (CNS) other organs. A particular tropism not only CNS...
African Swine Fever virus (ASFV) is a large double-enveloped DNA of the Asfarviridae family that causes lethal hemorrhagic disease in domestic pigs and wild boars. Since 2007, highly virulent genotype II strain has emerged spread Europe South-East Asia, where millions animals succumbed to disease. Field- laboratory-attenuated strains ASFV cause variable clinical severity survival, mechanisms remain unclear. We hypothesized immunological hygienic status determinant ASF course. Here we...
Clostridium perfringens β-toxin (CPB) is a β-barrel pore-forming toxin and an essential virulence factor of C. type C strains, which cause fatal hemorrhagic enteritis in animals humans. We have previously shown that CPB bound to endothelial cells within the intestine affected pigs humans, highly toxic primary porcine (pEC) vitro. The objective present study was investigate cell death induced by these cells, potential host mechanisms involved this process. rapidly lactate dehydrogenase (LDH)...
Coxiella burnetii, Chlamydia abortus and Leptospira spp. are difficult to grow bacteria that play a role in bovine abortion, but their diagnosis is hampered by obligate intracellular lifestyle (C. C. abortus) or lability (Leptospira spp.). Their importance based on the contagious spread food-producing animals, also as zoonotic agents. In Switzerland, first-line routine bacteriological diagnostics cattle abortions regulated national law includes only basic screening staining for burnetii due...
Hepatozoon silvestris is an emerging apicomplexan parasite discovered in European wild cats from Bosnia and Herzegovina blood samples of a domestic cat Southern Italy 2017. It has also been identified Ixodes ricinus collected Wales, UK, 2018. The clinical relevance, pathogenesis epidemiology this novel species are not yet understood. Thus, the objective paper was to report describe first fatal case H. infection cat.The cat, which originated Switzerland, died shortly after presenting signs...
Abstract The Mycoplasma Immunoglobulin Binding/Protease (MIB-MIP) system is a candidate ‘virulence factor present in multiple pathogenic species of the Mollicutes , including fast-growing feriruminatoris . MIB-MIP cleaves heavy chain host immunoglobulins, hence affecting antigen-antibody interactions and potentially facilitating immune evasion. In this work, using -omics technologies 5’RACE, we show that four copies M. have different expression levels are transcribed as operons controlled by...
Seventy-two lynx, found dead in the Swiss Alps and Jura Mountains (Switzerland) from 1987-99, were evaluated to determine cause of death. per cent (52/72) all animals died because noninfectious diseases or causes such as vehicular collision poaching. Eighteen percent (13/72) infectious diseases, including some which could have been transferred lynx domestic other wild panleukopenia sarcoptic mange. If only radio-tagged (included a monitoring program) taken into consideration, percentage...
PEDV is a swine pathogen that responsible for significant animal and economic losses worldwide. Highly pathogenic variants can lead to mortality rate of up 100% in newborn piglets.
Sequence analysis of the adhesion molecule E-cadherin had revealed a multibasic motif [4PArg-Gln-Lys-Arg1P], reminiscent minimal cleavage signal for furin, prototype proprotein convertase family, and/or other members sharing similar sequence specificity. Mutation this site was sufficient to abolish processing in fibroblasts reinforcing possibility that convertases are involved maturation molecule. Here we demonstrate even though furin can efficiently and specifically cleave proE-cadherin...
Clostridium perfringens type C isolates cause fatal, segmental necro-hemorrhagic enteritis in animals and humans. Typically, acute intestinal lesions result from extensive mucosal necrosis hemorrhage the proximal jejunum. These are frequently accompanied by microvascular thrombosis affected segments. In previous studies we demonstrated that there is endothelial localization of C. beta-toxin (CPB) necrotizing enteritis. This led us to hypothesize CPB contributes vascular directly damaging...
A 10-year-old male, neutered domestic shorthair cat was presented with fever, anorexia, vomiting, and diarrhea. Serologic testing for Feline immunodeficiency virus leukemia were negative. Fine-needle aspirates of mesenteric lymph nodes revealed the presence banana-shaped apicomplexan parasites. The died after 4 days hospitalization. Postmortem polymerase chain reaction (PCR) analysis confirmed Toxoplasma gondii in all examined organs. Parasites ex vivo isolated outbred mice subsequently...
Animal health data recorded in free text, such as necropsy reports, can have valuable information for national surveillance systems. However, these are rarely utilized because the text format requires labor-intensive classification of records before they be analyzed with using statistical or other software. In a previous study, we designed text-mining tool to extract from reports. current used reports pig and cattle necropsies performed between 2000 2011 at Institute Pathology (ITPA),...
Mycoplasmas are the smallest free-living organisms and cause a number of economically important diseases affecting humans, animals, insects plants. Here, we demonstrate that highly virulent Mycoplasma mycoides subspecies capri (Mmc) can be fully attenuated via targeted deletion non-essential genes encoding, among others, potential virulence traits. Five genomic regions, representing approximately ten percent original Mmc genome, were successively deleted using Saccharomyces cerevisiae as an...
Abstract African Swine Fever virus (ASFV) is a large double-enveloped DNA of the Asfarviridae family that causes lethal hemorrhagic disease in domestic pigs and wild boars. Since 2007, highly virulent genotype II strain has emerged spread Europe South-East Asia, where millions animals succumbed to disease. Field- laboratory-attenuated strains ASFV cause variable clinical severity survival, mechanisms remain unclear. We hypothesized immunological hygienic status determinant ASF course. Here...
Despite the pivotal role of β-catenin in a variety biological processes, conditional gene ablation skin transgenic mice failed to affect interfollicular epidermal morphogenesis. We elucidated molecular mechanisms underlying this phenomenon. Long-term cultures homozygous, heterozygous and β-catenin-null mutant keratinocytes were established demonstrate that keratinocyte proliferation, cell cycle progression cyclin D1 expression occur independently correlate with repression transcription from...